LOCUS       sequence-181155-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_GH_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..52
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        317..421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(422..490,491..1282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(640..659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1508..2096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1997..2016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
       61 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      121 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacactagtc gcaaaaaacc
      181 ccgcccctga cagggcgggg ttttttcgcg gtctcccgct ggaggcgtct tcggatccct
      241 cgaggaagac gccgctcaac ggagaccacc ggtgccgcta cagggcgcgt caggtggcac
      301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      361 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      421 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      481 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      541 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      601 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      661 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      721 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      781 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      841 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      901 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      961 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1021 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1081 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc
     1141 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1201 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1261 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1321 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1381 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtagat ctcatgacca
     1441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagc
//
