LOCUS       sequence-181107-       12959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12959)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 12959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3488 (YCp LEU2 Rpb1 P1494A)
FEATURES             Location/Qualifiers
     source          1..12959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..504)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(186..207)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     398..417
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     630..652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     644..661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     645..661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     668..687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7767..7785)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7806..7822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7806..7822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7819..7841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7830..7846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7854..7860,7861..7878,7879..7884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7899..7920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8037..8054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8208..8796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8288..8307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8967..9758,9759..9827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9590..9609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9828..9932)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9969..10472
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10514..10532
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10570..10592)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10692..10711
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(11205..12299)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(12300..12707)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
       61 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      121 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      181 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      241 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      301 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      361 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      421 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      481 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
      541 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      601 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      661 tgaattgtaa tacgactcac tatagggcga attggagctc ttgggaagct tcagtaccgc
      721 gatttttcag ctaatggatc agatgatata taaacaaaaa gtgggataca gcatatcgac
      781 gcctccacta caaattaaca gaaggatgaa aaccaataag aaaatttcta aaagaagaag
      841 cctgaaaaat ctccatggtg ccttaaaagg actattgaaa gagtccggca agaagagtga
      901 gtccaaaata cgaaaacata gcgattgcaa tcctgttcat cgagtatatc cgccaaatat
      961 cgaaaaacgg aagaccaaaa aggacgatgg aatttcacgg ccaattgctg aaagaaatgg
     1021 tcatgtgtat ataatgtcca aggaaaacca catcatccct aaactaaccg atgatgaagt
     1081 catggagcgc cacaaactag cagacgaaaa catgagaaaa gtgtggtcca acatcataag
     1141 caaatacgag tctattgaag aacaaggaga cctcgtagac ctgaaaactg gtgaaatagt
     1201 tgaagataat gggcatatca agacgctgac ggccaacaac agcacgaaag ataaaagaac
     1261 aaaatacaca agcgtgctca gagacattat cgacatcagc gacgaggaag atggtgataa
     1321 aaatgatgaa tatactcttt gggctaacga tagcgaggca agcgattcag aggtggatgc
     1381 tgacaacgac actgaagagg aaaaagacga gaaattgata gacgcagatt tcaagaagta
     1441 cgaggccaaa ctctcgaaaa ggatattacg agattaaggt gctaaaatgc cgtatattcg
     1501 aatttcatta ccatcagaat atatatatat atatatgagt ctatattagg gactatcata
     1561 cggtaacccg gacctccatt acccgcatga ctttactcac taaagacgat tgtcggtttt
