LOCUS       sequence-181041-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear Huntingtin Chromobody 2: ""iVHH4"" intrabody recognizing 
            amino acids 49–148 of huntingtin, fused to eYFP and nuclear 
            localization signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Maiuri T, Mocle AJ, Hung CL, Xia J, van Roon-Mom WM, Truant R
  TITLE     Huntingtin is a scaffolding protein in the ATM oxidative DNA damage 
            response complex.
  JOURNAL   Hum Mol Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395.
  PUBMED    28017939
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395."
COMMENT     Sequence Label: nucHCB2
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(26..736,737..739,740..742))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMXLL
                     EFVTAAGITLGMDELYK"
     primer_bind     417..436
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     676..697
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cctttctctt cttcttgggt ctagacttgt acagctcgtc catgccgaga gtgatcccgg
       61 cggcggtcac gaactccagc aggnccatgt gatcgcgctt ctcgttgggg tctttgctca
      121 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      181 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      241 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      301 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      361 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      421 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      481 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc
      541 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      601 tgaacttcag ggtcagcttg ccgtangtgg catcgccctc gccctcgccg gacacgctga
      661 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      721 gctcctcgcc cttgctcacc atggatcctc ctgaggagac ggtgacctgg gtcccctggc
      781 cccagtagtc ataataccca ttacaatagt cgctataggg agcgcggact gtcgcacagt
      841 aataaacggc cgtgtcctca ggtttcaggc tgttcatttg cagatacacc gtgttcttgg
      901 cattgtctct ggagatggtg aatcggccct tcacggagtc tgcatagtat gtgctacnat
      961 cactactact aatacatgag accccctcgc gctccttccc tggggcctgg nngaacnngc
     1021 ctatggcata ntaatccaaa gtgaatccng aggctgcaca gga
//
