LOCUS       sequence-181157-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_HI_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..52
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        316..420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(421..489,490..1281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(639..658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1507..2095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1996..2015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
       61 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      121 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacactagtc gcaaaaaacc
      181 ccgcccctga cagggcgggg ttttttcgcg gtctcccaac ggaggcgtct tcggatccct
      241 cgaggaagac gccgctggtg gagaccaccg gtgccgctac agggcgcgtc aggtggcact
      301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      541 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      601 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      661 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      721 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      781 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      841 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      901 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      961 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1021 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1081 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
     1141 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1201 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1261 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1321 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1381 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgtagatc tcatgaccaa
     1441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1621 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1741 ggctgctgtc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagc
//
