LOCUS       sequence-181106-       12983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with an internal PreScission 
            protease site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 12983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
FEATURES             Location/Qualifiers
     source          1..12983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..504)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(186..207)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     398..417
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     630..652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     644..661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     645..661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     668..687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6656..6679
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(7791..7809)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7830..7846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7830..7846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7843..7865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7878..7884,7885..7902,7903..7908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8061..8078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8232..8820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8312..8331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8991..9782,9783..9851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9614..9633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9852..9956)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9993..10496
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10538..10556
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10594..10616)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10716..10735
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(11229..12323)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(12324..12731)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
       61 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      121 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      181 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      241 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      301 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      361 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      421 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      481 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
      541 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      601 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      661 tgaattgtaa tacgactcac tatagggcga attggagctc ttgggaagct tcagtaccgc
      721 gatttttcag ctaatggatc agatgatata taaacaaaaa gtgggataca gcatatcgac
      781 gcctccacta caaattaaca gaaggatgaa aaccaataag aaaatttcta aaagaagaag
      841 cctgaaaaat ctccatggtg ccttaaaagg actattgaaa gagtccggca agaagagtga
      901 gtccaaaata cgaaaacata gcgattgcaa tcctgttcat cgagtatatc cgccaaatat
      961 cgaaaaacgg aagaccaaaa aggacgatgg aatttcacgg ccaattgctg aaagaaatgg
     1021 tcatgtgtat ataatgtcca aggaaaacca catcatccct aaactaaccg atgatgaagt
     1081 catggagcgc cacaaactag cagacgaaaa catgagaaaa gtgtggtcca acatcataag
     1141 caaatacgag tctattgaag aacaaggaga cctcgtagac ctgaaaactg gtgaaatagt
     1201 tgaagataat gggcatatca agacgctgac ggccaacaac agcacgaaag ataaaagaac
     1261 aaaatacaca agcgtgctca gagacattat cgacatcagc gacgaggaag atggtgataa
     1321 aaatgatgaa tatactcttt gggctaacga tagcgaggca agcgattcag aggtggatgc
     1381 tgacaacgac actgaagagg aaaaagacga gaaattgata gacgcagatt tcaagaagta
     1441 cgaggccaaa ctctcgaaaa ggatattacg agattaaggt gctaaaatgc cgtatattcg
     1501 aatttcatta ccatcagaat atatatatat atatatgagt ctatattagg gactatcata