     1621 aaacattttt tttttttcat ttcgaccgtc ggttctcctt agtttgttca gtggcaacaa
     1681 gtggacaaac aagaagagcg tgcagcacca ttcaccgcca gctttgtatc gtcctttaga
     1741 gtgcagcaag aacattaatt gctctcacag aggtatcata agaacatccg attcgttcat
     1801 taactgaaat catatctgtg ttgttctgat cgactctctt tgttagtttt atcttccatt
     1861 tttttttatt cctttcattt tccaataaaa tttgaacagg atcgatataa acagataaaa
     1921 accctccaaa gttaaccatt tgtttatatt tgcttttctg cataagtttt cctagcgaat
     1981 tccctgatca actttcaagg aaaactaaaa ctactgtatt ataagaggtt ttttttttca
     2041 ttccagatta attttgaaat acgatatcct caagtttatc taccagaata tttgactaag
     2101 aaatcaaact ctgttaataa taatataatt ataaaaacct caactagaaa ctccaaaaaa
     2161 aaaaatttac cattttttac tttctatcct tgttaaccaa atttcaaaaa aattttacct
     2221 tttctttttc cagaagaggg accaatcata aagatagcaa taacacttta ccccaaaata
     2281 taaatcagac atggtaggac aacagtattc tagtgctcca ctccgtacag taaaagaggt
     2341 ccaattcggt cttttctcac ctgaagaagt tagagcaatc agtgtggcca aaattagatt
     2401 tccagagaca atggatgaaa cccagacgag agcgaaaatt ggtggtctaa acgaccctag
     2461 gttaggctct attgatcgta atctgaagtg tcaaacttgt caagagggta tgaacgaatg
     2521 tcctggtcat tttggtcaca tagatttagc aaaacctgta tttcatgttg gttttattgc
     2581 caaaattaag aaagtatgtg agtgtgtctg tatgcactgt ggtaagctat tactggatga
     2641 acataatgaa ttaatgagac aagctctagc aatcaaagac agtaaaaaaa ggtttgctgc
     2701 aatttggact ttatgtaaaa caaaaatggt ctgcgaaaca gatgtccctt ctgaagatga
     2761 tcctactcag ctcgtatcaa ggggaggttg tggtaataca cagcctacaa ttcgtaagga
     2821 tgggttgaaa ttagttggta gttggaaaaa agatagagcc acgggggatg cggatgaacc
     2881 agaactaaga gttttaagta cggaggaaat cttgaatatt tttaagcata tctcagtaaa
     2941 agacttcact agtttgggtt tcaacgaagt tttttctcgt ccagaatgga tgattttaac
     3001 atgccttcct gtcccaccac caccggtgcg tccatccatt tccttcaatg aatctcaaag
     3061 aggtgaggat gatttaacct ttaaacttgc tgatatttta aaagctaata ttagtttgga
     3121 aacactagag cataacggtg ctccacatca tgctattgaa gaagcagaga gtttattaca
     3181 atttcatgtt gccacttata tggataatga tattgctggt caaccacaag ctctgcagaa
     3241 gtccggccgt cccgttaaat ctattcgtgc tcgtttgaag ggtaaagagg gtcgtatcag
     3301 aggtaattta atgggtaagc gtgtggattt ttcggcaaga actgttattt ctggtgatcc
     3361 taatttggaa ttagaccaag tcggtgttcc aaaatctatt gccaagactt taacataccc
     3421 agaagtggtc acaccatata acatagatcg tctgacgcaa cttgttagga atggaccaaa
     3481 tgagcacccc ggtgccaaat acgtcattcg tgatagcgga gaccgtatag atttaagata
     3541 cagtaaaagg gcaggtgata ttcaattaca gtatgggtgg aaagttgaac gtcatattat
     3601 ggacaatgat ccagttttat tcaaccgtca accttcgttg cacaaaatgt ccatgatggc
     3661 ccacagagta aaagttattc catattctac atttagattg aatttgtccg ttacatctcc
     3721 atacaatgcc gatttcgacg gtgacgaaat gaatcttcac gttcctcagt ctgaggaaac
     3781 aagggcggaa ctttctcaat tatgtgctgt tcctctgcaa attgtttcac cacaatctaa
     3841 caaaccttgt atgggtattg ttcaagatac tttgtgtggt attcgtaaac tgacattaag
     3901 agatacattt atagaacttg atcaagtttt gaatatgctt tattgggttc cagattggga
     3961 tggtgttatt ccgacacctg caattatcaa gcccaaacct ttgtggtccg gtaaacaaat
     4021 cttgtctgtg gctatcccaa acggtattca tttacaacgt tttgatgagg gcactactct
     4081 gctttctcca aaggataatg gtatgcttat tattgacggt caaatcattt ttggtgtagt
     4141 agagaaaaaa accgttggat cctccaatgg tggtttaatt catgttgtta cgagagaaaa
     4201 gggacctcaa gtttgtgcta agttgtttgg taacatacag aaagttgtta acttttggtt
     4261 actacataat gggttttcaa caggtattgg tgataccatt gcggacggcc caacaatgag
     4321 ggaaattaca gagacaattg cagaggctaa aaagaaagtt ttggatgtta cgaaagaagc
     4381 ccaggcaaac ttattgactg ctaaacatgg tatgactctc cgtgagtctt ttgaggataa
     4441 cgttgttcgg ttcctaaatg aagcaagaga taaggcaggt cgtttagctg aagtcaattt
     4501 gaaagatttg aacaatgtga aacaaatggt tatggcaggt tccaagggtt catttattaa
     4561 tatcgcgcaa atgtcagctt gtgtaggaca gcaatctgtt gaaggtaaac gtattgcttt
     4621 tgggttcgtt gatcgtacct tacctcattt ctctaaagat gattactccc cagagtctaa
