LOCUS       sequence-181212-        8845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Miranda KC, Khromykh T, Christy P, Le TL, Gottardi CJ, Yap AS, Stow 
            JL, Teasdale RD
  TITLE     A dileucine motif targets E-cadherin to the basolateral cell surface
            in Madin-Darby canine kidney and LLC-PK1 epithelial cells.
  JOURNAL   J Biol Chem. 2001 Jun 22. 276(25):22565-72.
  PUBMED    11312273
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Jun 22. 276(25):22565-72."
COMMENT     Sequence Label: E-cadherin-GFP
FEATURES             Location/Qualifiers
     source          1..8845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     284..304
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(313..741)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(423..444)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     635..654
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    787..1011
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1000..1017
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1037..1055
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1037..1054
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1116..1137)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1272..1784)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     1465..1484
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1724..1745
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4571..4590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4572..4590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4635..4838)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4664..4684)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4839..5218
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5390..5409
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5484..5588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5589..5657,5658..6449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5807..5826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6620..7208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7109..7128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7362..7379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7496..7517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7532..7537,7538..7555,7556..7562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7570..7586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7575..7597
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7594..7610
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7594..7610
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7659..7780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7670..7689)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7724..7743
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7954..8748)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8065..8084)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8675..8694
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8815..8845,1..299))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8829..8845,1..119)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa
       61 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
      121 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
      181 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
      241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
      301 ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      361 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      421 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      481 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
      541 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      601 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      661 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      721 atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct
      781 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
      841 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
      901 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
      961 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     1021 atcagcgagc tctagcattt aggtgacact atagaatagg gccctctaga ttacttgatc
     1081 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     1141 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     1201 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1261 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1321 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1381 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1441 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1501 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1561 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1621 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     1681 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     1741 accaggatgg gcaccacccc ggtgaacagc tcctccccct tgctccgcgg gtcgtcctcg
     1801 ccgcctccgt acatgtcagc cagcttcttg aagcgattgc cccattcgtt caagtagtca
     1861 tagtcctggt ctttgtctga ctctgaggag ttcagggagc tcagactagc agcttcggaa
     1921 ccgcttcctt catagtcaaa cacgagcaga gaatcataag gcggggctgt ggggtcagta
     1981 tcagccgctt tcagattttc atcaataaaa tttccaattt catcgggatt ggcagggcgg
     2041 ggaagatacc gggggacact catgagggtt ggtgcaacgt cgttacgagt cacttcaggc
     2101 cgagcgtcca ggcccctgtg cagctggctc aagtcaaagt cctggtcctc ttctccgcct
     2161 ccttcttcat catagtaata aacgttgtcc cgggtgtcat cctctggggg cagtaagggc
