LOCUS       sequence-181146-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_AB_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..73
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        338..442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(443..511,512..1303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(661..680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1529..2117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2018..2037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
       61 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      121 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      181 caacactagt cgcaaaaaac cccgcccctg acagggcggg gttttttcgc ggtctccgat
      241 cggaggcgtc ttcggatccc tcgaggaaga cgccgctccg gggagaccac cggtgccgct
      301 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      421 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      481 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      541 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      601 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      661 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      721 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      781 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      841 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      901 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      961 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1021 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1081 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1141 tggagccggt gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1201 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1261 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1321 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1381 gatccttttt gataatctca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1441 ggggtgtaga tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1501 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1561 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1621 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1681 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1741 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1801 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1861 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1921 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1981 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2041 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2101 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2161 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccg
//
