LOCUS       sequence-18118-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Zhou Z, Sim J, Griffith J, Reed R
  TITLE     Purification and electron microscopic visualization of functional 
            human spliceosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7.
  PUBMED    12215496
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Sep 17. 99(19):12203-7."
COMMENT     Sequence Label: MS2-MBP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(381..398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(381..397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(390..412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(576..593)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      746..832
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggccggggat ctcgagggta gggcttctaa ctttactcag ttcgttctcg tcgacaatgg
       61 cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat
      121 cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc
      181 gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt
      241 aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa
      301 ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc
      361 cggcatctac taagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      421 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      481 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcag
      541 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
      601 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
      661 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
      721 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      781 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
      841 agcggatttg aacgttgcga
//
