LOCUS       sequence-181208-       13661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of human Plexin-B2 with deletion of 
            C-terminal VTDL aminoacids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, 
            Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, 
            Dai G, Zou H, Friedel RH
  TITLE     Plexin-B2 facilitates glioblastoma infiltration by modulating cell 
            biomechanics.
  JOURNAL   Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
  PUBMED    33514835
REFERENCE   2  (bases 1 to 13661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-01-29"; volume: "4(1):145. doi"; pages: " 
            10.1038/s42003-021-01667-4"
COMMENT     Sequence Label: pLV-PLXNB2-dVTDL
FEATURES             Location/Qualifiers
     source          1..13661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(61..560)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(109..128)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     502..525
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    603..1191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             709..720
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1118..1138
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1222..1246
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6779..6803)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6858..7368)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6921..6939)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7414..7531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7798..7842)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8027..8260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8753..8878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8925..9105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9106..9332)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9360..9380)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9360..9378)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9399..9415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9399..9415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9412..9434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9423..9439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9447..9453,9454..9471,9472..9477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9492..9513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9630..9647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9801..10389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9881..9900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10560..11351,11352..11420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11183..11202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11421..11525)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11551..12006)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11688..11709)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11900..11919
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12133..12155
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12147..12164
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12148..12164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12174..12193
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12174..12192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      12213..12348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12388..12509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12399..12418)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12453..12472
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             12581..12814
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(12905..13661,1..47))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13016..13035)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13626..13645
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gccggagaac ctgcgtgcaa tccatcttgt tcaatggccg atcccatatt ggctgcaggt
       61 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
      121 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
      181 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
      241 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
      301 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
      361 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
      421 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
      481 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
      541 gaaaagcgcc tcccctaccc ggtagaatta gatcactagt ccggatatct gcagaattcc
      601 aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
      661 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
      721 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
      781 tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
      841 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
      901 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
      961 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     1021 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     1081 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     1141 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgagcgg
     1201 ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggcccat gaggccctac
     1261 ttgttctcca gtgcagcggc aatctgctgc aggcggaagg ccagctgcat cttctgggcg
     1321 gcaggatcct cctccaaggc attgatgatc tcgtcatagt acttctgcgt gtattggtag
     1381 agctggtgga gtgccacgag ggtgttcaag gagtccgtgt gcgcccggga aatctctgcc
     1441 aggtgtgtgt tcatgtcctg gtcgctgacc tgcaccatct gccggatccc cttgtagtaa
     1501 tcctccacca tcttcttgta ggtggagatc tccttggcgt acagcagctt gttgctggga
     1561 gaatcgcggc tcagcttatg ctccgtgcgc gtgcaggcat ccatgaaggt ctgcgcgatg
     1621 actgacagcg aggcgtccac cacctcgtgg acatgcacgt caaagatgaa gtgggggttc
     1681 ttgaggatgt tcacccagaa ccggagcggt aagctgttcg tcttccagat gtggatggtg
     1741 tcttcatcct ggatgttgtg cttctctgcc tgctcgtcca ggaagtcgaa gaagtacttg
     1801 actgcaggtg gcaccgcgtg cccaggcgcc agcacgctct ggaagaagtt gtccacaaac
     1861 tgctgcagtg tgcccttgac tgagagcagc cgcgtcaggt agatctcggt gatggccttc
     1921 gtccgctcct tctctttcac gctgcctctc ttggacttgc cctcgtccac ctcgtcggtc
     1981 ggccgcacca ggtgccacac ccggttctcc tcctccagga gggcatggcg ctccccaggc
