LOCUS       sequence-181074-       11674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with an internal PreScission 
            protease site and 10xHis tag for purification of the linker-CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3568 (YCp LEU2 Rpb1
            P1455_E1456InsGGGGGLEVL...
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(241..345)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    382..885
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     927..945
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(983..1005)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1105..1124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1618..2712)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2713..3120)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3421..3876)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3558..3579)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3770..3789
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4002..4024
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4016..4033
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4017..4033
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4040..4059
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4040..4058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10043..10066
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             10067..10096
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(11208..11226)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11247..11263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11247..11263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11260..11282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11271..11287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11295..11301,11302..11319,11320..11325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11340..11361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11478..11495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
       61 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
      121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
      181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
      241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
      301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      361 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
      421 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
      481 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
      541 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
      601 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
      661 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
      721 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
      781 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
      841 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca
      901 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
      961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     1081 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     1141 tcgactacgt cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa ctttcaccat
     1201 tatgggaaat ggttcaagaa ggtattgact taaactccat caaatggtca ggtcattgag
     1261 tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat ataaattagg
     1321 aatcatagtt tcatgatttt ctgttacacc taactttttg tgtggtgccc tcctccttgt
     1381 caatattaat gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt
     1441 tgcttacctg tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt
     1501 gcgtatatag tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgtttct
     1561 attatgaatt tcatttataa agtttatgta caaatatcat aaaaaaagag aatcttttta
     1621 agcaaggatt ttcttaactt cttcggcgac agcatcaccg acttcggtgg tactgttgga
     1681 accacctaaa tcaccagttc tgatacctgc atccaaaacc tttttaactg catcttcaat
     1741 ggccttacct tcttcaggca agttcaatga caatttcaac atcattgcag cagacaagat
     1801 agtggcgata gggttgacct tattctttgg caaatctgga gcagaaccgt ggcatggttc
