LOCUS       sequence-181253-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-81
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    220..793
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(380..399)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     609..626
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(828..1448)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1356..1375
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(1459..1477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1505..1524)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1532..2104
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(2113..2623)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2176..2194)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2672..2789
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2890..2984)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2890..2908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2954..2973)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(3273..3317)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3502..3735
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4228..4353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4400..4580
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4581..4807)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4835..4855)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4835..4853)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4874..4890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4874..4890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4887..4909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4898..4914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4922..4928,4929..4946,4947..4952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4967..4988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5105..5122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5276..5864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5356..5375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6035..6826,6827..6895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6658..6677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6896..7000)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7026..7481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7163..7184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7375..7394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7608..7630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7622..7639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7623..7639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7649..7667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7688..7823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7863..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7874..7893)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7928..7947
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8056..8289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(8417..8816,1..200))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8500..8520)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
       61 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
      121 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
      181 gtgggcttgt actcggtcat ggtaagctcc ggtggatcct tatcatcgtg tttttcaaag
      241 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
      301 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
      361 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
      421 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
      481 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
      541 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
      601 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
      661 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
      721 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
      781 ggagggagag gggcagatcc ctagaactcc cgggagccgc tgtacgcgga cccactttca
      841 catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc
      901 tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca
      961 gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa
     1021 cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct
     1081 tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt
     1141 gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta
     1201 gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg
     1261 tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc
     1321 caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct
     1381 tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat
     1441 ctagacatat caattcgccc tatagtgagt cgtattacaa ttctttgcca aaatgatgag
     1501 acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat
     1561 ggttagcaga ggggcccggt ttggactcag agtattttat cctcatctca aacagtgtat
     1621 atcattgtaa ccataaagag aaaggcagga tgatgaccag gatgtagttg tttctaccaa
     1681 taagaatatt tccacgccag ccagaattta tatgcagaaa tattctacct tatcatttaa
     1741 ttataacaat tgttctctaa aactgtgctg aagtacaata taatataccc tgattgcctt
     1801 gaaaaaaaag tgattagaga aagtacttac aatctgacaa ataaacaaaa gtgaatttaa
     1861 aaattcgtta caaatgcaag ctaaagttta acgaaaaagt tacagaaaat gaaaagaaaa
     1921 taagaggaga caatggttgt caacagagta gaaagtgaaa gaaacaaaat tatcatgagg
     1981 gtccatggtg atacaaggga catcttccca ttctaaacaa caccctgaaa actttgcccc
     2041 ctccatataa catgaatttt acaatagcga aaaagaaaga acaatcaagg gtccccaaac
     2101 tcacagatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     2161 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     2221 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     2281 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     2341 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     2401 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     2461 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     2521 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     2581 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccctcgagc cgcggccaaa
     2641 gtggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     2701 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     2761 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctcgagg
     2821 tcgagaattc aaaaaaggac cactttccct actttactcg agtaaagtag ggaaagtggt
     2881 cctccggtgt ctctatcact gatagggaac ttataagtct ctatcactga tagggatttc
     2941 acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcggc cgctcgaggc
     3001 tagtctcgtg atcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
     3061 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     3121 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     3181 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     3241 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     3301 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     3361 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     3421 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     3481 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     3541 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     3601 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     3661 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     3721 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     3781 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     3841 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     3901 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     3961 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     4021 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     4081 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     4141 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     4201 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     4261 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     4321 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     4381 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     4441 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     4501 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     4561 tggtctaacc agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg
     4621 caatgcggca attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat
     4681 aaggcacgat cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct
     4741 tgtaaggcat gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa
     4801 gactacatta agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     4861 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7021 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7081 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7141 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7201 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7261 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7321 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7381 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7441 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     7501 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7561 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7621 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     7681 gtccctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     7741 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     7801 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat
     7861 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7921 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7981 agttcctctc actctctgat attcatttct ttgcaagtta taaatactga ataataagat
     8041 gacatgaact actactgcta gagattttcc acactgacta aaagggtctg agggatctct
     8101 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     8161 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     8221 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     8281 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     8341 tcattggtct taaaggtacc gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg
     8401 ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     8461 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     8521 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     8581 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     8641 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     8701 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     8761 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtcc
//
