LOCUS       sequence-181202-       10222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lenti sgRNA cloning backbone with MS2 loops at 
            tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains 
            BsmBI sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti sgRNA(MS2)_puro backbone
FEATURES             Location/Qualifiers
     source          1..10222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..101)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        86..415
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      266..401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     328..347
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        463..510
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             529..903
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1033..1154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1070..1089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1124..1143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1203..1219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1203..1219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1216..1238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1227..1243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1251..1257,1258..1275,1276..1281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1296..1317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1434..1451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1605..2193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1685..1704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2364..3155,3156..3224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2987..3006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3225..3329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3404..3423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3595..3974
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3976..4174
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4129..4149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4192..4372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4419..4544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5037..5270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5455..5499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5797..5914
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5964..6212
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5964..5984
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6135..6154
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6250..6268
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6320..6338
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6462..7640
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6693..7631
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7588..7608
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7647..8246
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7647..7666)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8143..8163
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8271..8859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8324..8344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8742..8753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8862..8878)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8863..8879)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9384..9564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9590..9607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9596..9820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9866..10222,1..72)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9953..9972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10163..10184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
       61 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
      181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      241 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      301 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
      361 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
      421 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
      481 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
      541 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
      601 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
      661 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
      721 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
      781 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
      841 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
      901 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
      961 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     1021 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1141 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     1201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2041 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3361 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     3421 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     3481 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     3541 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     3601 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3661 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3721 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3781 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3841 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3901 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3961 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4021 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4081 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4141 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     4201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     4381 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     4441 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     4501 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     4561 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     4621 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     4681 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     4741 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     4801 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     4861 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     4921 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     4981 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     5041 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     5101 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     5161 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     5221 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     5281 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     5341 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     5401 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     5461 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     5521 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     5581 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     5641 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     5701 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     5761 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     5821 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5881 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     5941 tccagtttgg ttaattagct agcgagggcc tatttcccat gattccttca tatttgcata
     6001 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     6061 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     6121 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     6181 ctttatatat cttgtggaaa ggacgaaaca ccggagacgg gataccgtct ctgttttaga
     6241 gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa ataaggctag
     6301 tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa gtggcaccga
     6361 gtcggtgctt tttttggatc ctgcaaagat ggataaagtt ttaaacagag aggaatcttt
     6421 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     6481 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     6541 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     6601 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     6661 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     6721 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     6781 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     6841 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     6901 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     6961 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     7021 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     7081 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     7141 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     7201 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     7261 ggccgcttcc cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc
     7321 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     7381 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     7441 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     7501 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     7561 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     7621 ttccatttca ggtgtcgtga tgtacaatga ccgagtacaa gcccacggtg cgcctcgcca
     7681 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     7741 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7801 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7861 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7921 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7981 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc
     8041 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     8101 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     8161 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     8221 gcatgacccg caagcccggt gcctgagaat tcgatatcaa gcttatcggt aatcaacctc
     8281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     8341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8821 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     8881 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8941 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     9001 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9061 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     9121 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     9181 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     9241 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     9301 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     9361 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9541 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     9601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     9661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     9721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     9781 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     9841 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9961 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10021 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10081 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10201 atctcggtct attcttttga tt
//
