LOCUS       sequence-181148-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_CD_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     153..170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        435..539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(540..608,609..1400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(758..777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1626..2214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2115..2134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      181 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cactagtcgc aaaaaacccc
      301 gcccctgaca gggcggggtt ttttcgcggt ctccaattgg aggcgtcttc ggatccctcg
      361 aggaagacgc cgctagctgg agaccaccgg tgccgctaca gggcgcgtca ggtggcactt
      421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc
     1261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1501 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgtagatct catgaccaaa
     1561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaa
//
