LOCUS       sequence-181168-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA 
            and FSE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_12_syn_A_E
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..64
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     314..333
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        314..332
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(590..608)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(591..608)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(622..639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(622..638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(631..653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(779..1234)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(916..937)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1128..1147
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1261..1365
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1366..1434,1435..2226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1584..1603)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2452..3040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2941..2960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
       61 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      301 ttacgccaag ctctaatacg actcactata gggaaagctc ggtaccacgc atgctgcaga
      361 cgcgttacgt atcggatcca gaattcgtga tgtcttcgat cccgggaaga cgccatgctc
      421 cggagaccct cgagggatcc ggtctccgat cgcgaaaaaa ccccgccctg tcaggggcgg
      481 ggttttttgc gctccgcgtc ttcgatcatc tgaattcgtc gacaagcttc tcgagcctag
      541 gctagctcta gaccacacgt gtgggggccc gagctcgcgg ccgctgtatt ctatagtgtc
      601 acctaaatgg ccgcacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      661 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      721 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
      781 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
      841 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
      901 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
      961 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     1021 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     1081 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     1141 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     1201 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     1261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1381 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1441 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1501 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1561 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1621 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1681 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1741 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1801 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1861 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1921 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1981 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2041 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2101 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2161 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2221 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaacaa taaaactgtc
     2341 tgcttacata aacagtaata caaggggtgt agatctcatg accaaaatcc cttaacgtga
     2401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3121 tgattctgtg gataaccgta ttaccgc
//
