LOCUS       sequence-181064-       13545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3509 (YCp LEU2 Rpb1 T1471A)
FEATURES             Location/Qualifiers
     source          1..13545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(453..1041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(1212..2003,2004..2072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1835..1854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2073..2177)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2214..2717
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2759..2777
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2815..2837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2937..2956
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3450..4544)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4545..4952)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(5253..5708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5390..5411)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5602..5621
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5834..5856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5848..5865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5849..5865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5872..5891
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5872..5890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(12971..12989)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13010..13026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13010..13026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13023..13045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13034..13050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13058..13064,13065..13082,13083..13088))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13103..13124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
       61 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      121 tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
      181 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
      241 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt
     2221 gctataaaaa taattataat ttaaattttt taatataaat atataaatta aaaatagaaa
     2281 gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag
     2341 ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc
     2401 gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt
     2461 tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat
     2521 atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt
     2581 ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata
     2641 aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa
     2701 gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa
     2761 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     2821 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     2881 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     2941 atcagagcag attgtactga gagtgcacca tatcgactac gtcgttaagg ccgtttctga
     3001 cagagtaaaa ttcttgaggg aactttcacc attatgggaa atggttcaag aaggtattga
     3061 cttaaactcc atcaaatggt caggtcattg agtgtttttt atttgttgta tttttttttt
     3121 tttagagaaa atcctccaat atataaatta ggaatcatag tttcatgatt ttctgttaca
     3181 cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattcttt
     3241 ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tacatcctcc
     3301 tttttctcct tcttgataaa tgtatgtaga ttgcgtatat agtttcgtct accctatgaa
     3361 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg
     3421 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg
     3481 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct
     3541 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat
     3601 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt
     3661 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct
     3721 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct
     3781 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt
     3841 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta
     3901 gggaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa
     3961 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa
     4021 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg
     4081 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg
     4141 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga
     4201 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt
     4261 tttagtaaac cttgttcagg tctaacacta ccggtacccc atttaggacc acccacagca
     4321 cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg aagtggaaca
     4381 cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg
     4441 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca
     4501 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct
     4561 tgaaatatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac
     4621 gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag
     4681 tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc
     4741 ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg
     4801 cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc
     4861 cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt
     4921 gcatcttacg atacctgagt attcccacag ttaactgcgg tcaagatatt tcttgaatca
     4981 ggcgccttag accgctcggc caaacaacca attacttgtt gagaaataga gtataattat
     5041 cctataaata taacgttttt gaacacacat gaacaaggaa gtacaggaca attgattttg
     5101 aagagaatgt ggattttgat gtaattgttg ggattccatt tttaataagg caataatatt
     5161 aggtatgtag atatactaga agttctcctc gaccggtcga tatgcggtgt gaaataccgc
     5221 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     5281 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     5341 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     5401 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     5461 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     5521 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5581 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5641 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5701 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5761 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5821 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     5881 tcactatagg gcgaattgga gctcttggga agcttcagta ccgcgatttt tcagctaatg
     5941 gatcagatga tatataaaca aaaagtggga tacagcatat cgacgcctcc actacaaatt
     6001 aacagaagga tgaaaaccaa taagaaaatt tctaaaagaa gaagcctgaa aaatctccat
     6061 ggtgccttaa aaggactatt gaaagagtcc ggcaagaaga gtgagtccaa aatacgaaaa
     6121 catagcgatt gcaatcctgt tcatcgagta tatccgccaa atatcgaaaa acggaagacc
     6181 aaaaaggacg atggaatttc acggccaatt gctgaaagaa atggtcatgt gtatataatg
     6241 tccaaggaaa accacatcat ccctaaacta accgatgatg aagtcatgga gcgccacaaa
     6301 ctagcagacg aaaacatgag aaaagtgtgg tccaacatca taagcaaata cgagtctatt
     6361 gaagaacaag gagacctcgt agacctgaaa actggtgaaa tagttgaaga taatgggcat
     6421 atcaagacgc tgacggccaa caacagcacg aaagataaaa gaacaaaata cacaagcgtg
     6481 ctcagagaca ttatcgacat cagcgacgag gaagatggtg ataaaaatga tgaatatact
     6541 ctttgggcta acgatagcga ggcaagcgat tcagaggtgg atgctgacaa cgacactgaa
     6601 gaggaaaaag acgagaaatt gatagacgca gatttcaaga agtacgaggc caaactctcg
     6661 aaaaggatat tacgagatta aggtgctaaa atgccgtata ttcgaatttc attaccatca
     6721 gaatatatat atatatatat gagtctatat tagggactat catacggtaa cccggacctc
     6781 cattacccgc atgactttac tcactaaaga cgattgtcgg ttttaaacat tttttttttt
     6841 tcatttcgac cgtcggttct ccttagtttg ttcagtggca acaagtggac aaacaagaag
     6901 agcgtgcagc accattcacc gccagctttg tatcgtcctt tagagtgcag caagaacatt
     6961 aattgctctc acagaggtat cataagaaca tccgattcgt tcattaactg aaatcatatc
     7021 tgtgttgttc tgatcgactc tctttgttag ttttatcttc catttttttt tattcctttc
     7081 attttccaat aaaatttgaa caggatcgat ataaacagat aaaaaccctc caaagttaac
     7141 catttgttta tatttgcttt tctgcataag ttttcctagc gaattccctg atcaactttc
