LOCUS       sequence-181136-        9107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG
  TITLE     Orai1 is an essential pore subunit of the CRAC channel.
  JOURNAL   Nature. 2006 Sep 14. 443(7108):230-3.
  PUBMED    16921383
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Sep 14. 443(7108):230-3."
COMMENT     Sequence Label: human STIM1 YFP
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..38)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     138..157
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        302..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        682..885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     836..856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1125..1466
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1533..1949
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1879..1901
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1917..1933
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      4068..4077
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4074..4076,4077..4079,4080..4793)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4119..4140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4380..4399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4727..4748
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4804..4827
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4851..4877
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4878..4904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5002..5501
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5037..5060)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5434..5453
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5522..6121
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5522..5541)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6018..6038
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6221..6735
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6897..6913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6921..6927,6928..6945,6946..6951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6966..6987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7104..7121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7275..7863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7355..7374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8034..8825,8826..8894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8657..8676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8895..8999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9067..9085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
       61 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      121 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      181 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      241 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcgcgt
      301 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      361 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      421 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      721 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      781 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      841 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      901 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc
      961 ccaataaagc ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc
     1021 tcctcagatt gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt
     1081 tggagacccc tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc
     1141 gtttcgtgtc tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc
     1201 gtctgtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1261 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1321 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1381 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1441 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1501 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1561 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1621 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1681 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1741 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1801 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1861 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1921 atccagccct cactccttct ctaggcgccg gaattagatc tctcgagatg gatgtatgcg
     1981 tccgtcttgc cctgtggctc ctctggggac tcctcctgca ccagggccag agcctcagcc
     2041 atagtcacag tgagaaggcg acaggaacca gctcgggggc caactctgag gagtccactg
     2101 cagcagagtt ttgccgaatt gacaagcccc tgtgtcacag tgaggatgag aaactcagct
     2161 tcgaggcagt ccgtaacatc cacaaactga tggacgatga tgccaatggt gatgtggatg
     2221 tggaagaaag tgatgagttc ctgagggaag acctcaatta ccatgaccca acagtgaaac
     2281 acagcacctt ccatggtgag gataagctca tcagcgtgga ggacctgtgg aaggcatgga
     2341 agtcatcaga agtatacaat tggaccgtgg atgaggtggt acagtggctg atcacatatg
     2401 tggagctgcc tcagtatgag gagaccttcc ggaagctgca gctcagtggc catgccatgc
     2461 caaggctggc tgtcaccaac accaccatga cagggactgt gctgaagatg acagaccgga
     2521 gtcatcggca gaagctgcag ctgaaggctc tggatacagt gctctttggg cctcctctct
     2581 tgactcgcca taatcacctc aaggacttca tgctggtggt gtctatcgtt attggtgtgg
     2641 gcggctgctg gtttgcctat atccagaacc gttactccaa ggagcacatg aagaagatga
     2701 tgaaggactt ggaggggtta caccgagctg agcagagtct gcatgacctt caggaaaggc
     2761 tgcacaaggc ccaggaggag caccgcacag tggaggtgga gaaggtccat ctggaaaaga
     2821 agctgcgcga tgagatcaac cttgctaagc aggaagccca gcggctgaag gagctgcggg
     2881 agggtactga gaatgagcgg agccgccaaa aatatgctga ggaggagttg gagcaggttc
     2941 gggaggcctt gaggaaagca gagaaggagc tagaatctca cagctcatgg tatgctccag
     3001 aggcccttca gaagtggctg cagctgacac atgaggtgga ggtgcaatat tacaacatca
     3061 agaagcaaaa tgctgagaag cagctgctgg tggccaagga gggggctgag aagataaaaa
     3121 agaagagaaa cacactcttt ggcaccttcc acgtggccca cagctcttcc ctggatgatg
     3181 tagatcataa aattctaaca gctaagcaag cactgagcga ggtgacagca gcattgcggg
     3241 agcgcctgca ccgctggcaa cagatcgaga tcctctgtgg cttccagatt gtcaacaacc
     3301 ctggcatcca ctcactggtg gctgccctca acatagaccc cagctggatg ggcagtacac
     3361 gccccaaccc tgctcacttc atcatgactg acgacgtgga tgacatggat gaggagattg
     3421 tgtctccctt gtccatgcag tcccctagcc tgcagagcag tgttcggcag cgcctgacgg
     3481 agccacagca tggcctggga tctcagaggg atttgaccca ttccgattcg gagtcctccc
     3541 tccacatgag tgaccgccag cgtgtggccc ccaaacctcc tcagatgagc cgtgctgcag
     3601 acgaggctct caatgccatg acttccaatg gcagccaccg gctgatcgag ggggtccacc
     3661 cagggtctct ggtggagaaa ctgcctgaca gccctgccct ggccaagaag gcattactgg
     3721 cgctgaacca tgggctggac aaggcccaca gcctgatgga gctgagcccc tcagccccac
     3781 ctggtggctc tccacatttg gattcttccc gttctcacag ccccagctcc ccagacccag
     3841 acacaccatc tccagttggg gacagccgag ccctgcaagc cagccgaaac acacgcattc
     3901 cccacctggc tggcaagaag gctgtggctg aggaggataa tggctctatt ggcgaggaaa
     3961 cagactccag cccaggccgg aagaagtttc ccctcaaaat ctttaagaag cctcttaaga
     4021 agcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
     4081 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4141 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4201 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4261 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4321 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4381 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4441 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4501 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4561 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4621 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4681 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4741 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     4801 gctgactaca aggacgacga tgacaagtga gaattgtcga acgggccgct tacccatacg
     4861 atgttccgga ttacgcttac ccatatgatg ttccggatta cgcttaacta gagtgagggt
     4921 ccccacctgg gacccttgag agtatcaggt ctcccacgtg ggagacaaga aatccctgtt
     4981 taatatttaa cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     5041 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     5101 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     5161 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     5221 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     5281 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     5341 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     5401 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5461 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     5521 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5581 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5641 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5701 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5761 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5821 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5881 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5941 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6001 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6061 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6121 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat
     6181 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     6241 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga
     6301 atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
     6361 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     6421 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     6481 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     6541 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     6601 ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     6661 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     6721 gcgggggtct ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg
     6781 agtcgaatat taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat
     6841 actcctgaaa tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt
     6901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9001 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9061 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcgg
//
