LOCUS       sequence-181077-       10679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pUB-nSypHer (NLS:sypHer:NLS)
FEATURES             Location/Qualifiers
     source          1..10679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..1096
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1162..1356
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1592..1614)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1700..1840
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1755..1774
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1855..1872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2026..2614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2106..2125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2860..3651)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    4172..4196
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4289..4541)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4561..5112)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5154..5333)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    5755..5779
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5799..5819)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7251..7271)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(7295..7319)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8010..8026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8010..8026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8023..8045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8034..8050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8058..8064,8065..8082,8083..8088))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8103..8124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8289..8313
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9644..10273
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
       61 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
      121 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
      181 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
      241 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
      301 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
      361 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
      421 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
      481 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
      541 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
      601 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
      661 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
      721 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
      781 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
      841 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
      901 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
      961 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     1021 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     1081 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     1141 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     1201 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     1261 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     1321 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     1381 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     1441 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     1501 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     1561 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     1621 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     1681 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     1741 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     1801 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     1861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc
     2701 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     2761 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc
     2821 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt
     2881 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     2941 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     3001 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     3061 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     3121 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     3181 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     3241 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     3301 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     3361 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     3421 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     3481 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     3541 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     3601 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa
     3661 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3721 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     3781 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     3841 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     3901 acaggcttat gtccactggg ttcgtgcccg aattgatcac aggcagcaac gctctgtcat
     3961 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     4021 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     4081 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     4141 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     4201 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
     4261 aattgaatta tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
     4321 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     4381 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     4441 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     4501 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctaggggtca
     4561 tcagatttcg gtgacgggca ggaccggacg gggcggcacc ggcaggctga agtccagctg
     4621 ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg
     4681 gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac
     4741 agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt
     4801 ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca
     4861 gtcgtaggcg ttgcgtgcct tccagggacc cgcgtaggcg atgccggcga cctcgccgtc
     4921 cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc
     4981 ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac
     5041 gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg
     5101 ttctgggctc atggtagatc ccctcgatcg agttgagagt gaatatgaga ctctaattgg
     5161 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     5221 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     5281 ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg ttctgtcagt
     5341 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     5401 ttctccgctc atgatcgata attcgcggta cccggggatc ctctagaggg cccgacgtcg
     5461 catgcctgca ggtcactgga ttttggtttt aggaattaga aattttattg atagaagtat
     5521 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     5581 ataagaaccc taattccctt atctgggaac tactcacaca ttattctgga gaaaaataga
     5641 gagagataga tttgtagaga gagactggtg atttttgcgg actctagcat ggccgcttat
     5701 aaactagttg gatatctcga gtcgacacgc gtagatctcc gcggacgtcc catcaccact
     5761 ttgtacaaga aagctgggtc ggcgcgccca ccctttcaaa cctttctctt cttcttaggc
     5821 actgcttgct tcaaaacctt atcaaagtga ccatccatcc tggctcttat agcctctgct
     5881 aactgttcat accttgatct caatggtgaa cctggcctgt agaccaatcc aatggtcctt
     5941 cttggctctg gtttaatgca tggaagataa actacaccat ccctcttcct ctctggtggc
     6001 acggcaagtg ctggtaacaa agttattcca cttcctgcgg ctaccatgtt cctcaaggtt
     6061 tctagtgatg tagccctgaa gtgagtatcc tcgtcagcac cggcttcgaa gcaaaatccg
     6121 gttccgttgt actctagctt gtgtccaaga atatttccgt cctctttgaa tccgatacct
     6181 ttaagctcta ttctattcac caaagtatct ccctcaaact tcacttctgc cctagtttta
     6241 taattaccgt cgtctttgaa gaatatagtc ctctcttgca catatccctc aggcatagca
     6301 ctcttgaaga agtcatgttg cttcatatgg tcaggatatc ttgcgaagca ctttaaaccg
     6361 taacctaagg ttgttactaa tgttggccat gggacaggaa gttttccagt tgtacagatt
     6421 aatttcaatg ttagttttcc gtaggttgcg tcaccctcac cttctccgga gactgaaaat
     6481 ttgtgaccgt tcacgtcacc atcaagttct actaatattg ggacaactcc tgtgaacaac
     6541 tcttctccct tggaacctgt acctccacta cctccgtcta cattgtatag ctcatccata
     6601 ccaagagtaa taccggcagc ggttacgaac tcaagcaaca ccatgtgatc ccttttctca
     6661 tttgggtctt tggataggac agactgaaaa gataggtagt ggttatctgg aagtaaaaca
     6721 ggtccgtcac cgattggggt gttctgttga taatggtctg ctagttgtac ggaaccatct
     6781 tccacgttat gtctaatttt gaagttggcc tttataccat tcttctgttt gtcggccatg
     6841 atatagacgt tatctgaatt gtaaccggcg gacatagctt gatccctcaa ggagtgtcca
     6901 tcctcaagca ttagaagttt ctcaccggcc aaatctgcca ttggaacgca ttctctattg
     6961 gcccatgggt gatcttcata gattgctaaa agcataggtt catcgaaaag aggcacttct
     7021 atgaatgcct cggattcctt taccaaagct agtatgacac aatcaagttt accactatct
     7081 aactgggcca atagttgatg agtctgtgcc tcgtgaaggt acatttcaag tttagggaaa
     7141 gtttggtgta gcattgggat aatgtgagga agtaagtaag gtccaactgt tgggattaga
     7201 ccgatatgca atggtcctga catagtctca ccctgttgag aagccatttc aacctttctc
     7261 ttcttcttag gcatggtgaa gggggcggcc gcggagcctg cttttttgta caaacttgtg
     7321 atgggccctc tagaggatcc cgcactcgag tgttaatcag aaaaactcag attaatctac
     7381 aaattcgatc gcacaaacta gaaactaaca ccagatctag atagaaatca caaatcgaag
     7441 agtaattatt cgacaaaact caaattattt gaacaaatcg gatgatatct atgaaaccct
     7501 aatcgagaat taagatgata tctaacgatc aaacccagaa aatcgtcttc gatctaagat
     7561 taacagaatc taaaccaaag aacatatacg aaattgggat cgaacgaaaa caaaatcgaa
     7621 gattttgaga gaataaggaa cacagaaatt taccttgatc acggtagaga gaattgagag
     7681 aaagttttta agattttgag aaattgaaat ctgaattgtg aagaagaaga gctctttggg
     7741 tattgtttta tagaagaaga agaagaaaag acgaggacga ctaggtcacg agaaagctaa
     7801 ggcggtgaag caatagctaa taataaaatg acacgtgtat tgagcgttgt ttacacgcaa
     7861 agttgttttt ggctaattgc cttattttta ggttgaggaa aagtatttgt gctttgagtt
     7921 gataaacacg actcgtgtgt gccggctgca accactttga cgccgtttat tactgactcg
     7981 tcgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     8041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8221 attggctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     8281 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     8341 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     8401 caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt actttaaagt
     8461 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     8521 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     8581 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     8641 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     8701 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     8761 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8821 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8881 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8941 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     9001 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     9061 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     9121 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     9181 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     9241 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     9301 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     9361 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     9421 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     9481 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     9541 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     9601 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     9661 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     9721 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9781 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9841 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9901 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9961 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
    10021 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
    10081 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
    10141 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
    10201 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
    10261 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
    10321 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
    10381 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
    10441 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
    10501 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
    10561 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
    10621 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaa
//