     1561 cggtaacccg gacctccatt acccgcatga ctttactcac taaagacgat tgtcggtttt
     1621 aaacattttt tttttttcat ttcgaccgtc ggttctcctt agtttgttca gtggcaacaa
     1681 gtggacaaac aagaagagcg tgcagcacca ttcaccgcca gctttgtatc gtcctttaga
     1741 gtgcagcaag aacattaatt gctctcacag aggtatcata agaacatccg attcgttcat
     1801 taactgaaat catatctgtg ttgttctgat cgactctctt tgttagtttt atcttccatt
     1861 tttttttatt cctttcattt tccaataaaa tttgaacagg atcgatataa acagataaaa
     1921 accctccaaa gttaaccatt tgtttatatt tgcttttctg cataagtttt cctagcgaat
     1981 tccctgatca actttcaagg aaaactaaaa ctactgtatt ataagaggtt ttttttttca
     2041 ttccagatta attttgaaat acgatatcct caagtttatc taccagaata tttgactaag
     2101 aaatcaaact ctgttaataa taatataatt ataaaaacct caactagaaa ctccaaaaaa
     2161 aaaaatttac cattttttac tttctatcct tgttaaccaa atttcaaaaa aattttacct
     2221 tttctttttc cagaagaggg accaatcata aagatagcaa taacacttta ccccaaaata
     2281 taaatcagac atggtaggac aacagtattc tagtgctcca ctccgtacag taaaagaggt
     2341 ccaattcggt cttttctcac ctgaagaagt tagagcaatc agtgtggcca aaattagatt
     2401 tccagagaca atggatgaaa cccagacgag agcgaaaatt ggtggtctaa acgaccctag
     2461 gttaggctct attgatcgta atctgaagtg tcaaacttgt caagagggta tgaacgaatg
     2521 tcctggtcat tttggtcaca tagatttagc aaaacctgta tttcatgttg gttttattgc
     2581 caaaattaag aaagtatgtg agtgtgtctg tatgcactgt ggtaagctat tactggatga
     2641 acataatgaa ttaatgagac aagctctagc aatcaaagac agtaaaaaaa ggtttgctgc
     2701 aatttggact ttatgtaaaa caaaaatggt ctgcgaaaca gatgtccctt ctgaagatga
     2761 tcctactcag ctcgtatcaa ggggaggttg tggtaataca cagcctacaa ttcgtaagga
     2821 tgggttgaaa ttagttggta gttggaaaaa agatagagcc acgggggatg cggatgaacc
     2881 agaactaaga gttttaagta cggaggaaat cttgaatatt tttaagcata tctcagtaaa
     2941 agacttcact agtttgggtt tcaacgaagt tttttctcgt ccagaatgga tgattttaac
     3001 atgccttcct gtcccaccac caccggtgcg tccatccatt tccttcaatg aatctcaaag
     3061 aggtgaggat gatttaacct ttaaacttgc tgatatttta aaagctaata ttagtttgga
     3121 aacactagag cataacggtg ctccacatca tgctattgaa gaagcagaga gtttattaca
     3181 atttcatgtt gccacttata tggataatga tattgctggt caaccacaag ctctgcagaa
     3241 gtccggccgt cccgttaaat ctattcgtgc tcgtttgaag ggtaaagagg gtcgtatcag
     3301 aggtaattta atgggtaagc gtgtggattt ttcggcaaga actgttattt ctggtgatcc
     3361 taatttggaa ttagaccaag tcggtgttcc aaaatctatt gccaagactt taacataccc
     3421 agaagtggtc acaccatata acatagatcg tctgacgcaa cttgttagga atggaccaaa
     3481 tgagcacccc ggtgccaaat acgtcattcg tgatagcgga gaccgtatag atttaagata
     3541 cagtaaaagg gcaggtgata ttcaattaca gtatgggtgg aaagttgaac gtcatattat
     3601 ggacaatgat ccagttttat tcaaccgtca accttcgttg cacaaaatgt ccatgatggc
     3661 ccacagagta aaagttattc catattctac atttagattg aatttgtccg ttacatctcc
     3721 atacaatgcc gatttcgacg gtgacgaaat gaatcttcac gttcctcagt ctgaggaaac
     3781 aagggcggaa ctttctcaat tatgtgctgt tcctctgcaa attgtttcac cacaatctaa
     3841 caaaccttgt atgggtattg ttcaagatac tttgtgtggt attcgtaaac tgacattaag
     3901 agatacattt atagaacttg atcaagtttt gaatatgctt tattgggttc cagattggga
     3961 tggtgttatt ccgacacctg caattatcaa gcccaaacct ttgtggtccg gtaaacaaat
     4021 cttgtctgtg gctatcccaa acggtattca tttacaacgt tttgatgagg gcactactct
     4081 gctttctcca aaggataatg gtatgcttat tattgacggt caaatcattt ttggtgtagt
     4141 agagaaaaaa accgttggat cctccaatgg tggtttaatt catgttgtta cgagagaaaa
     4201 gggacctcaa gtttgtgcta agttgtttgg taacatacag aaagttgtta acttttggtt
     4261 actacataat gggttttcaa caggtattgg tgataccatt gcggacggcc caacaatgag
     4321 ggaaattaca gagacaattg cagaggctaa aaagaaagtt ttggatgtta cgaaagaagc
     4381 ccaggcaaac ttattgactg ctaaacatgg tatgactctc cgtgagtctt ttgaggataa
     4441 cgttgttcgg ttcctaaatg aagcaagaga taaggcaggt cgtttagctg aagtcaattt
     4501 gaaagatttg aacaatgtga aacaaatggt tatggcaggt tccaagggtt catttattaa