     2041 aggtcctgct ggctgtcctc cggctgctgg gagaccccca ccttggacag gatgagggtg
     2101 gctccatccc ggacattgta gtgcataagg gtgttgacgc gcttccaccg gccctcccgc
     2161 tgtgacgtca ggtccaggtc cgacaggatc tgcgctgtgg agcccggacg ccactccagg
     2221 accacgctgt ctggcctggg ccagcaggag cagggctgcc cacggtacac ctggtcaatg
     2281 atcttctcct tgacctggga gatggtgtca cagttgagga ccttcaccgg gatggcgtcc
     2341 actccctcgt cctgcacgat cacgctcacc gtcaggggtg cgtactccac atcatccccc
     2401 agcagccccg tgtcgttgag agtgtacttg gccttcttct gtaccgcatc caccgggccc
     2461 ttttccacct gatgtttgat ggccttgaag agcttgtaca ggggctcccc ggcactgtcc
     2521 ttgaggtact ggtacaggca gatggacatc cagttggaca gcatcctctc caccacagtc
     2581 tcagacctgc gcagcatcag cttggggttc ttggccacca cgtactgctc caggagctcc
     2641 aggaagagcg tgtgcatgat gtccgtgtag tactccagtt tcccgtgcag cgccaccgtc
     2701 agcagggacg cgaagtagac cttggcgcgg gccgagaact cccgctggtt ctccagggtg
     2761 tggatgaaat tgatgaggaa agacttgctg ttcagcaggt tggagaactg gtagagggcc
     2821 tgctccacca ccggccgccg cggctcaggg atgtccagct tgccggtgat catcacgtcc
     2881 ttgtcgccgt ccttggaggg caggaagaag acgcggtcgg tgtaggtctt gtagtccagc
     2941 acggggatgc cggcctcgtg cacgtcgttg gtctggtcct ccatctcgat catcaggtct
     3001 gtgaattcct tcttgcagcg gtcccgcacg ctctcctcca ggccctccag ctgggacttg
     3061 atcttctcat actctcgttc ggcctgctgg ctcttcctcc agtagcagta gacagacacc
     3121 gcgatgacga ccaccatggg cacgatgacc agcggcaaga tgaggctgag cggcacgtcg
     3181 ctcacccgtg tgtcgtactc cacgcggccc agcacccact cgcgagagcc gaacttcaca
     3241 atgaactcgg gcaggttgtg tgtggtgtct cgtttctgcc gccgcttggg cgggggctgc
     3301 acctccgggg gctcacagta caggtcggtc tccgtcagcg tcttcatggt gcagcgctcg
     3361 gcacccacga aggcctcggc ctcctgcagc gtcatcgcct tgttcagatt ggtgccccgg
     3421 gcgtggatga gcttgttgac ctgcttcttg acgccacctg tgaagttctc aaaggtgggg
     3481 tcaggcacgt actcgaaggc cccggcctct gttctgagca gggcacggtg cccgtccatc
     3541 tcgatcagca ccgtgaggtt gtaggcctct ggctcctcag gcacagccgg ggacaggaag
     3601 acgaccttgg tgtcattgtg gaacacgtag tctgtaccca ccaccgtcat gggctgcagg
     3661 gattcagcct cccgcggcgg ctgccaggac tgcaggggct ccgcgatgac caccatggca
     3721 aacctctgga tcaggctgaa gccctgaccc gtgacgttga tgctgcggcc accactggca
     3781 aagcttcgta gcggctcgaa ggctcgcagt acggggtttt cgcggtaggt gaagaagatg
     3841 ccggggttgg gcacggggga ccccccgtag gagacctcca gaagcatctg gccccgtgtc
     3901 gcctgggggc cagtgacaca ctggagctgc gccccaaact tcgtcacttt acacgggacg
     3961 ccgttgaggg tcacccgcac gtcctcctgg gagcccgtgt ccaggtgggt gccgtggatg
     4021 gtcagtgtgg tgccgcccgc ctgcggtccc tgctgcggct ccacactgag aggcttgggc
     4081 tgttggaagg tgaactggac attgggaggc gaacggccca gtttcccgaa gacgtccacc
     4141 tcgacacccc ccgtgaaagg cgtctccgca gcctcgatca cacacacgat ccgggtggac
     4201 acggagtaac gttccggctg aaaggagcag ttccggccgg ccacagagat cctctggatg
     4261 tcccctgctt ggacgcccaa attggacccc aggatggtga tgcggatgcc cccacccagg
     4321 gggcccgtct caggctggat cctggtgatg acgggcggcg ggcactcgga ggtggtgttg
     4381 cacagggcct catacacgca cctgctctgg cccccgcacc acgcacacct gtagtcgggg
     4441 ttagcggccc ggcacaggct gcagtcgctg cggccaaagg agcagttgta gagggtcaca
     4501 tggagcttgc tgtcgatatt cttgccgtaa gacttgacgt agaggtgcag gggcagcgtc
     4561 tcgttggcat cgtgggacag ctttggggtc cgaaaggcga aggtcccaga ttcctgcatg
     4621 gtcaccggct ccatgaactt gagcaagtca ctgcccacgt gcagggagga acccttcacg
     4681 gtgtccaggt tcttgccctg gaagttcaca tctgtctcgt ggttcatggg gatcaccagg
     4741 gggctgggtc ccaggaactg gggacagctg tcctccatgt gggcacggac gatgccgtcc
     4801 tcagggttgg gcgaagcctc ccggcactcg tggtagcgca ggtcccactg gcaggtccag
     4861 cggttgctca cgcaggagat gcacggcagg ttctcctcca ggctcatggc ctggcggcag
     4921 tcgtagaagg ggtactggta ggacgtgagg aagatgttgc ctcgtctaag gaggagctgg
     4981 atggtcacgg ccacgtggtc ctggcctggc ggtgtgacgg ggatgctgct tggggagttg
     5041 cagatgacgg cctcgccctc cacgcgggcg gggtgtggcg gcgactcccc aaaaaggcac
     5101 agcaactcgt cctcctcgct cagggcaggg agggggctga cggtcagctg cacctccccc
     5161 tgggcccgcc ggctcatgtt ctgtggctgg gcgctggtga cggccacgca ggacttgctt
     5221 cggctccaca gccagtggct ggcctcctcg gcccgcggac actcggcctt ccgggtgcat
     5281 cgtccctcga cgacgcacca gccgcagtag gggtcctggg agtcgcggca ctgggtgcag
     5341 gtcgggtagc tcaggcactc ctgcaccggc agccggaaca ccttgtcctg ggtcatggcg
     5401 tacaggctgc ccaggtctcc agacagtacc aggtcgcgct tgactctctt gtttatctcc
     5461 acaaggatag agtcgtactc tgaggaggtg ccatctgggg tgaggtacac cttgaggatc
     5521 cggccatcag aggtgcccag aaaagcaaca gtgtggttat tttcagcagc cacggtgaca
     5581 gccgtgaggt tcaggcctcc acgctgcagc acggctgtgc ctctgagccc gtcgcggctg
     5641 cccagcgggt agggcaggtg ctccgagcca catgggaagc tcttgctgga gcccggcgcg
     5701 tggccgccgc actggatatc gccgtggaag ggcttgtaga agatgtcacg ggcctcccgg
     5761 gtgcctgtgt aacaggcgtt gcggttggcc tccatcttgg cgtgcacctt gtccagcggg
     5821 aacaggcaga ggcccgcacc gggcccccca ctgctccggc tgtctctgct gaagacagca
     5881 tatagcaccc tgccagagcc aggcgcagcc acggaggcgg ccaggcaggt gccaaaggca
     5941 gcggcgtgga tgtcggggtc ccggcactgc aggtccatct ccaggtagga gtagtagttg
     6001 gggtcttctc tgcacatgcg tgccagcagc gtgcggttcc gggccgggtg cttgtcctgc
     6061 tggttgaaga caaagaagac gtaggggccg tcctcgaagg ccgccacgaa ctgctgtgtg
     6121 ttggtggaca ggtagccggc cttgtaggtg gcgtggtccg tgtaggcttc aaaggcctcc
     6181 ctgctgtcag tccggtccaa cagccgagtg ctcacgatga tgccgttgtc gtgtggccca
     6241 ttgcctttgc ccacaaacag cacgcggtca ccaccaggac ccgtggagct caccagcccc
     6301 actgtggcca cgccctcatc attgctggcc acgaaagact tctccccgct gccgtcctcg
     6361 tagaacaggc ggagggagat gttgctcagg gcgcgcagag cgcagatgcc cttgaagagg
     6421 ctgccgcact ccaccaggcg cttcctggga gggtcgagca gcagcagctg gttgacattg
     6481 tcagtcatct cagcctcatg gcactggctg gcctcgatgg gcggcgtgca cttcttgttg
     6541 tccagggccg ggcccgtggc cacctgctgc tccagctgca gcttcgcatc cagctggtag
     6601 agggcattca ccgcccccag gtacaccacg cctgaggcct catccacagc caggtggttc
     6661 agctctttct cgctgcggaa gaagtccagc ttgcggggcc tcaggctggc acctgcgccc
     6721 agcaggccca gcagggtcag ggcccagagc tgcagtgcca tggtgggcct tgacggccct
     6781 tctgcttttt tgtacaaact tgttgatatc tgcagaattc caccacactg gactagtcga
     6841 cgaaccggtg gatccccctg gggagagagg tcggtgattc ggtcaacgag ggagccgact
     6901 gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag gaacagggcc
     6961 cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct gctctcggcg
