LOCUS       sequence-181060-       13203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 13203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3487 (YCp LEU2 Rpb1 S1493A)
FEATURES             Location/Qualifiers
     source          1..13203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(187..775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(267..286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(946..1737,1738..1806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1569..1588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1807..1911)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1948..2451
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2493..2511
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2549..2571)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2671..2690
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3184..4278)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4279..4686)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(4987..5442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5124..5145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5336..5355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5568..5590
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5582..5599
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5583..5599
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5606..5625
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5606..5624
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(12705..12723)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12744..12760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12744..12760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12757..12779)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12768..12784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12792..12798,12799..12816,12817..12822))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12837..12858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
       61 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      121 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      181 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      241 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      301 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      361 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      421 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      481 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      541 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      601 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      661 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      721 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      781 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      841 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      901 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      961 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1021 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1081 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1141 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1201 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1261 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1321 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1381 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1441 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1501 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1561 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1621 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1681 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1741 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1801 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1861 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1921 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     1981 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     2041 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     2101 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     2161 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     2221 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     2281 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     2341 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     2401 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag
     2461 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2521 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2581 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     2641 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     2701 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
     2761 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
     2821 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
     2881 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
     2941 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
     3001 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
     3061 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
     3121 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
     3181 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
     3241 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
     3301 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
     3361 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
     3421 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
