LOCUS       sequence-181149-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_DE_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        211..315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(316..384,385..1176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(534..553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1402..1990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1891..1910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2144..2161
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacact
       61 agtcgcaaaa aaccccgccc ctgacagggc ggggtttttt cgcggtctcc agctggaggc
      121 gtcttcggat ccctcgagga agacgccgct ggagggagac caccggtgcc gctacagggc
      181 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      841 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      901 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      961 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1021 ggtgagcgtg gctctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1081 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1141 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1201 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1261 tttgataatc tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1321 agatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2041 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2101 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2161 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgatt
//
