LOCUS       sequence-181142-        1966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB1 from PCR product with FS1 and FS2 (in with 
            BsaI, out with BbsI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB1_L_12_syn_BsaI
FEATURES             Location/Qualifiers
     source          1..1966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      77..108
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        109..211
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             212..1027
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(285..304)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1051..1078
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1090..1677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1170..1189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1717..1734
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1718..1734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agtcagcttg atgtcatagc tgtttcctga ggctcaatac tgaccattta aatcatacct
       61 gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt
      121 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      181 atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg
      241 cttgaagccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      301 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
      361 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
      421 caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
      481 tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt
      541 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
      601 gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc
      661 tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg
      721 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaactcttgc cattctcacc
      781 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
      841 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
      901 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
      961 atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt
     1021 tttctaatga ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt
     1081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag
     1141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1561 gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1681 gatcctttga ttttctaccg aagaaaggcc cacccgtgta aaacgacggc cagtttatct
     1741 agtcagcttg attctagctg atcgtggacc ggaaggtgag ccagtgagtt gattgcagtc
     1801 cagttacgct ggagtctgag gctcgtcctg aatgatatgc ggcctcgaat tcgaagacgc
     1861 ggagtgagac cggatccatc tcgagggtct cacatggcgt cttcgaattc gcgcgtgatc
     1921 ttacggcatt atacgtatga tcggtccacg atcagctaga ttatct
//