     2221 tctttgacca ccgctctcct ccgaagaaac agcaagagca gcagaatcag aattagcaaa
     2281 gcaagaattc ctccaagaat ccccagaatg gcaggaattt gcaatcctgc ttcgacaggc
     2341 tgtgccttcc tacagacgcc ggcggcccct tcacagtcac acacgctgac ctctaaggtg
     2401 gtcacttggt ctttattctg gttatccatg agcttgagat tgattttgta gtcacccacc
     2461 tctaaggcca tctttggctt caaaatgata gattcttggg ttgggtcgtt gtactgaatg
     2521 gtccagttgg cactcgcccc gtgtgttagt tctgctgtga agggagatgt attgggagga
     2581 aggtctgcat caatgatgtt tatgacctga ggctttggat tcctctcaca gaagaatata
     2641 gttcgaggtt ctggtatggg ggcgttgtca ttcacatcag acaggatcag cagaagtgtc
     2701 cctgttccag tagcaactgg agaaccattg tctgtagcta tgattagggc tgtgtacgtg
     2761 ctgttcttca cgtgctcaaa atcctccctg tccagctcag cccgagtgga aatggcacca
     2821 gtgtccggat taatctccag ccagttggca gtgtctctcc aaatccgata tgttattttc
     2881 tgttccataa atgtgtctgg ctcctgggca gtgtaggatg tgatttcctg gcccacgcca
     2941 aagtcctcgg acacttccac tctcttttca ggaggcacaa agatgggggc ttcattcaca
     3001 tccagcacat ccacggtgac ggtggctgtg gaggtggtga gagagacctc aaaaggtacc
     3061 acattcgtca ctgctacgtg tagaatgtac tgctgcttgg cctcaaaatc caagcccttt
     3121 gctgttttca aaatgccatc gttgttcact ggatttgtgg tgacgacaaa ttgtccacca
     3181 tcatcattca atatggtgta tacagcctcc cacgctgggg tattgggggc atcagcatca
     3241 gtcactttca gtgtggtgat tacgacgtta gcctcgttct caggcacctg acccttgtac
     3301 gtggtgggat tgaagatcgg aggattatcg ttggtgtcag tgactgtgat cacagctgtt
     3361 gctgttgtgc ttaacccctc accttgaagg tcagcagctt gaaccaccag ggtatacgta
     3421 gggaaactct ctcggtccag cccagtggtg accacactga tgactcctgt gttcctgtta
     3481 atggtgaaca tatttttgtc agggagctca ggatcttggc tgaggatggt gtaagcgatg
     3541 gcggcattgt aggtgttcac atcatcgtcc gcgtctgtgg ctgtgacctc catcacagag
     3601 gttcctggaa gagcaccttc catgacagac cccttaaaga cctcctgggt gaattcgggc
     3661 ttgttgtcat tctgatcggt taccgtgatc aaaatctcca ttggatcctc aactgcattc
     3721 ccgttggatg acacagcgtg agagaagaga gtgtatgtgg caatgcgttc tctatccaga
     3781 ggctctgtca ccttcagcca tcctgtttct ctttcaataa taaagacacc aacagggggt
     3841 gtgtcagctc cttggccagt gatgctgtag aaaaccttgc cttctttgtc tttgttggat
     3901 ttgatctgaa ccaggttttt aggaaatggg cctttttcat tttctgggca gctgatggga
     3961 ggaataaccc agtctctctt ctgtcttctg aggccaggag aggagttggg aaatgtgagc
     4021 aattctgctt ggattccaga aacggaggcc tgatggggcg gggggcggtg gtggtgcccc
     4081 actgtattca gcgtgacttt ggtggaaaac tttctgtagg tggagtccca ggcgtagacc
     4141 aagaaatgga tctgtgggtt atgaaaccgt agaggccttt tgactgtaat cacaccatct
     4201 gtgcccactt tgaatcgggt gtcgagggaa aaataggctg tcctttgtcg accggtgcaa
     4261 tcttcaaaat tcactctgcc caggacgcgg cctctctcca ggtggcgccg gggcaccgtg
     4321 aacgtgtagc tctcggcgtc aaagccaggg tggcagggct ccggctcctg gcagagccaa
     4381 gaggagacct gcagcagcag cagcagcgcc gagaggctgc ggctccaagg gcccatggct
     4441 ggccggggac gccgagcgag ggcaggcgcc gggtgcggtc gggtcgggcc gggctggagc
     4501 gggctggagt ctgaactgac ttccgcaagc tcacaggtgc tttccggaat tcgatatcaa
     4561 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
     4621 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     4681 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     4741 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     4801 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     4861 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     4921 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     4981 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     5041 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     5101 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     5161 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     5221 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     5281 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     5341 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     5401 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     5461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7381 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7441 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7501 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7561 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7621 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     7681 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7741 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     7801 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     7861 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     7921 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     7981 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     8041 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     8101 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     8161 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     8221 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     8281 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     8341 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     8401 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     8461 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     8521 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     8581 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     8641 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     8701 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     8761 tcatcctgtc tcttgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca
     8821 aaagcctagg cctccaaaaa agcct
//