     4561 tatcgcgcaa atgtcagctt gtgtaggaca gcaatctgtt gaaggtaaac gtattgcttt
     4621 tgggttcgtt gatcgtacct tacctcattt ctctaaagat gattactccc cagagtctaa
     4681 aggttttgtt gagaactcat atttgagagg tttgacccca caagaatttt ttttccatgc
     4741 aatgggtggt cgtgaaggtc ttatcgatac cgccgtcaaa acagccgaaa caggttatat
     4801 tcaacgtcgt ttagtgaaag ctctagaaga tatcatggtt cattacgata acaccacaag
     4861 aaactcattg ggtaacgtta ttcagtttat ttatggtgaa gatggtatgg atgctgcgca
     4921 tattgaaaag caatcgctag atactattgg tggctccgat gcagcttttg aaaagagata
     4981 cagagttgat ttattgaata cagaccatac ccttgatccc tcactattgg aatccggatc
     5041 tgagatactt ggcgatttga aacttcaagt tctcctggat gaagaataca aacaattagt
     5101 gaaagatcgt aaatttttga gggaagtttt tgttgatggt gaagcaaact ggccattacc
     5161 agtcaacata agacgtatta ttcaaaatgc tcaacaaact ttccacatag atcatacgaa
     5221 accatctgat ttaacaatca aagacatcgt tcttggtgta aaggatttgc aagaaaactt
     5281 attagtgttg cgtggtaaga atgaaattat acaaaatgcc cagcgagatg cagttacatt
     5341 gttctgctgt ttattacgtt cccgtttggc cacacgtaga gttctacaag agtacagact
     5401 aacaaaacag gcattcgatt gggtattaag taatatcgag gcacaattcc tccgttctgt
     5461 tgttcaccct ggtgaaatgg ttggtgttct agcagcccaa tccattggtg aaccagccac
     5521 acaaatgacc cttaacacct tccattttgc tggtgttgct tccaaaaaag ttacttctgg
     5581 tgtcccccgt ttaaaggaaa ttttgaatgt ggccaaaaac atgaaaaccc cttccttgac
     5641 tgtatactta gagcccgggc atgctgccga tcaagaacaa gcgaagttga tcagatctgc
     5701 tatcgagcat accactttaa agagtgtcac tattgcttca gaaatttact atgatcctga
     5761 tccacgttcc acagttattc cagaagatga agaaattatc caacttcatt tctcattatt
     5821 ggatgaagag gctgaacaat cttttgacca acaatcacct tggttattac gtctggaact
     5881 ggatcgtgca gcaatgaatg ataaagactt aacaatgggt caggttggtg aaagaatcaa
     5941 gcaaacattc aaaaatgatt tgtttgttat ctggtctgaa gacaacgatg agaagttgat
     6001 catccgttgt cgtgttgttc gtccaaagtc actagatgct gagactgaag cagaagaaga
     6061 tcatatgttg aagaaaattg agaacacaat gttagagaat attacattac gtggtgtaga
     6121 gaacatcgag cgtgttgtca tgatgaaata tgaccgtaaa gtaccaagtc caactggtga
     6181 atacgttaag gaacctgaat gggtgttgga aacagatggt gttaacttat ctgaagttat
     6241 gactgttcct ggtatcgacc caaccagaat ctataccaac tccttcattg atataatgga
     6301 agttctaggt attgaagctg gtcgtgcagc cttgtataaa gaagtttaca atgttattgc
     6361 ttctgatggt tcgtatgtta actaccgtca tatggctttg ttagtcgatg ttatgacaac
     6421 ccaaggtggc ttaacttctg ttactcgtca tggtttcaac agatcaaata caggtgcctt
     6481 aatgagatgt tcatttgaag aaactgtcga aattttgttt gaagctggtg cttcagccga
     6541 attagatgat tgtcgtggtg tttcggaaaa tgtcattctt ggtcaaatgg ctccaatcgg
     6601 taccggtgca tttgatgtga tgatcgatga ggagtcactg gtaaaataca tgccactaga
     6661 agttctgttc caggggcccg aacaaaaaat aactgagatt gaagacggac aagatggtgg
     6721 cgtcacacca tacagtaacg aaagtggttt ggtcaatgca gatcttgacg ttaaagatga
     6781 gctaatgttt tcacctctgg ttgattcggg ttcaaatgac gctatggctg gaggatttac
     6841 agcgtacggt ggtgctgatt atggtgaagc cacgtctcca tttggtgctt atggtgaagc
     6901 acctacatct cccggatttg gagtctcctc accaggcttt tctccaactt ccccaacata
     6961 ctctcctacc tctccagcgt actcaccaac atcaccatcg tactcaccaa catcaccatc
     7021 gtactcgcca acatcaccat cgtactcacc tacatcacca tcgtattcac caacgtcacc
     7081 atcatattcg ccaacgtcac catcatattc gccaacgtcg ccatcgtatt ctccaacgtc
     7141 accatcgtat tcgccaacgt cgccttccta ctctcccacg tcgccaagct acagccctac
     7201 gtctccttct tattctccta catctccatc atactctcct acgtcaccaa gttacagccc
     7261 aacgtcacca agttacagcc caacgtctcc agcctattcc ccaacatcac caagttatag
     7321 tcctacatcg ccttcatact ctccaacatc accatcctat tccccaacat caccttctta
     7381 ctctcccacc tctccaaact atagccctac ttcaccttct tactccccaa catctccagg
     7441 ctacagccca ggatctcctg catattctcc aaagcaagac gaacaaaagc ataatgaaaa
     7501 tgaaaattcc agatgatata gtatatcatc cttacgtatt tgacgttatt acattatata