     1861 gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc aaggacgcag atggcaacaa
     1921 acccaaggaa cctgggataa cggaggcttc atcggagatg atatcaccaa acatgttgct
     1981 ggtgattata ataccattta ggtgggttgg gttcttaact aggatcatgg cggcagaatc
     2041 aatcaattga tgttgaacct tcaatgtagg gaattcgttc ttgatggttt cctccacagt
     2101 ttttctccat aatcttgaag aggccaaaac attagcttta tccaaggacc aaataggcaa
     2161 tggtggctca tgttgtaggg ccatgaaagc ggccattctt gtgattcttt gcacttctgg
     2221 aacggtgtat tgttcactat cccaagcgac accatcacca tcgtcttcct ttctcttacc
     2281 aaagtaaata cctcccacta attctctgac aacaacgaag tcagtacctt tagcaaattg
     2341 tggcttgatt ggagataagt ctaaaagaga gtcggatgca aagttacatg gtcttaagtt
     2401 ggcgtacaat tgaagttctt tacggatttt tagtaaacct tgttcaggtc taacactacc
     2461 ggtaccccat ttaggaccac ccacagcacc taacaaaacg gcatcagcct tcttggaggc
     2521 ttccagcgcc tcatctggaa gtggaacacc tgtagcatcg atagcagcac caccaattaa
     2581 atgattttcg aaatcgaact tgacattgga acgaacatca gaaatagctt taagaacctt
     2641 aatggcttcg gctgtgattt cttgaccaac gtggtcacct ggcaaaacga cgatcttctt
     2701 aggggcagac attagaatgg tatatccttg aaatatatat atatatattg ctgaaatgta
     2761 aaaggtaaga aaagttagaa agtaagacga ttgctaacca cctattggaa aaaacaatag
     2821 gtccttaaat aatattgtca acttcaagta ttgtgatgca agcatttagt catgaacgct
     2881 tctctattct atatgaaaag ccggttccgg cgctctcacc tttccttttt ctcccaattt
     2941 ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc
     3001 atcgaatttg attctgtgcg atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa
     3061 taatggttgc taagagattc gaactcttgc atcttacgat acctgagtat tcccacagtt
     3121 aactgcggtc aagatatttc ttgaatcagg cgccttagac cgctcggcca aacaaccaat
     3181 tacttgttga gaaatagagt ataattatcc tataaatata acgtttttga acacacatga
     3241 acaaggaagt acaggacaat tgattttgaa gagaatgtgg attttgatgt aattgttggg
     3301 attccatttt taataaggca ataatattag gtatgtagat atactagaag ttctcctcga
     3361 ccggtcgata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3421 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3481 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3541 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3601 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3661 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3721 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3781 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3841 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     3901 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     3961 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4021 aacgacggcc agtgaattgt aatacgactc actatagggc gaattggagc tcttgggaag
     4081 cttcagtacc gcgatttttc agctaatgga tcagatgata tataaacaaa aagtgggata
     4141 cagcatatcg acgcctccac tacaaattaa cagaaggatg aaaaccaata agaaaatttc
     4201 taaaagaaga agcctgaaaa atctccatgg tgccttaaaa ggactattga aagagtccgg
     4261 caagaagagt gagtccaaaa tacgaaaaca tagcgattgc aatcctgttc atcgagtata
     4321 tccgccaaat atcgaaaaac ggaagaccaa aaaggacgat ggaatttcac ggccaattgc
     4381 tgaaagaaat ggtcatgtgt atataatgtc caaggaaaac cacatcatcc ctaaactaac
     4441 cgatgatgaa gtcatggagc gccacaaact agcagacgaa aacatgagaa aagtgtggtc
     4501 caacatcata agcaaatacg agtctattga agaacaagga gacctcgtag acctgaaaac
     4561 tggtgaaata gttgaagata atgggcatat caagacgctg acggccaaca acagcacgaa
     4621 agataaaaga acaaaataca caagcgtgct cagagacatt atcgacatca gcgacgagga
     4681 agatggtgat aaaaatgatg aatatactct ttgggctaac gatagcgagg caagcgattc
     4741 agaggtggat gctgacaacg acactgaaga ggaaaaagac gagaaattga tagacgcaga
     4801 tttcaagaag tacgaggcca aactctcgaa aaggatatta cgagattaag gtgctaaaat
     4861 gccgtatatt cgaatttcat taccatcaga atatatatat atatatatga gtctatatta
     4921 gggactatca tacggtaacc cggacctcca ttacccgcat gactttactc actaaagacg
     4981 attgtcggtt ttaaacattt tttttttttc atttcgaccg tcggttctcc ttagtttgtt
     5041 cagtggcaac aagtggacaa acaagaagag cgtgcagcac cattcaccgc cagctttgta