     7021 cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc tgccattgct
     7081 ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc
     7141 cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc
     7201 ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat
     7261 gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt
     7321 ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccgt ggaattcgat
     7381 atcaagcttc tcgaggctag tctcgtgatc gataaaattt tgaatttttg taatttgttt
     7441 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
     7501 gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag atccgttcac
     7561 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
     7621 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
     7681 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
     7741 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
     7801 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
     7861 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
     7921 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
     7981 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
     8041 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
     8101 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
     8161 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
     8221 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc
     8281 tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac
     8341 tactttatat ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct
     8401 gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     8461 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     8521 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     8581 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     8641 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     8701 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     8761 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     8821 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     8881 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     8941 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     9001 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     9061 gccagagagc tcccaggctc agatctggtc taaccagaga gacccgttta tgtatcgagc
     9121 taggcactta aatacaatat ctctgcaatg cggcaattca gtggttcgtc caatccatgt
     9181 cagacccgtc tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg
     9241 gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt
     9301 gcaagactac aagagtattg cataagacta cattaagctt gcagctccag cttttgttcc
     9361 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     9421 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9481 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9541 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9601 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9961 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10201 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10561 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10621 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10681 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10741 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10801 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10861 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10921 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10981 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11041 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11101 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11161 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11221 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11281 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11341 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11401 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11461 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11521 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
    11581 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
    11641 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
    11701 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    11761 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
    11821 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
    11881 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
    11941 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    12001 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    12061 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    12121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
    12181 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
    12241 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
    12301 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
    12361 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
    12421 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
    12481 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
    12541 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
    12601 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
    12661 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
    12721 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
    12781 tcttgtcttc gttgggagtg agctagccct tccagtcccc ccttttcttt taaaaagtgg
    12841 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgggcc ctcgacggat
    12901 cccctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
    12961 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
    13021 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
    13081 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
    13141 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
    13201 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
    13261 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
    13321 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
    13381 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
    13441 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
    13501 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
    13561 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
    13621 gttgtgccca gtcatagccg aatagcctct ccacccaagc g
//