     7201 aaggaaaact aaaactactg tattataaga ggtttttttt ttcattccag attaattttg
     7261 aaatacgata tcctcaagtt tatctaccag aatatttgac taagaaatca aactctgtta
     7321 ataataatat aattataaaa acctcaacta gaaactccaa aaaaaaaaat ttaccatttt
     7381 ttactttcta tccttgttaa ccaaatttca aaaaaatttt accttttctt tttccagaag
     7441 agggaccaat cataaagata gcaataacac tttaccccaa aatataaatc agacatggta
     7501 ggacaacagt attctagtgc tccactccgt acagtaaaag aggtccaatt cggtcttttc
     7561 tcacctgaag aagttagagc aatcagtgtg gccaaaatta gatttccaga gacaatggat
     7621 gaaacccaga cgagagcgaa aattggtggt ctaaacgacc ctaggttagg ctctattgat
     7681 cgtaatctga agtgtcaaac ttgtcaagag ggtatgaacg aatgtcctgg tcattttggt
     7741 cacatagatt tagcaaaacc tgtatttcat gttggtttta ttgccaaaat taagaaagta
     7801 tgtgagtgtg tctgtatgca ctgtggtaag ctattactgg atgaacataa tgaattaatg
     7861 agacaagctc tagcaatcaa agacagtaaa aaaaggtttg ctgcaatttg gactttatgt
     7921 aaaacaaaaa tggtctgcga aacagatgtc ccttctgaag atgatcctac tcagctcgta
     7981 tcaaggggag gttgtggtaa tacacagcct acaattcgta aggatgggtt gaaattagtt
     8041 ggtagttgga aaaaagatag agccacgggg gatgcggatg aaccagaact aagagtttta
     8101 agtacggagg aaatcttgaa tatttttaag catatctcag taaaagactt cactagtttg
     8161 ggtttcaacg aagttttttc tcgtccagaa tggatgattt taacatgcct tcctgtccca
     8221 ccaccaccgg tgcgtccatc catttccttc aatgaatctc aaagaggtga ggatgattta
     8281 acctttaaac ttgctgatat tttaaaagct aatattagtt tggaaacact agagcataac
     8341 ggtgctccac atcatgctat tgaagaagca gagagtttat tacaatttca tgttgccact
     8401 tatatggata atgatattgc tggtcaacca caagctctgc agaagtccgg ccgtcccgtt
     8461 aaatctattc gtgctcgttt gaagggtaaa gagggtcgta tcagaggtaa tttaatgggt
     8521 aagcgtgtgg atttttcggc aagaactgtt atttctggtg atcctaattt ggaattagac
     8581 caagtcggtg ttccaaaatc tattgccaag actttaacat acccagaagt ggtcacacca
     8641 tataacatag atcgtctgac gcaacttgtt aggaatggac caaatgagca ccccggtgcc
     8701 aaatacgtca ttcgtgatag cggagaccgt atagatttaa gatacagtaa aagggcaggt
     8761 gatattcaat tacagtatgg gtggaaagtt gaacgtcata ttatggacaa tgatccagtt
     8821 ttattcaacc gtcaaccttc gttgcacaaa atgtccatga tggcccacag agtaaaagtt
     8881 attccatatt ctacatttag attgaatttg tccgttacat ctccatacaa tgccgatttc
     8941 gacggtgacg aaatgaatct tcacgttcct cagtctgagg aaacaagggc ggaactttct
     9001 caattatgtg ctgttcctct gcaaattgtt tcaccacaat ctaacaaacc ttgtatgggt
     9061 attgttcaag atactttgtg tggtattcgt aaactgacat taagagatac atttatagaa
     9121 cttgatcaag ttttgaatat gctttattgg gttccagatt gggatggtgt tattccgaca
     9181 cctgcaatta tcaagcccaa acctttgtgg tccggtaaac aaatcttgtc tgtggctatc
     9241 ccaaacggta ttcatttaca acgttttgat gagggcacta ctctgctttc tccaaaggat
     9301 aatggtatgc ttattattga cggtcaaatc atttttggtg tagtagagaa aaaaaccgtt
     9361 ggatcctcca atggtggttt aattcatgtt gttacgagag aaaagggacc tcaagtttgt
     9421 gctaagttgt ttggtaacat acagaaagtt gttaactttt ggttactaca taatgggttt
     9481 tcaacaggta ttggtgatac cattgcggac ggcccaacaa tgagggaaat tacagagaca
     9541 attgcagagg ctaaaaagaa agttttggat gttacgaaag aagcccaggc aaacttattg
     9601 actgctaaac atggtatgac tctccgtgag tcttttgagg ataacgttgt tcggttccta
     9661 aatgaagcaa gagataaggc aggtcgttta gctgaagtca atttgaaaga tttgaacaat
     9721 gtgaaacaaa tggttatggc aggttccaag ggttcattta ttaatatcgc gcaaatgtca
     9781 gcttgtgtag gacagcaatc tgttgaaggt aaacgtattg cttttgggtt cgttgatcgt
     9841 accttacctc atttctctaa agatgattac tccccagagt ctaaaggttt tgttgagaac
     9901 tcatatttga gaggtttgac cccacaagaa ttttttttcc atgcaatggg tggtcgtgaa
     9961 ggtcttatcg ataccgccgt caaaacagcc gaaacaggtt atattcaacg tcgtttagtg
    10021 aaagctctag aagatatcat ggttcattac gataacacca caagaaactc attgggtaac
    10081 gttattcagt ttatttatgg tgaagatggt atggatgctg cgcatattga aaagcaatcg
    10141 ctagatacta ttggtggctc cgatgcagct tttgaaaaga gatacagagt tgatttattg
    10201 aatacagacc atacccttga tccctcacta ttggaatccg gatctgagat acttggcgat
    10261 ttgaaacttc aagttctcct ggatgaagaa tacaaacaat tagtgaaaga tcgtaaattt