     3481 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     3541 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     3601 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     3661 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     3721 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     3781 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     3841 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     3901 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     3961 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     4021 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     4081 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     4141 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     4201 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     4261 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
     4321 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     4381 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     4441 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     4501 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     4561 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     4621 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     4681 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
     4741 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
     4801 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
     4861 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
     4921 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4981 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     5041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     5101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     5161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     5221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     5281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     5341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     5401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     5461 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5521 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5581 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctctt
     5641 gggaagcttc agtaccgcga tttttcagct aatggatcag atgatatata aacaaaaagt
     5701 gggatacagc atatcgacgc ctccactaca aattaacaga aggatgaaaa ccaataagaa
     5761 aatttctaaa agaagaagcc tgaaaaatct ccatggtgcc ttaaaaggac tattgaaaga
     5821 gtccggcaag aagagtgagt ccaaaatacg aaaacatagc gattgcaatc ctgttcatcg
     5881 agtatatccg ccaaatatcg aaaaacggaa gaccaaaaag gacgatggaa tttcacggcc
     5941 aattgctgaa agaaatggtc atgtgtatat aatgtccaag gaaaaccaca tcatccctaa
     6001 actaaccgat gatgaagtca tggagcgcca caaactagca gacgaaaaca tgagaaaagt
     6061 gtggtccaac atcataagca aatacgagtc tattgaagaa caaggagacc tcgtagacct
     6121 gaaaactggt gaaatagttg aagataatgg gcatatcaag acgctgacgg ccaacaacag
     6181 cacgaaagat aaaagaacaa aatacacaag cgtgctcaga gacattatcg acatcagcga
     6241 cgaggaagat ggtgataaaa atgatgaata tactctttgg gctaacgata gcgaggcaag
     6301 cgattcagag gtggatgctg acaacgacac tgaagaggaa aaagacgaga aattgataga
     6361 cgcagatttc aagaagtacg aggccaaact ctcgaaaagg atattacgag attaaggtgc
     6421 taaaatgccg tatattcgaa tttcattacc atcagaatat atatatatat atatgagtct
     6481 atattaggga ctatcatacg gtaacccgga cctccattac ccgcatgact ttactcacta
     6541 aagacgattg tcggttttaa acattttttt tttttcattt cgaccgtcgg ttctccttag
     6601 tttgttcagt ggcaacaagt ggacaaacaa gaagagcgtg cagcaccatt caccgccagc
     6661 tttgtatcgt cctttagagt gcagcaagaa cattaattgc tctcacagag gtatcataag
     6721 aacatccgat tcgttcatta actgaaatca tatctgtgtt gttctgatcg actctctttg
     6781 ttagttttat cttccatttt tttttattcc tttcattttc caataaaatt tgaacaggat
     6841 cgatataaac agataaaaac cctccaaagt taaccatttg tttatatttg cttttctgca
     6901 taagttttcc tagcgaattc cctgatcaac tttcaaggaa aactaaaact actgtattat
     6961 aagaggtttt ttttttcatt ccagattaat tttgaaatac gatatcctca agtttatcta
     7021 ccagaatatt tgactaagaa atcaaactct gttaataata atataattat aaaaacctca
     7081 actagaaact ccaaaaaaaa aaatttacca ttttttactt tctatccttg ttaaccaaat
     7141 ttcaaaaaaa ttttaccttt tctttttcca gaagagggac caatcataaa gatagcaata
     7201 acactttacc ccaaaatata aatcagacat ggtaggacaa cagtattcta gtgctccact
     7261 ccgtacagta aaagaggtcc aattcggtct tttctcacct gaagaagtta gagcaatcag
     7321 tgtggccaaa attagatttc cagagacaat ggatgaaacc cagacgagag cgaaaattgg
     7381 tggtctaaac gaccctaggt taggctctat tgatcgtaat ctgaagtgtc aaacttgtca
     7441 agagggtatg aacgaatgtc ctggtcattt tggtcacata gatttagcaa aacctgtatt
     7501 tcatgttggt tttattgcca aaattaagaa agtatgtgag tgtgtctgta tgcactgtgg
     7561 taagctatta ctggatgaac ataatgaatt aatgagacaa gctctagcaa tcaaagacag
     7621 taaaaaaagg tttgctgcaa tttggacttt atgtaaaaca aaaatggtct gcgaaacaga
     7681 tgtcccttct gaagatgatc ctactcagct cgtatcaagg ggaggttgtg gtaatacaca
     7741 gcctacaatt cgtaaggatg ggttgaaatt agttggtagt tggaaaaaag atagagccac
     7801 gggggatgcg gatgaaccag aactaagagt tttaagtacg gaggaaatct tgaatatttt
     7861 taagcatatc tcagtaaaag acttcactag tttgggtttc aacgaagttt tttctcgtcc
     7921 agaatggatg attttaacat gccttcctgt cccaccacca ccggtgcgtc catccatttc
     7981 cttcaatgaa tctcaaagag gtgaggatga tttaaccttt aaacttgctg atattttaaa
     8041 agctaatatt agtttggaaa cactagagca taacggtgct ccacatcatg ctattgaaga
     8101 agcagagagt ttattacaat ttcatgttgc cacttatatg gataatgata ttgctggtca
     8161 accacaagct ctgcagaagt ccggccgtcc cgttaaatct attcgtgctc gtttgaaggg
     8221 taaagagggt cgtatcagag gtaatttaat gggtaagcgt gtggattttt cggcaagaac
     8281 tgttatttct ggtgatccta atttggaatt agaccaagtc ggtgttccaa aatctattgc
     8341 caagacttta acatacccag aagtggtcac accatataac atagatcgtc tgacgcaact
     8401 tgttaggaat ggaccaaatg agcaccccgg tgccaaatac gtcattcgtg atagcggaga