     5101 tcgtccttta gagtgcagca agaacattaa ttgctctcac agaggtatca taagaacatc
     5161 cgattcgttc attaactgaa atcatatctg tgttgttctg atcgactctc tttgttagtt
     5221 ttatcttcca ttttttttta ttcctttcat tttccaataa aatttgaaca ggatcgatat
     5281 aaacagataa aaaccctcca aagttaacca tttgtttata tttgcttttc tgcataagtt
     5341 ttcctagcga attccctgat caactttcaa ggaaaactaa aactactgta ttataagagg
     5401 tttttttttt cattccagat taattttgaa atacgatatc ctcaagttta tctaccagaa
     5461 tatttgacta agaaatcaaa ctctgttaat aataatataa ttataaaaac ctcaactaga
     5521 aactccaaaa aaaaaaattt accatttttt actttctatc cttgttaacc aaatttcaaa
     5581 aaaattttac cttttctttt tccagaagag ggaccaatca taaagatagc aataacactt
     5641 taccccaaaa tataaatcag acatggtagg acaacagtat tctagtgctc cactccgtac
     5701 agtaaaagag gtccaattcg gtcttttctc acctgaagaa gttagagcaa tcagtgtggc
     5761 caaaattaga tttccagaga caatggatga aacccagacg agagcgaaaa ttggtggtct
     5821 aaacgaccct aggttaggct ctattgatcg taatctgaag tgtcaaactt gtcaagaggg
     5881 tatgaacgaa tgtcctggtc attttggtca catagattta gcaaaacctg tatttcatgt
     5941 tggttttatt gccaaaatta agaaagtatg tgagtgtgtc tgtatgcact gtggtaagct
     6001 attactggat gaacataatg aattaatgag acaagctcta gcaatcaaag acagtaaaaa
     6061 aaggtttgct gcaatttgga ctttatgtaa aacaaaaatg gtctgcgaaa cagatgtccc
     6121 ttctgaagat gatcctactc agctcgtatc aaggggaggt tgtggtaata cacagcctac
     6181 aattcgtaag gatgggttga aattagttgg tagttggaaa aaagatagag ccacggggga
     6241 tgcggatgaa ccagaactaa gagttttaag tacggaggaa atcttgaata tttttaagca
     6301 tatctcagta aaagacttca ctagtttggg tttcaacgaa gttttttctc gtccagaatg
     6361 gatgatttta acatgccttc ctgtcccacc accaccggtg cgtccatcca tttccttcaa
     6421 tgaatctcaa agaggtgagg atgatttaac ctttaaactt gctgatattt taaaagctaa
     6481 tattagtttg gaaacactag agcataacgg tgctccacat catgctattg aagaagcaga
     6541 gagtttatta caatttcatg ttgccactta tatggataat gatattgctg gtcaaccaca
     6601 agctctgcag aagtccggcc gtcccgttaa atctattcgt gctcgtttga agggtaaaga
     6661 gggtcgtatc agaggtaatt taatgggtaa gcgtgtggat ttttcggcaa gaactgttat
     6721 ttctggtgat cctaatttgg aattagacca agtcggtgtt ccaaaatcta ttgccaagac
     6781 tttaacatac ccagaagtgg tcacaccata taacatagat cgtctgacgc aacttgttag
     6841 gaatggacca aatgagcacc ccggtgccaa atacgtcatt cgtgatagcg gagaccgtat
     6901 agatttaaga tacagtaaaa gggcaggtga tattcaatta cagtatgggt ggaaagttga
     6961 acgtcatatt atggacaatg atccagtttt attcaaccgt caaccttcgt tgcacaaaat
     7021 gtccatgatg gcccacagag taaaagttat tccatattct acatttagat tgaatttgtc
     7081 cgttacatct ccatacaatg ccgatttcga cggtgacgaa atgaatcttc acgttcctca
     7141 gtctgaggaa acaagggcgg aactttctca attatgtgct gttcctctgc aaattgtttc
     7201 accacaatct aacaaacctt gtatgggtat tgttcaagat actttgtgtg gtattcgtaa
     7261 actgacatta agagatacat ttatagaact tgatcaagtt ttgaatatgc tttattgggt
     7321 tccagattgg gatggtgtta ttccgacacc tgcaattatc aagcccaaac ctttgtggtc
     7381 cggtaaacaa atcttgtctg tggctatccc aaacggtatt catttacaac gttttgatga
     7441 gggcactact ctgctttctc caaaggataa tggtatgctt attattgacg gtcaaatcat
     7501 ttttggtgta gtagagaaaa aaaccgttgg atcctccaat ggtggtttaa ttcatgttgt
     7561 tacgagagaa aagggacctc aagtttgtgc taagttgttt ggtaacatac agaaagttgt
     7621 taacttttgg ttactacata atgggttttc aacaggtatt ggtgatacca ttgcggacgg
     7681 cccaacaatg agggaaatta cagagacaat tgcagaggct aaaaagaaag ttttggatgt
     7741 tacgaaagaa gcccaggcaa acttattgac tgctaaacat ggtatgactc tccgtgagtc
     7801 ttttgaggat aacgttgttc ggttcctaaa tgaagcaaga gataaggcag gtcgtttagc
     7861 tgaagtcaat ttgaaagatt tgaacaatgt gaaacaaatg gttatggcag gttccaaggg
     7921 ttcatttatt aatatcgcgc aaatgtcagc ttgtgtagga cagcaatctg ttgaaggtaa
     7981 acgtattgct tttgggttcg ttgatcgtac cttacctcat ttctctaaag atgattactc
     8041 cccagagtct aaaggttttg ttgagaactc atatttgaga ggtttgaccc cacaagaatt
     8101 ttttttccat gcaatgggtg gtcgtgaagg tcttatcgat accgccgtca aaacagccga
     8161 aacaggttat attcaacgtc gtttagtgaa agctctagaa gatatcatgg ttcattacga
     8221 taacaccaca agaaactcat tgggtaacgt tattcagttt atttatggtg aagatggtat
