LOCUS       sequence-181163-        3185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA 
            and FSC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_12_syn_A_C
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..61
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    178..199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(214..219,220..237,238..244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     257..279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     276..292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     276..292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     311..330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        311..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(625..643)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(626..643)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(657..674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(657..673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(666..688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(814..1269)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(951..972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1163..1182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1296..1400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1401..1469,1470..2261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1619..1638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2487..3075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2976..2995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
       61 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      121 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      181 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      241 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      301 cgccaagctc taatacgact cactataggg aaagctcggt accacgcatg ctgcagacgc
      361 gttacgtatc ggatccagaa ttcgtgatga tcgaagacgc ggagcgcaaa aaaccccgcc
      421 cctgacaggg cggggttttt tcgcgatcgg agaccggatc cctcgagggt ctccaattca
      481 tggcgtcttc ccggtttttt cgcgatcgga gaccggatcc ctcgagggtc tccaattcat
      541 ggatctgaat tcgtcgacaa gcttctcgag cctaggctag ctctagacca cacgtgtggg
      601 ggcccgagct cgcggccgct gtattctata gtgtcaccta aatggccgca caattcactg
      661 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      721 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      781 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
      841 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      901 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      961 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1021 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1081 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1141 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1201 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1261 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1321 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1381 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1441 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1501 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1561 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1621 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1681 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1741 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1801 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1861 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1921 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1981 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2041 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2101 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2161 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2221 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2281 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2341 tcctttttga taatctcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2401 ggtgtagatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3181 gcctt
//