     4681 aggttttgtt gagaactcat atttgagagg tttgacccca caagaatttt ttttccatgc
     4741 aatgggtggt cgtgaaggtc ttatcgatac cgccgtcaaa acagccgaaa caggttatat
     4801 tcaacgtcgt ttagtgaaag ctctagaaga tatcatggtt cattacgata acaccacaag
     4861 aaactcattg ggtaacgtta ttcagtttat ttatggtgaa gatggtatgg atgctgcgca
     4921 tattgaaaag caatcgctag atactattgg tggctccgat gcagcttttg aaaagagata
     4981 cagagttgat ttattgaata cagaccatac ccttgatccc tcactattgg aatccggatc
     5041 tgagatactt ggcgatttga aacttcaagt tctcctggat gaagaataca aacaattagt
     5101 gaaagatcgt aaatttttga gggaagtttt tgttgatggt gaagcaaact ggccattacc
     5161 agtcaacata agacgtatta ttcaaaatgc tcaacaaact ttccacatag atcatacgaa
     5221 accatctgat ttaacaatca aagacatcgt tcttggtgta aaggatttgc aagaaaactt
     5281 attagtgttg cgtggtaaga atgaaattat acaaaatgcc cagcgagatg cagttacatt
     5341 gttctgctgt ttattacgtt cccgtttggc cacacgtaga gttctacaag agtacagact
     5401 aacaaaacag gcattcgatt gggtattaag taatatcgag gcacaattcc tccgttctgt
     5461 tgttcaccct ggtgaaatgg ttggtgttct agcagcccaa tccattggtg aaccagccac
     5521 acaaatgacc cttaacacct tccattttgc tggtgttgct tccaaaaaag ttacttctgg
     5581 tgtcccccgt ttaaaggaaa ttttgaatgt ggccaaaaac atgaaaaccc cttccttgac
     5641 tgtatactta gagcccgggc atgctgccga tcaagaacaa gcgaagttga tcagatctgc
     5701 tatcgagcat accactttaa agagtgtcac tattgcttca gaaatttact atgatcctga
     5761 tccacgttcc acagttattc cagaagatga agaaattatc caacttcatt tctcattatt
     5821 ggatgaagag gctgaacaat cttttgacca acaatcacct tggttattac gtctggaact
     5881 ggatcgtgca gcaatgaatg ataaagactt aacaatgggt caggttggtg aaagaatcaa
     5941 gcaaacattc aaaaatgatt tgtttgttat ctggtctgaa gacaacgatg agaagttgat
     6001 catccgttgt cgtgttgttc gtccaaagtc actagatgct gagactgaag cagaagaaga
     6061 tcatatgttg aagaaaattg agaacacaat gttagagaat attacattac gtggtgtaga
     6121 gaacatcgag cgtgttgtca tgatgaaata tgaccgtaaa gtaccaagtc caactggtga
     6181 atacgttaag gaacctgaat gggtgttgga aacagatggt gttaacttat ctgaagttat
     6241 gactgttcct ggtatcgacc caaccagaat ctataccaac tccttcattg atataatgga
     6301 agttctaggt attgaagctg gtcgtgcagc cttgtataaa gaagtttaca atgttattgc
     6361 ttctgatggt tcgtatgtta actaccgtca tatggctttg ttagtcgatg ttatgacaac
     6421 ccaaggtggc ttaacttctg ttactcgtca tggtttcaac agatcaaata caggtgcctt
     6481 aatgagatgt tcatttgaag aaactgtcga aattttgttt gaagctggtg cttcagccga
     6541 attagatgat tgtcgtggtg tttcggaaaa tgtcattctt ggtcaaatgg ctccaatcgg
     6601 taccggtgca tttgatgtga tgatcgatga ggagtcactg gtaaaataca tgccagaaca
     6661 aaaaataact gagattgaag acggacaaga tggtggcgtc acaccataca gtaacgaaag
     6721 tggtttggtc aatgcagatc ttgacgttaa agatgagcta atgttttcag cactggttga
     6781 ttcgggttca aatgacgcta tggctggagg atttacagcg tacggtggtg ctgattatgg
     6841 tgaagccacg tctccatttg gtgcttatgg tgaagcacct acatctcccg gatttggagt
     6901 ctcctcacca ggcttttctc caacttcccc aacatactct cctacctctc cagcgtactc
     6961 accaacatca ccatcgtact caccaacatc accatcgtac tcgccaacat caccatcgta
     7021 ctcacctaca tcaccatcgt attcaccaac gtcaccatca tattcgccaa cgtcaccatc
     7081 atattcgcca acgtcgccat cgtattctcc aacgtcacca tcgtattcgc caacgtcgcc
     7141 ttcctactct cccacgtcgc caagctacag ccctacgtct ccttcttatt ctcctacatc
     7201 tccatcatac tctcctacgt caccaagtta cagcccaacg tcaccaagtt acagcccaac
     7261 gtctccagcc tattccccaa catcaccaag ttatagtcct acatcgcctt catactctcc
     7321 aacatcacca tcctattccc caacatcacc ttcttactct cccacctctc caaactatag
     7381 ccctacttca ccttcttact ccccaacatc tccaggctac agcccaggat ctcctgcata
     7441 ttctccaaag caagacgaac aaaagcataa tgaaaatgaa aattccagat gatatagtat
     7501 atcatcctta cgtatttgac gttattacat tatatatagt ttctcaaata atatttctag
     7561 tttatttttg tatcataata aaaacgtata ccaaatatac cattattttt catagcatta
     7621 tggtagggat agggaatcaa gtaactaatt tatatccgca gagcattggg aaaaccaacg
     7681 gcgctagtaa atgcatttaa attacgtccg tccaacttct aagcttaagg gtttctcgag
