LOCUS       sequence-181066-       13357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3511 (YCp LEU2 Rpb1 P1472A)
FEATURES             Location/Qualifiers
     source          1..13357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(268..372)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    409..912
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     954..972
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1010..1032)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1132..1151
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1645..2739)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2740..3147)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3448..3903)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3585..3606)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3797..3816
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4029..4051
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4043..4060
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4044..4060
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4067..4086
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4067..4085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(11166..11184)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11205..11221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11205..11221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11218..11240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11229..11245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11253..11259,11260..11277,11278..11283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11298..11319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11436..11453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11607..12195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11687..11706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12366..13157,13158..13226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13227..13331)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
       61 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      361 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
      421 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
      481 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
      541 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
      601 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
      661 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
      721 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
      781 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
      841 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
      901 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      961 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     1141 agcagattgt actgagagtg caccatatcg actacgtcgt taaggccgtt tctgacagag
     1201 taaaattctt gagggaactt tcaccattat gggaaatggt tcaagaaggt attgacttaa
     1261 actccatcaa atggtcaggt cattgagtgt tttttatttg ttgtattttt ttttttttag
     1321 agaaaatcct ccaatatata aattaggaat catagtttca tgattttctg ttacacctaa
     1381 ctttttgtgt ggtgccctcc tccttgtcaa tattaatgtt aaagtgcaat tctttttcct
     1441 tatcacgttg agccattagt atcaatttgc ttacctgtat tcctttacat cctccttttt
     1501 ctccttcttg ataaatgtat gtagattgcg tatatagttt cgtctaccct atgaacatat
     1561 tccattttgt aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa
     1621 atatcataaa aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc
     1681 atcaccgact tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc
     1741 caaaaccttt ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa
     1801 tttcaacatc attgcagcag acaagatagt ggcgataggg ttgaccttat tctttggcaa
     1861 atctggagca gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa
     1921 agaggccaag gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc
     1981 ggagatgata tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt
     2041 cttaactagg atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtagggaa
     2101 ttcgttcttg atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt
     2161 agctttatcc aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc
     2221 cattcttgtg attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc
     2281 atcaccatcg tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac
     2341 aacgaagtca gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc
     2401 ggatgcaaag ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag
     2461 taaaccttgt tcaggtctaa cactaccggt accccattta ggaccaccca cagcacctaa
     2521 caaaacggca tcagccttct tggaggcttc cagcgcctca tctggaagtg gaacacctgt
     2581 agcatcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg
     2641 aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg
     2701 gtcacctggc aaaacgacga tcttcttagg ggcagacatt agaatggtat atccttgaaa
     2761 tatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg
     2821 ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact tcaagtattg
     2881 tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg gttccggcgc
     2941 tctcaccttt cctttttctc ccaatttttc agttgaaaaa ggtatatgcg tcaggcgacc
     3001 tctgaaatta acaaaaaatt tccagtcatc gaatttgatt ctgtgcgata gcgcccctgt
     3061 gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc
     3121 ttacgatacc tgagtattcc cacagttaac tgcggtcaag atatttcttg aatcaggcgc
     3181 cttagaccgc tcggccaaac aaccaattac ttgttgagaa atagagtata attatcctat
     3241 aaatataacg tttttgaaca cacatgaaca aggaagtaca ggacaattga ttttgaagag
     3301 aatgtggatt ttgatgtaat tgttgggatt ccatttttaa taaggcaata atattaggta
     3361 tgtagatata ctagaagttc tcctcgaccg gtcgatatgc ggtgtgaaat accgcacaga
     3421 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     3481 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3541 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3601 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3661 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3721 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3781 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3841 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3901 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3961 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4021 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     4081 atagggcgaa ttggagctct tgggaagctt cagtaccgcg atttttcagc taatggatca
     4141 gatgatatat aaacaaaaag tgggatacag catatcgacg cctccactac aaattaacag
     4201 aaggatgaaa accaataaga aaatttctaa aagaagaagc ctgaaaaatc tccatggtgc
     4261 cttaaaagga ctattgaaag agtccggcaa gaagagtgag tccaaaatac gaaaacatag
     4321 cgattgcaat cctgttcatc gagtatatcc gccaaatatc gaaaaacgga agaccaaaaa
     4381 ggacgatgga atttcacggc caattgctga aagaaatggt catgtgtata taatgtccaa
     4441 ggaaaaccac atcatcccta aactaaccga tgatgaagtc atggagcgcc acaaactagc
     4501 agacgaaaac atgagaaaag tgtggtccaa catcataagc aaatacgagt ctattgaaga
     4561 acaaggagac ctcgtagacc tgaaaactgg tgaaatagtt gaagataatg ggcatatcaa
     4621 gacgctgacg gccaacaaca gcacgaaaga taaaagaaca aaatacacaa gcgtgctcag
     4681 agacattatc gacatcagcg acgaggaaga tggtgataaa aatgatgaat atactctttg
     4741 ggctaacgat agcgaggcaa gcgattcaga ggtggatgct gacaacgaca ctgaagagga
     4801 aaaagacgag aaattgatag acgcagattt caagaagtac gaggccaaac tctcgaaaag
     4861 gatattacga gattaaggtg ctaaaatgcc gtatattcga atttcattac catcagaata
     4921 tatatatata tatatgagtc tatattaggg actatcatac ggtaacccgg acctccatta
     4981 cccgcatgac tttactcact aaagacgatt gtcggtttta aacatttttt ttttttcatt
     5041 tcgaccgtcg gttctcctta gtttgttcag tggcaacaag tggacaaaca agaagagcgt
     5101 gcagcaccat tcaccgccag ctttgtatcg tcctttagag tgcagcaaga acattaattg
     5161 ctctcacaga ggtatcataa gaacatccga ttcgttcatt aactgaaatc atatctgtgt
     5221 tgttctgatc gactctcttt gttagtttta tcttccattt ttttttattc ctttcatttt
     5281 ccaataaaat ttgaacagga tcgatataaa cagataaaaa ccctccaaag ttaaccattt
