LOCUS       sequence-181052-       13400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 13400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3486 (YCp LEU2 Rpb1 F1492A)
FEATURES             Location/Qualifiers
     source          1..13400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..37
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     29..46
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     30..46
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7152..7170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7191..7207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7191..7207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7204..7226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7215..7231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7239..7245,7246..7263,7264..7269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7284..7305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7422..7439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7593..8181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7673..7692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8352..9143,9144..9212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9213..9317)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(9654..9758)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9795..10298
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(10396..10418)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10518..10537
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(11031..12125)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(12126..12533)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(12834..13289)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12971..12992)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13183..13202
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
       61 ctcactatag ggcgaattgg agctcttggg aagcttcagt accgcgattt ttcagctaat
      121 ggatcagatg atatataaac aaaaagtggg atacagcata tcgacgcctc cactacaaat
      181 taacagaagg atgaaaacca ataagaaaat ttctaaaaga agaagcctga aaaatctcca
      241 tggtgcctta aaaggactat tgaaagagtc cggcaagaag agtgagtcca aaatacgaaa
      301 acatagcgat tgcaatcctg ttcatcgagt atatccgcca aatatcgaaa aacggaagac
      361 caaaaaggac gatggaattt cacggccaat tgctgaaaga aatggtcatg tgtatataat
      421 gtccaaggaa aaccacatca tccctaaact aaccgatgat gaagtcatgg agcgccacaa
      481 actagcagac gaaaacatga gaaaagtgtg gtccaacatc ataagcaaat acgagtctat
      541 tgaagaacaa ggagacctcg tagacctgaa aactggtgaa atagttgaag ataatgggca
      601 tatcaagacg ctgacggcca acaacagcac gaaagataaa agaacaaaat acacaagcgt
      661 gctcagagac attatcgaca tcagcgacga ggaagatggt gataaaaatg atgaatatac
      721 tctttgggct aacgatagcg aggcaagcga ttcagaggtg gatgctgaca acgacactga
      781 agaggaaaaa gacgagaaat tgatagacgc agatttcaag aagtacgagg ccaaactctc
      841 gaaaaggata ttacgagatt aaggtgctaa aatgccgtat attcgaattt cattaccatc
      901 agaatatata tatatatata tgagtctata ttagggacta tcatacggta acccggacct
      961 ccattacccg catgacttta ctcactaaag acgattgtcg gttttaaaca tttttttttt
     1021 ttcatttcga ccgtcggttc tccttagttt gttcagtggc aacaagtgga caaacaagaa
     1081 gagcgtgcag caccattcac cgccagcttt gtatcgtcct ttagagtgca gcaagaacat
     1141 taattgctct cacagaggta tcataagaac atccgattcg ttcattaact gaaatcatat
     1201 ctgtgttgtt ctgatcgact ctctttgtta gttttatctt ccattttttt ttattccttt
     1261 cattttccaa taaaatttga acaggatcga tataaacaga taaaaaccct ccaaagttaa
     1321 ccatttgttt atatttgctt ttctgcataa gttttcctag cgaattccct gatcaacttt
     1381 caaggaaaac taaaactact gtattataag aggttttttt tttcattcca gattaatttt
     1441 gaaatacgat atcctcaagt ttatctacca gaatatttga ctaagaaatc aaactctgtt
     1501 aataataata taattataaa aacctcaact agaaactcca aaaaaaaaaa tttaccattt
     1561 tttactttct atccttgtta accaaatttc aaaaaaattt taccttttct ttttccagaa