     7561 tagtttctca aataatattt ctagtttatt tttgtatcat aataaaaacg tataccaaat
     7621 ataccattat ttttcatagc attatggtag ggatagggaa tcaagtaact aatttatatc
     7681 cgcagagcat tgggaaaacc aacggcgcta gtaaatgcat ttaaattacg tccgtccaac
     7741 ttctaagctt aagggtttct cgaggggggg cccggtacca gcttttgttc cctttagtga
     7801 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9961 tttccccgaa aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt
    10021 taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa
    10081 aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta
    10141 ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt
    10201 gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt
    10261 atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt
    10321 gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt
    10381 tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc
    10441 caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc
    10501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10561 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10621 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10681 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    10741 agtgcaccat atcgactacg tcgttaaggc cgtttctgac agagtaaaat tcttgaggga
    10801 actttcacca ttatgggaaa tggttcaaga aggtattgac ttaaactcca tcaaatggtc
    10861 aggtcattga gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata
    10921 tataaattag gaatcatagt ttcatgattt tctgttacac ctaacttttt gtgtggtgcc
    10981 ctcctccttg tcaatattaa tgttaaagtg caattctttt tccttatcac gttgagccat
    11041 tagtatcaat ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat
    11101 gtatgtagat tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc
    11161 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
    11221 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
    11281 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
    11341 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
    11401 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
    11461 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
    11521 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
    11581 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
    11641 gcggcagaat caatcaattg atgttgaacc ttcaatgtag ggaattcgtt cttgatggtt
    11701 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
    11761 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
    11821 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
    11881 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
    11941 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
    12001 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
    12061 ctaacactac cggtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
    12121 ttcttggagg cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca
    12181 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
    12241 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
    12301 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatatatt
    12361 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
    12421 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
    12481 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt
    12541 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
    12601 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
    12661 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
    12721 ttcccacagt taactgcggt caagatattt cttgaatcag gcgccttaga ccgctcggcc
    12781 aaacaaccaa ttacttgttg agaaatagag tataattatc ctataaatat aacgtttttg
    12841 aacacacatg aacaaggaag tacaggacaa ttgattttga agagaatgtg gattttgatg
    12901 taattgttgg gattccattt ttaataaggc aataatatta ggtatgtaga tatactagaa
    12961 gttctcctcg accggtcgat atg
//
