LOCUS       sequence-181164-        3137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA 
            and FSC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_12_syn_A_D
FEATURES             Location/Qualifiers
     source          1..3137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    299..320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(335..340,341..358,359..365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    373..389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     378..400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     397..413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     397..413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     432..451
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        432..450
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(698..716)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(699..716)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(730..747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(730..746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(739..761)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(887..1342)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1024..1045)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1236..1255
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1369..1473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1474..1542,1543..2334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1692..1711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2560..3137,1..11)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3049..3068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
       61 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      181 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      421 acgccaagct ctaatacgac tcactatagg gaaagctcgg taccacgcat gctgcagacg
      481 cgttacgtat cggatccaga attcgtgatg atcgaagacg cggagcgcaa aaaaccccgc
      541 ccctgacagg gcggggtttt ttcgcgatcg gagaccggat ccctcgaggg tctccagctc
      601 atggcgtctt cccggatctg aattcgtcga caagcttctc gagcctaggc tagctctaga
      661 ccacacgtgt gggggcccga gctcgcggcc gctgtattct atagtgtcac ctaaatggcc
      721 gcacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      781 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      841 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt
      901 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      961 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1021 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1081 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1141 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1201 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1261 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1321 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1381 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1441 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1501 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1561 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1621 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1681 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1741 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1801 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1861 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1921 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1981 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2041 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2101 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2161 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2221 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2281 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2341 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2401 atctaggtga agatcctttt tgataatctc atgaacaata aaactgtctg cttacataaa
     2461 cagtaataca aggggtgtag atctcatgac caaaatccct taacgtgagt tttcgttcca
     2521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3121 ctcgtcaggg gggcgga
//