     1621 gagggaccaa tcataaagat agcaataaca ctttacccca aaatataaat cagacatggt
     1681 aggacaacag tattctagtg ctccactccg tacagtaaaa gaggtccaat tcggtctttt
     1741 ctcacctgaa gaagttagag caatcagtgt ggccaaaatt agatttccag agacaatgga
     1801 tgaaacccag acgagagcga aaattggtgg tctaaacgac cctaggttag gctctattga
     1861 tcgtaatctg aagtgtcaaa cttgtcaaga gggtatgaac gaatgtcctg gtcattttgg
     1921 tcacatagat ttagcaaaac ctgtatttca tgttggtttt attgccaaaa ttaagaaagt
     1981 atgtgagtgt gtctgtatgc actgtggtaa gctattactg gatgaacata atgaattaat
     2041 gagacaagct ctagcaatca aagacagtaa aaaaaggttt gctgcaattt ggactttatg
     2101 taaaacaaaa atggtctgcg aaacagatgt cccttctgaa gatgatccta ctcagctcgt
     2161 atcaagggga ggttgtggta atacacagcc tacaattcgt aaggatgggt tgaaattagt
     2221 tggtagttgg aaaaaagata gagccacggg ggatgcggat gaaccagaac taagagtttt
     2281 aagtacggag gaaatcttga atatttttaa gcatatctca gtaaaagact tcactagttt
     2341 gggtttcaac gaagtttttt ctcgtccaga atggatgatt ttaacatgcc ttcctgtccc
     2401 accaccaccg gtgcgtccat ccatttcctt caatgaatct caaagaggtg aggatgattt
     2461 aacctttaaa cttgctgata ttttaaaagc taatattagt ttggaaacac tagagcataa
     2521 cggtgctcca catcatgcta ttgaagaagc agagagttta ttacaatttc atgttgccac
     2581 ttatatggat aatgatattg ctggtcaacc acaagctctg cagaagtccg gccgtcccgt
     2641 taaatctatt cgtgctcgtt tgaagggtaa agagggtcgt atcagaggta atttaatggg
     2701 taagcgtgtg gatttttcgg caagaactgt tatttctggt gatcctaatt tggaattaga
     2761 ccaagtcggt gttccaaaat ctattgccaa gactttaaca tacccagaag tggtcacacc
     2821 atataacata gatcgtctga cgcaacttgt taggaatgga ccaaatgagc accccggtgc
     2881 caaatacgtc attcgtgata gcggagaccg tatagattta agatacagta aaagggcagg
     2941 tgatattcaa ttacagtatg ggtggaaagt tgaacgtcat attatggaca atgatccagt
     3001 tttattcaac cgtcaacctt cgttgcacaa aatgtccatg atggcccaca gagtaaaagt
     3061 tattccatat tctacattta gattgaattt gtccgttaca tctccataca atgccgattt
     3121 cgacggtgac gaaatgaatc ttcacgttcc tcagtctgag gaaacaaggg cggaactttc
     3181 tcaattatgt gctgttcctc tgcaaattgt ttcaccacaa tctaacaaac cttgtatggg
     3241 tattgttcaa gatactttgt gtggtattcg taaactgaca ttaagagata catttataga
     3301 acttgatcaa gttttgaata tgctttattg ggttccagat tgggatggtg ttattccgac
     3361 acctgcaatt atcaagccca aacctttgtg gtccggtaaa caaatcttgt ctgtggctat
     3421 cccaaacggt attcatttac aacgttttga tgagggcact actctgcttt ctccaaagga
     3481 taatggtatg cttattattg acggtcaaat catttttggt gtagtagaga aaaaaaccgt
     3541 tggatcctcc aatggtggtt taattcatgt tgttacgaga gaaaagggac ctcaagtttg
     3601 tgctaagttg tttggtaaca tacagaaagt tgttaacttt tggttactac ataatgggtt
     3661 ttcaacaggt attggtgata ccattgcgga cggcccaaca atgagggaaa ttacagagac
     3721 aattgcagag gctaaaaaga aagttttgga tgttacgaaa gaagcccagg caaacttatt
     3781 gactgctaaa catggtatga ctctccgtga gtcttttgag gataacgttg ttcggttcct
     3841 aaatgaagca agagataagg caggtcgttt agctgaagtc aatttgaaag atttgaacaa
     3901 tgtgaaacaa atggttatgg caggttccaa gggttcattt attaatatcg cgcaaatgtc
     3961 agcttgtgta ggacagcaat ctgttgaagg taaacgtatt gcttttgggt tcgttgatcg
     4021 taccttacct catttctcta aagatgatta ctccccagag tctaaaggtt ttgttgagaa
     4081 ctcatatttg agaggtttga ccccacaaga attttttttc catgcaatgg gtggtcgtga
     4141 aggtcttatc gataccgccg tcaaaacagc cgaaacaggt tatattcaac gtcgtttagt
     4201 gaaagctcta gaagatatca tggttcatta cgataacacc acaagaaact cattgggtaa
     4261 cgttattcag tttatttatg gtgaagatgg tatggatgct gcgcatattg aaaagcaatc
     4321 gctagatact attggtggct ccgatgcagc ttttgaaaag agatacagag ttgatttatt
     4381 gaatacagac catacccttg atccctcact attggaatcc ggatctgaga tacttggcga
     4441 tttgaaactt caagttctcc tggatgaaga atacaaacaa ttagtgaaag atcgtaaatt
     4501 tttgagggaa gtttttgttg atggtgaagc aaactggcca ttaccagtca acataagacg
     4561 tattattcaa aatgctcaac aaactttcca catagatcat acgaaaccat ctgatttaac
     4621 aatcaaagac atcgttcttg gtgtaaagga tttgcaagaa aacttattag tgttgcgtgg