     5341 gtttatattt gcttttctgc ataagttttc ctagcgaatt ccctgatcaa ctttcaagga
     5401 aaactaaaac tactgtatta taagaggttt tttttttcat tccagattaa ttttgaaata
     5461 cgatatcctc aagtttatct accagaatat ttgactaaga aatcaaactc tgttaataat
     5521 aatataatta taaaaacctc aactagaaac tccaaaaaaa aaaatttacc attttttact
     5581 ttctatcctt gttaaccaaa tttcaaaaaa attttacctt ttctttttcc agaagaggga
     5641 ccaatcataa agatagcaat aacactttac cccaaaatat aaatcagaca tggtaggaca
     5701 acagtattct agtgctccac tccgtacagt aaaagaggtc caattcggtc ttttctcacc
     5761 tgaagaagtt agagcaatca gtgtggccaa aattagattt ccagagacaa tggatgaaac
     5821 ccagacgaga gcgaaaattg gtggtctaaa cgaccctagg ttaggctcta ttgatcgtaa
     5881 tctgaagtgt caaacttgtc aagagggtat gaacgaatgt cctggtcatt ttggtcacat
     5941 agatttagca aaacctgtat ttcatgttgg ttttattgcc aaaattaaga aagtatgtga
     6001 gtgtgtctgt atgcactgtg gtaagctatt actggatgaa cataatgaat taatgagaca
     6061 agctctagca atcaaagaca gtaaaaaaag gtttgctgca atttggactt tatgtaaaac
     6121 aaaaatggtc tgcgaaacag atgtcccttc tgaagatgat cctactcagc tcgtatcaag
     6181 gggaggttgt ggtaatacac agcctacaat tcgtaaggat gggttgaaat tagttggtag
     6241 ttggaaaaaa gatagagcca cgggggatgc ggatgaacca gaactaagag ttttaagtac
     6301 ggaggaaatc ttgaatattt ttaagcatat ctcagtaaaa gacttcacta gtttgggttt
     6361 caacgaagtt ttttctcgtc cagaatggat gattttaaca tgccttcctg tcccaccacc
     6421 accggtgcgt ccatccattt ccttcaatga atctcaaaga ggtgaggatg atttaacctt
     6481 taaacttgct gatattttaa aagctaatat tagtttggaa acactagagc ataacggtgc
     6541 tccacatcat gctattgaag aagcagagag tttattacaa tttcatgttg ccacttatat
     6601 ggataatgat attgctggtc aaccacaagc tctgcagaag tccggccgtc ccgttaaatc
     6661 tattcgtgct cgtttgaagg gtaaagaggg tcgtatcaga ggtaatttaa tgggtaagcg
     6721 tgtggatttt tcggcaagaa ctgttatttc tggtgatcct aatttggaat tagaccaagt
     6781 cggtgttcca aaatctattg ccaagacttt aacataccca gaagtggtca caccatataa
     6841 catagatcgt ctgacgcaac ttgttaggaa tggaccaaat gagcaccccg gtgccaaata
     6901 cgtcattcgt gatagcggag accgtataga tttaagatac agtaaaaggg caggtgatat
     6961 tcaattacag tatgggtgga aagttgaacg tcatattatg gacaatgatc cagttttatt
     7021 caaccgtcaa ccttcgttgc acaaaatgtc catgatggcc cacagagtaa aagttattcc
     7081 atattctaca tttagattga atttgtccgt tacatctcca tacaatgccg atttcgacgg
     7141 tgacgaaatg aatcttcacg ttcctcagtc tgaggaaaca agggcggaac tttctcaatt
     7201 atgtgctgtt cctctgcaaa ttgtttcacc acaatctaac aaaccttgta tgggtattgt
     7261 tcaagatact ttgtgtggta ttcgtaaact gacattaaga gatacattta tagaacttga
     7321 tcaagttttg aatatgcttt attgggttcc agattgggat ggtgttattc cgacacctgc
     7381 aattatcaag cccaaacctt tgtggtccgg taaacaaatc ttgtctgtgg ctatcccaaa
     7441 cggtattcat ttacaacgtt ttgatgaggg cactactctg ctttctccaa aggataatgg
     7501 tatgcttatt attgacggtc aaatcatttt tggtgtagta gagaaaaaaa ccgttggatc
     7561 ctccaatggt ggtttaattc atgttgttac gagagaaaag ggacctcaag tttgtgctaa
     7621 gttgtttggt aacatacaga aagttgttaa cttttggtta ctacataatg ggttttcaac
     7681 aggtattggt gataccattg cggacggccc aacaatgagg gaaattacag agacaattgc
     7741 agaggctaaa aagaaagttt tggatgttac gaaagaagcc caggcaaact tattgactgc
     7801 taaacatggt atgactctcc gtgagtcttt tgaggataac gttgttcggt tcctaaatga
     7861 agcaagagat aaggcaggtc gtttagctga agtcaatttg aaagatttga acaatgtgaa
     7921 acaaatggtt atggcaggtt ccaagggttc atttattaat atcgcgcaaa tgtcagcttg
     7981 tgtaggacag caatctgttg aaggtaaacg tattgctttt gggttcgttg atcgtacctt
     8041 acctcatttc tctaaagatg attactcccc agagtctaaa ggttttgttg agaactcata
     8101 tttgagaggt ttgaccccac aagaattttt tttccatgca atgggtggtc gtgaaggtct
     8161 tatcgatacc gccgtcaaaa cagccgaaac aggttatatt caacgtcgtt tagtgaaagc
     8221 tctagaagat atcatggttc attacgataa caccacaaga aactcattgg gtaacgttat
     8281 tcagtttatt tatggtgaag atggtatgga tgctgcgcat attgaaaagc aatcgctaga
     8341 tactattggt ggctccgatg cagcttttga aaagagatac agagttgatt tattgaatac
     8401 agaccatacc cttgatccct cactattgga atccggatct gagatacttg gcgatttgaa