     8281 ggatgctgcg catattgaaa agcaatcgct agatactatt ggtggctccg atgcagcttt
     8341 tgaaaagaga tacagagttg atttattgaa tacagaccat acccttgatc cctcactatt
     8401 ggaatccgga tctgagatac ttggcgattt gaaacttcaa gttctcctgg atgaagaata
     8461 caaacaatta gtgaaagatc gtaaattttt gagggaagtt tttgttgatg gtgaagcaaa
     8521 ctggccatta ccagtcaaca taagacgtat tattcaaaat gctcaacaaa ctttccacat
     8581 agatcatacg aaaccatctg atttaacaat caaagacatc gttcttggtg taaaggattt
     8641 gcaagaaaac ttattagtgt tgcgtggtaa gaatgaaatt atacaaaatg cccagcgaga
     8701 tgcagttaca ttgttctgct gtttattacg ttcccgtttg gccacacgta gagttctaca
     8761 agagtacaga ctaacaaaac aggcattcga ttgggtatta agtaatatcg aggcacaatt
     8821 cctccgttct gttgttcacc ctggtgaaat ggttggtgtt ctagcagccc aatccattgg
     8881 tgaaccagcc acacaaatga cccttaacac cttccatttt gctggtgttg cttccaaaaa
     8941 agttacttct ggtgtccccc gtttaaagga aattttgaat gtggccaaaa acatgaaaac
     9001 cccttccttg actgtatact tagagcccgg gcatgctgcc gatcaagaac aagcgaagtt
     9061 gatcagatct gctatcgagc ataccacttt aaagagtgtc actattgctt cagaaattta
     9121 ctatgatcct gatccacgtt ccacagttat tccagaagat gaagaaatta tccaacttca
     9181 tttctcatta ttggatgaag aggctgaaca atcttttgac caacaatcac cttggttatt
     9241 acgtctggaa ctggatcgtg cagcaatgaa tgataaagac ttaacaatgg gtcaggttgg
     9301 tgaaagaatc aagcaaacat tcaaaaatga tttgtttgtt atctggtctg aagacaacga
     9361 tgagaagttg atcatccgtt gtcgtgttgt tcgtccaaag tcactagatg ctgagactga
     9421 agcagaagaa gatcatatgt tgaagaaaat tgagaacaca atgttagaga atattacatt
     9481 acgtggtgta gagaacatcg agcgtgttgt catgatgaaa tatgaccgta aagtaccaag
     9541 tccaactggt gaatacgtta aggaacctga atgggtgttg gaaacagatg gtgttaactt
     9601 atctgaagtt atgactgttc ctggtatcga cccaaccaga atctatacca actccttcat
     9661 tgatataatg gaagttctag gtattgaagc tggtcgtgca gccttgtata aagaagttta
     9721 caatgttatt gcttctgatg gttcgtatgt taactaccgt catatggctt tgttagtcga
     9781 tgttatgaca acccaaggtg gcttaacttc tgttactcgt catggtttca acagatcaaa
     9841 tacaggtgcc ttaatgagat gttcatttga agaaactgtc gaaattttgt ttgaagctgg
     9901 tgcttcagcc gaattagatg attgtcgtgg tgtttcggaa aatgtcattc ttggtcaaat
     9961 ggctccaatc ggtaccggtg catttgatgt gatgatcgat gaggagtcac tggtaaaata
    10021 catgccagga ggaggaggag gactagaagt tctgttccag gggccccacc accaccacca
    10081 ccatcatcat catcatgaac aaaaaataac tgagattgaa gacggacaag atggtggcgt
    10141 cacaccatac agtaacgaaa gtggtttggt caatgcagat cttgacgtta aagatgagct
    10201 aatgttttca cctctggttg attcgggttc aaaagacgct atggctggag gatttacagc
    10261 gtacggtggt gctgattatg gtgaagccac gtctccattt ggtgcttatg gtgaagcacc
    10321 tacatctccc ggatttggag tctcctcacc aggcttttct ccaacttccc caacatactc
    10381 tcctacctct ccagcgtact caccaacatc accatcgtac tcaccaacat caccatcgta
    10441 ctcgccaaca tcaccatcgt actcacctac atcaccatcg tattcaccaa cgtcaccatc
    10501 atattcgcca acgtcaccat catattcgcc aacgtcgcca tcgtattctc caacgtcacc
    10561 atcgtattcg ccaacgtcgc cttcctactc tcccacgtcg ccaagctaca gccctacgtc
    10621 tccttcttat tctcctacat ctccatcata ctctcctacg tcaccaagtt acagcccaac
    10681 gtcaccaagt tacagcccaa cgtctccagc ctattcccca acatcaccaa gttatagtcc
    10741 tacatcgcct tcatactctc caacatcacc atcctattcc ccaacatcac cttcttactc
    10801 tcccacctct ccaaactata gccctacttc accttcttac tccccaacat ctccaggcta
    10861 cagcccagga tctcctgcat attctccaaa gcaagacgaa caaaagcata atgaaaatga
    10921 aaattccaga tgatatagta tatcatcctt acgtatttga cgttattaca ttatatatag
    10981 tttctcaaat aatatttcta gtttattttt gtatcataat aaaaacgtat accaaatata
    11041 ccattatttt tcatagcatt atggtaggga tagggaatca agtaactaat ttatatccgc
    11101 agagcattgg gaaaaccaac ggcgctagta aatgcattta aattacgtcc gtccaacttc
    11161 taagcttaag ggtttctcga gggggggccc ggtaccagct tttgttccct ttagtgaggg
    11221 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11461 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11521 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11581 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11641 ctggcgtttt tccataggct ccgcccccct gacg
//