     4681 taagaatgaa attatacaaa atgcccagcg agatgcagtt acattgttct gctgtttatt
     4741 acgttcccgt ttggccacac gtagagttct acaagagtac agactaacaa aacaggcatt
     4801 cgattgggta ttaagtaata tcgaggcaca attcctccgt tctgttgttc accctggtga
     4861 aatggttggt gttctagcag cccaatccat tggtgaacca gccacacaaa tgacccttaa
     4921 caccttccat tttgctggtg ttgcttccaa aaaagttact tctggtgtcc cccgtttaaa
     4981 ggaaattttg aatgtggcca aaaacatgaa aaccccttcc ttgactgtat acttagagcc
     5041 cgggcatgct gccgatcaag aacaagcgaa gttgatcaga tctgctatcg agcataccac
     5101 tttaaagagt gtcactattg cttcagaaat ttactatgat cctgatccac gttccacagt
     5161 tattccagaa gatgaagaaa ttatccaact tcatttctca ttattggatg aagaggctga
     5221 acaatctttt gaccaacaat caccttggtt attacgtctg gaactggatc gtgcagcaat
     5281 gaatgataaa gacttaacaa tgggtcaggt tggtgaaaga atcaagcaaa cattcaaaaa
     5341 tgatttgttt gttatctggt ctgaagacaa cgatgagaag ttgatcatcc gttgtcgtgt
     5401 tgttcgtcca aagtcactag atgctgagac tgaagcagaa gaagatcata tgttgaagaa
     5461 aattgagaac acaatgttag agaatattac attacgtggt gtagagaaca tcgagcgtgt
     5521 tgtcatgatg aaatatgacc gtaaagtacc aagtccaact ggtgaatacg ttaaggaacc
     5581 tgaatgggtg ttggaaacag atggtgttaa cttatctgaa gttatgactg ttcctggtat
     5641 cgacccaacc agaatctata ccaactcctt cattgatata atggaagttc taggtattga
     5701 agctggtcgt gcagccttgt ataaagaagt ttacaatgtt attgcttctg atggttcgta
     5761 tgttaactac cgtcatatgg ctttgttagt cgatgttatg acaacccaag gtggcttaac
     5821 ttctgttact cgtcatggtt tcaacagatc aaatacaggt gccttaatga gatgttcatt
     5881 tgaagaaact gtcgaaattt tgtttgaagc tggtgcttca gccgaattag atgattgtcg
     5941 tggtgtttcg gaaaatgtca ttcttggtca aatggctcca atcggtaccg gtgcatttga
     6001 tgtgatgatc gatgaggagt cactggtaaa atacatgcca gaacaaaaaa taactgagat
     6061 tgaagacgga caagatggtg gcgtcacacc atacagtaac gaaagtggtt tggtcaatgc
     6121 agatcttgac gttaaagatg agctaatggc atcacctctg gttgattcgg gttcaaatga
     6181 cgctatggct ggaggattta cagcgtacgg tggtgctgat tatggtgaag ccacgtctcc
     6241 atttggtgct tatggtgaag cacctacatc tcccggattt ggagtctcct caccaggctt
     6301 ttctccaact tccccaacat actctcctac ctctccagcg tactcaccaa catcaccatc
     6361 gtactcacca acatcaccat cgtactcgcc aacatcacca tcgtactcac ctacatcacc
     6421 atcgtattca ccaacgtcac catcatattc gccaacgtca ccatcatatt cgccaacgtc
     6481 gccatcgtat tctccaacgt caccatcgta ttcgccaacg tcgccttcct actctcccac
     6541 gtcgccaagc tacagcccta cgtctccttc ttattctcct acatctccat catactctcc
     6601 tacgtcacca agttacagcc caacgtcacc aagttacagc ccaacgtctc cagcctattc
     6661 cccaacatca ccaagttata gtcctacatc gccttcatac tctccaacat caccatccta
     6721 ttccccaaca tcaccttctt actctcccac ctctccaaac tatagcccta cttcaccttc
     6781 ttactcccca acatctccag gctacagccc aggatctcct gcatattctc caaagcaaga
     6841 cgaacaaaag cataatgaaa atgaaaattc cagatgatat agtatatcat ccttacgtat
     6901 ttgacgttat tacattatat atagtttctc aaataatatt tctagtttat ttttgtatca
     6961 taataaaaac gtataccaaa tataccatta tttttcatag cattatggta gggataggga
     7021 atcaagtaac taatttatat ccgcagagca ttgggaaaac caacggcgct agtaaatgca
     7081 tttaaattac gtccgtccaa cttctaagct taagggtttc tcgagggggg gcccggtacc
     7141 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
     7201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9361 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctggataat
     9421 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9481 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9541 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9601 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9661 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9721 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9781 cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa
     9841 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
     9901 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc
     9961 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
    10021 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
    10081 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
    10141 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
    10201 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
    10261 aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat
    10321 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10381 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10441 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10501 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatcgacta
    10561 cgtcgttaag gccgtttctg acagagtaaa attcttgagg gaactttcac cattatggga
    10621 aatggttcaa gaaggtattg acttaaactc catcaaatgg tcaggtcatt gagtgttttt
    10681 tatttgttgt attttttttt ttttagagaa aatcctccaa tatataaatt aggaatcata
    10741 gtttcatgat tttctgttac acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt
    10801 aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac
    10861 ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata
    10921 tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
    10981 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
    11041 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
    11101 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
    11161 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
    11221 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
    11281 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
    11341 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
    11401 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
    11461 tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac agtttttctc
    11521 cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc
    11581 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
    11641 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
    11701 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
    11761 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
    11821 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact accggtaccc
    11881 catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga ggcttccagc
    11941 gcctcatctg gaagtggaac acctgtagca tcgatagcag caccaccaat taaatgattt
    12001 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
    12061 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca
    12121 gacattagaa tggtatatcc ttgaaatata tatatatata ttgctgaaat gtaaaaggta
    12181 agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta
    12241 aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat
    12301 tctatatgaa aagccggttc cggcgctctc acctttcctt tttctcccaa tttttcagtt
    12361 gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat
    12421 ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt
    12481 tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca gttaactgcg
    12541 gtcaagatat ttcttgaatc aggcgcctta gaccgctcgg ccaaacaacc aattacttgt
    12601 tgagaaatag agtataatta tcctataaat ataacgtttt tgaacacaca tgaacaagga
    12661 agtacaggac aattgatttt gaagagaatg tggattttga tgtaattgtt gggattccat
    12721 ttttaataag gcaataatat taggtatgta gatatactag aagttctcct cgaccggtcg
    12781 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
    12841 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
    12901 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
    12961 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
    13021 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
    13081 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
    13141 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
    13201 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
    13261 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
    13321 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    13381 gcaaggcgat taagttgggt
//