     8461 ccgtatagat ttaagataca gtaaaagggc aggtgatatt caattacagt atgggtggaa
     8521 agttgaacgt catattatgg acaatgatcc agttttattc aaccgtcaac cttcgttgca
     8581 caaaatgtcc atgatggccc acagagtaaa agttattcca tattctacat ttagattgaa
     8641 tttgtccgtt acatctccat acaatgccga tttcgacggt gacgaaatga atcttcacgt
     8701 tcctcagtct gaggaaacaa gggcggaact ttctcaatta tgtgctgttc ctctgcaaat
     8761 tgtttcacca caatctaaca aaccttgtat gggtattgtt caagatactt tgtgtggtat
     8821 tcgtaaactg acattaagag atacatttat agaacttgat caagttttga atatgcttta
     8881 ttgggttcca gattgggatg gtgttattcc gacacctgca attatcaagc ccaaaccttt
     8941 gtggtccggt aaacaaatct tgtctgtggc tatcccaaac ggtattcatt tacaacgttt
     9001 tgatgagggc actactctgc tttctccaaa ggataatggt atgcttatta ttgacggtca
     9061 aatcattttt ggtgtagtag agaaaaaaac cgttggatcc tccaatggtg gtttaattca
     9121 tgttgttacg agagaaaagg gacctcaagt ttgtgctaag ttgtttggta acatacagaa
     9181 agttgttaac ttttggttac tacataatgg gttttcaaca ggtattggtg ataccattgc
     9241 ggacggccca acaatgaggg aaattacaga gacaattgca gaggctaaaa agaaagtttt
     9301 ggatgttacg aaagaagccc aggcaaactt attgactgct aaacatggta tgactctccg
     9361 tgagtctttt gaggataacg ttgttcggtt cctaaatgaa gcaagagata aggcaggtcg
     9421 tttagctgaa gtcaatttga aagatttgaa caatgtgaaa caaatggtta tggcaggttc
     9481 caagggttca tttattaata tcgcgcaaat gtcagcttgt gtaggacagc aatctgttga
     9541 aggtaaacgt attgcttttg ggttcgttga tcgtacctta cctcatttct ctaaagatga
     9601 ttactcccca gagtctaaag gttttgttga gaactcatat ttgagaggtt tgaccccaca
     9661 agaatttttt ttccatgcaa tgggtggtcg tgaaggtctt atcgataccg ccgtcaaaac
     9721 agccgaaaca ggttatattc aacgtcgttt agtgaaagct ctagaagata tcatggttca
     9781 ttacgataac accacaagaa actcattggg taacgttatt cagtttattt atggtgaaga
     9841 tggtatggat gctgcgcata ttgaaaagca atcgctagat actattggtg gctccgatgc
     9901 agcttttgaa aagagataca gagttgattt attgaataca gaccataccc ttgatccctc
     9961 actattggaa tccggatctg agatacttgg cgatttgaaa cttcaagttc tcctggatga
    10021 agaatacaaa caattagtga aagatcgtaa atttttgagg gaagtttttg ttgatggtga
    10081 agcaaactgg ccattaccag tcaacataag acgtattatt caaaatgctc aacaaacttt
    10141 ccacatagat catacgaaac catctgattt aacaatcaaa gacatcgttc ttggtgtaaa
    10201 ggatttgcaa gaaaacttat tagtgttgcg tggtaagaat gaaattatac aaaatgccca
    10261 gcgagatgca gttacattgt tctgctgttt attacgttcc cgtttggcca cacgtagagt
    10321 tctacaagag tacagactaa caaaacaggc attcgattgg gtattaagta atatcgaggc
    10381 acaattcctc cgttctgttg ttcaccctgg tgaaatggtt ggtgttctag cagcccaatc
    10441 cattggtgaa ccagccacac aaatgaccct taacaccttc cattttgctg gtgttgcttc
    10501 caaaaaagtt acttctggtg tcccccgttt aaaggaaatt ttgaatgtgg ccaaaaacat
    10561 gaaaacccct tccttgactg tatacttaga gcccgggcat gctgccgatc aagaacaagc
    10621 gaagttgatc agatctgcta tcgagcatac cactttaaag agtgtcacta ttgcttcaga
    10681 aatttactat gatcctgatc cacgttccac agttattcca gaagatgaag aaattatcca
    10741 acttcatttc tcattattgg atgaagaggc tgaacaatct tttgaccaac aatcaccttg
    10801 gttattacgt ctggaactgg atcgtgcagc aatgaatgat aaagacttaa caatgggtca
    10861 ggttggtgaa agaatcaagc aaacattcaa aaatgatttg tttgttatct ggtctgaaga
    10921 caacgatgag aagttgatca tccgttgtcg tgttgttcgt ccaaagtcac tagatgctga
    10981 gactgaagca gaagaagatc atatgttgaa gaaaattgag aacacaatgt tagagaatat
    11041 tacattacgt ggtgtagaga acatcgagcg tgttgtcatg atgaaatatg accgtaaagt
    11101 accaagtcca actggtgaat acgttaagga acctgaatgg gtgttggaaa cagatggtgt
    11161 taacttatct gaagttatga ctgttcctgg tatcgaccca accagaatct ataccaactc
    11221 cttcattgat ataatggaag ttctaggtat tgaagctggt cgtgcagcct tgtataaaga
    11281 agtttacaat gttattgctt ctgatggttc gtatgttaac taccgtcata tggctttgtt
    11341 agtcgatgtt atgacaaccc aaggtggctt aacttctgtt actcgtcatg gtttcaacag
    11401 atcaaataca ggtgccttaa tgagatgttc atttgaagaa actgtcgaaa ttttgtttga
    11461 agctggtgct tcagccgaat tagatgattg tcgtggtgtt tcggaaaatg tcattcttgg
    11521 tcaaatggct ccaatcggta ccggtgcatt tgatgtgatg atcgatgagg agtcactggt
    11581 aaaatacatg ccagaacaaa aaataactga gattgaagac ggacaagatg gtggcgtcac
    11641 accatacagt aacgaaagtg gtttggtcaa tgcagatctt gacgttaaag atgagctaat
    11701 gtttgcacct ctggttgatt cgggttcaaa tgacgctatg gctggaggat ttacagcgta
    11761 cggtggtgct gattatggtg aagccacgtc tccatttggt gcttatggtg aagcacctac
    11821 atctcccgga tttggagtct cctcaccagg cttttctcca acttccccaa catactctcc
    11881 tacctctcca gcgtactcac caacatcacc atcgtactca ccaacatcac catcgtactc
    11941 gccaacatca ccatcgtact cacctacatc accatcgtat tcaccaacgt caccatcata
    12001 ttcgccaacg tcaccatcat attcgccaac gtcgccatcg tattctccaa cgtcaccatc
    12061 gtattcgcca acgtcgcctt cctactctcc cacgtcgcca agctacagcc ctacgtctcc
    12121 ttcttattct cctacatctc catcatactc tcctacgtca ccaagttaca gcccaacgtc
    12181 accaagttac agcccaacgt ctccagccta ttccccaaca tcaccaagtt atagtcctac
    12241 atcgccttca tactctccaa catcaccatc ctattcccca acatcacctt cttactctcc
    12301 cacctctcca aactatagcc ctacttcacc ttcttactcc ccaacatctc caggctacag
    12361 cccaggatct cctgcatatt ctccaaagca agacgaacaa aagcataatg aaaatgaaaa
    12421 ttccagatga tatagtatat catccttacg tatttgacgt tattacatta tatatagttt
    12481 ctcaaataat atttctagtt tatttttgta tcataataaa aacgtatacc aaatatacca
    12541 ttatttttca tagcattatg gtagggatag ggaatcaagt aactaattta tatccgcaga
    12601 gcattgggaa aaccaacggc gctagtaaat gcatttaaat tacgtccgtc caacttctaa
    12661 gcttaagggt ttctcgaggg ggggcccggt accagctttt gttcccttta gtgagggtta
    12721 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    13021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    13081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    13141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    13201 agg
//