     8461 acttcaagtt ctcctggatg aagaatacaa acaattagtg aaagatcgta aatttttgag
     8521 ggaagttttt gttgatggtg aagcaaactg gccattacca gtcaacataa gacgtattat
     8581 tcaaaatgct caacaaactt tccacataga tcatacgaaa ccatctgatt taacaatcaa
     8641 agacatcgtt cttggtgtaa aggatttgca agaaaactta ttagtgttgc gtggtaagaa
     8701 tgaaattata caaaatgccc agcgagatgc agttacattg ttctgctgtt tattacgttc
     8761 ccgtttggcc acacgtagag ttctacaaga gtacagacta acaaaacagg cattcgattg
     8821 ggtattaagt aatatcgagg cacaattcct ccgttctgtt gttcaccctg gtgaaatggt
     8881 tggtgttcta gcagcccaat ccattggtga accagccaca caaatgaccc ttaacacctt
     8941 ccattttgct ggtgttgctt ccaaaaaagt tacttctggt gtcccccgtt taaaggaaat
     9001 tttgaatgtg gccaaaaaca tgaaaacccc ttccttgact gtatacttag agcccgggca
     9061 tgctgccgat caagaacaag cgaagttgat cagatctgct atcgagcata ccactttaaa
     9121 gagtgtcact attgcttcag aaatttacta tgatcctgat ccacgttcca cagttattcc
     9181 agaagatgaa gaaattatcc aacttcattt ctcattattg gatgaagagg ctgaacaatc
     9241 ttttgaccaa caatcacctt ggttattacg tctggaactg gatcgtgcag caatgaatga
     9301 taaagactta acaatgggtc aggttggtga aagaatcaag caaacattca aaaatgattt
     9361 gtttgttatc tggtctgaag acaacgatga gaagttgatc atccgttgtc gtgttgttcg
     9421 tccaaagtca ctagatgctg agactgaagc agaagaagat catatgttga agaaaattga
     9481 gaacacaatg ttagagaata ttacattacg tggtgtagag aacatcgagc gtgttgtcat
     9541 gatgaaatat gaccgtaaag taccaagtcc aactggtgaa tacgttaagg aacctgaatg
     9601 ggtgttggaa acagatggtg ttaacttatc tgaagttatg actgttcctg gtatcgaccc
     9661 aaccagaatc tataccaact ccttcattga tataatggaa gttctaggta ttgaagctgg
     9721 tcgtgcagcc ttgtataaag aagtttacaa tgttattgct tctgatggtt cgtatgttaa
     9781 ctaccgtcat atggctttgt tagtcgatgt tatgacaacc caaggtggct taacttctgt
     9841 tactcgtcat ggtttcaaca gatcaaatac aggtgcctta atgagatgtt catttgaaga
     9901 aactgtcgaa attttgtttg aagctggtgc ttcagccgaa ttagatgatt gtcgtggtgt
     9961 ttcggaaaat gtcattcttg gtcaaatggc tccaatcggt accggtgcat ttgatgtgat
    10021 gatcgatgag gagtcactgg taaaatacat gccagaacaa aaaataactg agattgaaga
    10081 cggacaagat ggtggcgtca cagcatacag taacgaaagt ggtttggtca atgcagatct
    10141 tgacgttaaa gatgagctaa tgttttcacc tctggttgat tcgggttcaa atgacgctat
    10201 ggctggagga tttacagcgt acggtggtgc tgattatggt gaagccacgt ctccatttgg
    10261 tgcttatggt gaagcaccta catctcccgg atttggagtc tcctcaccag gcttttctcc
    10321 aacttcccca acatactctc ctacctctcc agcgtactca ccaacatcac catcgtactc
    10381 accaacatca ccatcgtact cgccaacatc accatcgtac tcacctacat caccatcgta
    10441 ttcaccaacg tcaccatcat attcgccaac gtcaccatca tattcgccaa cgtcgccatc
    10501 gtattctcca acgtcaccat cgtattcgcc aacgtcgcct tcctactctc ccacgtcgcc
    10561 aagctacagc cctacgtctc cttcttattc tcctacatct ccatcatact ctcctacgtc
    10621 accaagttac agcccaacgt caccaagtta cagcccaacg tctccagcct attccccaac
    10681 atcaccaagt tatagtccta catcgccttc atactctcca acatcaccat cctattcccc
    10741 aacatcacct tcttactctc ccacctctcc aaactatagc cctacttcac cttcttactc
    10801 cccaacatct ccaggctaca gcccaggatc tcctgcatat tctccaaagc aagacgaaca
    10861 aaagcataat gaaaatgaaa attccagatg atatagtata tcatccttac gtatttgacg
    10921 ttattacatt atatatagtt tctcaaataa tatttctagt ttatttttgt atcataataa
    10981 aaacgtatac caaatatacc attatttttc atagcattat ggtagggata gggaatcaag
    11041 taactaattt atatccgcag agcattggga aaaccaacgg cgctagtaaa tgcatttaaa
    11101 ttacgtccgt ccaacttcta agcttaaggg tttctcgagg gggggcccgg taccagcttt
    11161 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
    11221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    11341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    11401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    11461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    12001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    12061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    13021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13321 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