    10321 ttgagggaag tttttgttga tggtgaagca aactggccat taccagtcaa cataagacgt
    10381 attattcaaa atgctcaaca aactttccac atagatcata cgaaaccatc tgatttaaca
    10441 atcaaagaca tcgttcttgg tgtaaaggat ttgcaagaaa acttattagt gttgcgtggt
    10501 aagaatgaaa ttatacaaaa tgcccagcga gatgcagtta cattgttctg ctgtttatta
    10561 cgttcccgtt tggccacacg tagagttcta caagagtaca gactaacaaa acaggcattc
    10621 gattgggtat taagtaatat cgaggcacaa ttcctccgtt ctgttgttca ccctggtgaa
    10681 atggttggtg ttctagcagc ccaatccatt ggtgaaccag ccacacaaat gacccttaac
    10741 accttccatt ttgctggtgt tgcttccaaa aaagttactt ctggtgtccc ccgtttaaag
    10801 gaaattttga atgtggccaa aaacatgaaa accccttcct tgactgtata cttagagccc
    10861 gggcatgctg ccgatcaaga acaagcgaag ttgatcagat ctgctatcga gcataccact
    10921 ttaaagagtg tcactattgc ttcagaaatt tactatgatc ctgatccacg ttccacagtt
    10981 attccagaag atgaagaaat tatccaactt catttctcat tattggatga agaggctgaa
    11041 caatcttttg accaacaatc accttggtta ttacgtctgg aactggatcg tgcagcaatg
    11101 aatgataaag acttaacaat gggtcaggtt ggtgaaagaa tcaagcaaac attcaaaaat
    11161 gatttgtttg ttatctggtc tgaagacaac gatgagaagt tgatcatccg ttgtcgtgtt
    11221 gttcgtccaa agtcactaga tgctgagact gaagcagaag aagatcatat gttgaagaaa
    11281 attgagaaca caatgttaga gaatattaca ttacgtggtg tagagaacat cgagcgtgtt
    11341 gtcatgatga aatatgaccg taaagtacca agtccaactg gtgaatacgt taaggaacct
    11401 gaatgggtgt tggaaacaga tggtgttaac ttatctgaag ttatgactgt tcctggtatc
    11461 gacccaacca gaatctatac caactccttc attgatataa tggaagttct aggtattgaa
    11521 gctggtcgtg cagccttgta taaagaagtt tacaatgtta ttgcttctga tggttcgtat
    11581 gttaactacc gtcatatggc tttgttagtc gatgttatga caacccaagg tggcttaact
    11641 tctgttactc gtcatggttt caacagatca aatacaggtg ccttaatgag atgttcattt
    11701 gaagaaactg tcgaaatttt gtttgaagct ggtgcttcag ccgaattaga tgattgtcgt
    11761 ggtgtttcgg aaaatgtcat tcttggtcaa atggctccaa tcggtaccgg tgcatttgat
    11821 gtgatgatcg atgaggagtc actggtaaaa tacatgccag aacaaaaaat aactgagatt
    11881 gaagacggac aagatggtgg cgtcgcacca tacagtaacg aaagtggttt ggtcaatgca
    11941 gatcttgacg ttaaagatga gctaatgttt tcacctctgg ttgattcggg ttcaaatgac
    12001 gctatggctg gaggatttac agcgtacggt ggtgctgatt atggtgaagc cacgtctcca
    12061 tttggtgctt atggtgaagc acctacatct cccggatttg gagtctcctc accaggcttt
    12121 tctccaactt ccccaacata ctctcctacc tctccagcgt actcaccaac atcaccatcg
    12181 tactcaccaa catcaccatc gtactcgcca acatcaccat cgtactcacc tacatcacca
    12241 tcgtattcac caacgtcacc atcatattcg ccaacgtcac catcatattc gccaacgtcg
    12301 ccatcgtatt ctccaacgtc accatcgtat tcgccaacgt cgccttccta ctctcccacg
    12361 tcgccaagct acagccctac gtctccttct tattctccta catctccatc atactctcct
    12421 acgtcaccaa gttacagccc aacgtcacca agttacagcc caacgtctcc agcctattcc
    12481 ccaacatcac caagttatag tcctacatcg ccttcatact ctccaacatc accatcctat
    12541 tccccaacat caccttctta ctctcccacc tctccaaact atagccctac ttcaccttct
    12601 tactccccaa catctccagg ctacagccca ggatctcctg catattctcc aaagcaagac
    12661 gaacaaaagc ataatgaaaa tgaaaattcc agatgatata gtatatcatc cttacgtatt
    12721 tgacgttatt acattatata tagtttctca aataatattt ctagtttatt tttgtatcat
    12781 aataaaaacg tataccaaat ataccattat ttttcatagc attatggtag ggatagggaa
    12841 tcaagtaact aatttatatc cgcagagcat tgggaaaacc aacggcgcta gtaaatgcat
    12901 ttaaattacg tccgtccaac ttctaagctt aagggtttct cgaggggggg cccggtacca
    12961 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
    13021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    13081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    13141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    13201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    13261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    13321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    13381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    13441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    13501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccga
//