     7741 ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     7801 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7861 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7921 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     9961 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
    10021 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
    10081 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
    10141 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
    10201 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
    10261 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
    10321 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
    10381 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
    10441 acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa gaaaccatta ttatcatgac
    10501 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10561 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10621 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10681 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatcg actacgtcgt
    10741 taaggccgtt tctgacagag taaaattctt gagggaactt tcaccattat gggaaatggt
    10801 tcaagaaggt attgacttaa actccatcaa atggtcaggt cattgagtgt tttttatttg
    10861 ttgtattttt ttttttttag agaaaatcct ccaatatata aattaggaat catagtttca
    10921 tgattttctg ttacacctaa ctttttgtgt ggtgccctcc tccttgtcaa tattaatgtt
    10981 aaagtgcaat tctttttcct tatcacgttg agccattagt atcaatttgc ttacctgtat
    11041 tcctttacat cctccttttt ctccttcttg ataaatgtat gtagattgcg tatatagttt
    11101 cgtctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt atgaatttca
    11161 tttataaagt ttatgtacaa atatcataaa aaaagagaat ctttttaagc aaggattttc
    11221 ttaacttctt cggcgacagc atcaccgact tcggtggtac tgttggaacc acctaaatca
    11281 ccagttctga tacctgcatc caaaaccttt ttaactgcat cttcaatggc cttaccttct
    11341 tcaggcaagt tcaatgacaa tttcaacatc attgcagcag acaagatagt ggcgataggg
    11401 ttgaccttat tctttggcaa atctggagca gaaccgtggc atggttcgta caaaccaaat
    11461 gcggtgttct tgtctggcaa agaggccaag gacgcagatg gcaacaaacc caaggaacct
    11521 gggataacgg aggcttcatc ggagatgata tcaccaaaca tgttgctggt gattataata
    11581 ccatttaggt gggttgggtt cttaactagg atcatggcgg cagaatcaat caattgatgt
    11641 tgaaccttca atgtagggaa ttcgttcttg atggtttcct ccacagtttt tctccataat
    11701 cttgaagagg ccaaaacatt agctttatcc aaggaccaaa taggcaatgg tggctcatgt
    11761 tgtagggcca tgaaagcggc cattcttgtg attctttgca cttctggaac ggtgtattgt
    11821 tcactatccc aagcgacacc atcaccatcg tcttcctttc tcttaccaaa gtaaatacct
    11881 cccactaatt ctctgacaac aacgaagtca gtacctttag caaattgtgg cttgattgga
    11941 gataagtcta aaagagagtc ggatgcaaag ttacatggtc ttaagttggc gtacaattga
    12001 agttctttac ggatttttag taaaccttgt tcaggtctaa cactaccggt accccattta
    12061 ggaccaccca cagcacctaa caaaacggca tcagccttct tggaggcttc cagcgcctca
    12121 tctggaagtg gaacacctgt agcatcgata gcagcaccac caattaaatg attttcgaaa
    12181 tcgaacttga cattggaacg aacatcagaa atagctttaa gaaccttaat ggcttcggct
    12241 gtgatttctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacatt
    12301 agaatggtat atccttgaaa tatatatata tatattgctg aaatgtaaaa ggtaagaaaa
    12361 gttagaaagt aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat
    12421 attgtcaact tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata
    12481 tgaaaagccg gttccggcgc tctcaccttt cctttttctc ccaatttttc agttgaaaaa
    12541 ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc gaatttgatt
    12601 ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa
    12661 gagattcgaa ctcttgcatc ttacgatacc tgagtattcc cacagttaac tgcggtcaag
    12721 atatttcttg aatcaggcgc cttagaccgc tcggccaaac aaccaattac ttgttgagaa
    12781 atagagtata attatcctat aaatataacg tttttgaaca cacatgaaca aggaagtaca
    12841 ggacaattga ttttgaagag aatgtggatt ttgatgtaat tgttgggatt ccatttttaa
    12901 taaggcaata atattaggta tgtagatata ctagaagttc tcctcgaccg gtcgatatg
//
