LOCUS       sequence-181180-        9018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV zinc finger donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAV-CAGGS-EGFP
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     320..342
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     404..423
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      452..461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(458..460,461..463,464..1177)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(458..460,461..463,464..1174)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(503..524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(764..783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1111..1132
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1267..1286)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1332..1387
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1386..1405)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1748..1764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1772..1788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1796..1802,1803..1820,1821..1826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1841..1862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1936..2772
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(2806..2825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2807..2825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2832..2849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2832..2848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2841..2863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2986..3288
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3167..3186
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(3691..3710)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4305..4324
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(4685..5476,5477..5545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5308..5327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5669..6257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6158..6177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6411..6428
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6545..6566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6581..6586,6587..6604,6605..6611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6619..6635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6643..6659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6680..6698
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6733..7536
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    7543..7568
                     /label=SA
                     /note="splice acceptor site"
     CDS             7592..7645
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7655..8254
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7655..7674)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8151..8171
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8286..8303)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8292..8516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8522..8901
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
       61 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      121 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      181 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
      241 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
      301 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
      361 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
      421 gtgctgtctc atcattttgg caaagaattc cgccaccatg gtgagcaagg gcgaggagct
      481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      601 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      661 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      781 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      841 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      901 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      961 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1021 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1081 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1141 cgggatcact ctcggcatgg acgagctgta caagtaaacg cgtgaattca ctcctcaggt
     1201 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     1261 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     1321 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     1381 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     1441 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     1501 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     1561 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     1621 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     1681 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     1741 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1801 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1861 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcg
     1921 acagtactaa gctttactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
     1981 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
     2041 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
     2101 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
     2161 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
     2221 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
     2281 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
     2341 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
     2401 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
     2461 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
     2521 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
     2581 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
     2641 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
     2701 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
     2761 tggctctgct ctaagggcga attcgcggcc gctaaattca attcgcccta tagtgagtcg
     2821 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2881 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2941 cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac
     3001 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     3061 acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     3121 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     3181 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     3241 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg
     3301 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
     3361 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     3421 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     3481 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     3541 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     3601 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3661 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3721 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3781 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     3841 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3901 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3961 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4021 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4081 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4141 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4201 gcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4261 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4321 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4381 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
     4441 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4501 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4561 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc
     4621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5221 tttctgtgac tggtgaatac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5581 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6421 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6481 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6541 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6601 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6661 atgattacgc caagctcaga attaaccctc actaaaggga ctagtcctgc aggtttaaac
     6721 gaattcgccc tttgctttct ctgaccagca ttctctcccc tgggcctgtg ccgctttctg
     6781 tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc tgccgcctcc
     6841 ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt tgctgcccaa
     6901 ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg
     6961 atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa ccccatgccg
     7021 tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat ctctcgtttc
     7081 ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc
     7141 tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg ctttagccac
     7201 ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat tcgggtcacc
     7261 tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt ctgtgctagc
     7321 tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta gtcttcttcc
     7381 tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc agggatcctg
     7441 tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct ctggttctgg
     7501 gtacttttat ctgtcccctc caccccacag tggggcaagc ttctgacctc ttctcttcct
     7561 cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc taacatgcgg
     7621 tgacgtggag gagaatcccg gccctaggct cgagatgacc gagtacaagc ccacggtgcg
     7681 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     7741 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7801 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7861 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7921 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7981 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8041 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8101 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8161 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8221 cacctggtgc atgacccgca agcccggtgc ctgatctaga gggcccgttt aaacccgctg
     8281 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8341 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8401 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     8461 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgggtct
     8521 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     8581 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8641 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8701 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8761 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8821 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8881 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     8941 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     9001 atgggggcgg gggggggg
//
