LOCUS       sequence-181255-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-#1
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..35
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(237..857)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     765..784
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(868..886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(914..933)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          941..1513
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1522..2032)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1585..1603)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2081..2198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2298..2392)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2326..2344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2362..2381)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(2681..2725)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2910..3143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3636..3761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3808..3988
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3989..4215)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4243..4263)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4243..4261)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4282..4298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4282..4298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4295..4317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4306..4322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4330..4336,4337..4354,4355..4360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4375..4396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4513..4530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4684..5272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4764..4783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5443..6234,6235..6303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6066..6085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6304..6408)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6434..6889)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6571..6592)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6783..6802
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7016..7038
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7030..7047
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7031..7047
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7057..7075
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7096..7231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7271..7392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7282..7301)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7336..7355
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7464..7697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(7825..8424)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7908..7928)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8405..8424
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(8444..8815,1..202)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8604..8623)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
       61 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
      121 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
      181 aggggggggg gagggagagg ggcagatccc tagaactccc gggagccgct gtacgcggac
      241 ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag
      301 aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc
      361 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc
      421 aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt
      481 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
      541 gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta
      601 aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
      661 tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt
      721 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
      781 gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt
      841 ttatctaatc tagacatatc aattcgccct atagtgagtc gtattacaat tctttgccaa
      901 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      961 catgaacatg gttagcagag gggcccggtt tggactcaga gtattttatc ctcatctcaa
     1021 acagtgtata tcattgtaac cataaagaga aaggcaggat gatgaccagg atgtagttgt
     1081 ttctaccaat aagaatattt ccacgccagc cagaatttat atgcagaaat attctacctt
     1141 atcatttaat tataacaatt gttctctaaa actgtgctga agtacaatat aatataccct
     1201 gattgccttg aaaaaaaagt gattagagaa agtacttaca atctgacaaa taaacaaaag
     1261 tgaatttaaa aattcgttac aaatgcaagc taaagtttaa cgaaaaagtt acagaaaatg
     1321 aaaagaaaat aagaggagac aatggttgtc aacagagtag aaagtgaaag aaacaaaatt
     1381 atcatgaggg tccatggtga tacaagggac atcttcccat tctaaacaac accctgaaaa
     1441 ctttgccccc tccatataac atgaatttta caatagcgaa aaagaaagaa caatcaaggg
     1501 tccccaaact cacagatccc cctggggaga gaggtcggtg attcggtcaa cgagggagcc
     1561 gactgccgac gtgcgctccg gaggcttgca gaatgcggaa caccgcgcgg gcaggaacag
     1621 ggcccacact accgccccac accccgcctc ccgcaccgcc ccttcccggc cgctgctctc
     1681 ggcgcgccct gctgagcagc cgctattggc cacagcccat cgcggtcggc gcgctgccat
     1741 tgctccctgg cgctgtccgt ctgcgagggt actagtgaga cgtgcggctt ccgtttgtca
     1801 cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt aggggcggag caggaagcgt
     1861 cgccgggggg cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa cggacgtgaa
     1921 gaatgtgcga gacccagggt cggcgccgct gcgtttcccg gaaccacgcc cagagcagcc
     1981 gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aaccccaacc ccctcgagcc
     2041 gcggccaaag tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     2101 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     2161 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     2221 gtctcgaggt cgagaattca aaaagccgga acatgaccat caatctcttg aattgatggt
     2281 catgttccgg cccggtgtct ctatcactga tagggaactt ataagtctct atcactgata
     2341 gggatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcggccg
     2401 ctcgaggcta gtctcgtgat cgataccgtc gagatccgtt cactaatcga atggatctgt
     2461 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     2521 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     2581 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     2641 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     2701 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     2761 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     2821 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     2881 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     2941 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     3001 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     3061 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     3121 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     3181 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     3241 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     3301 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     3361 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     3421 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     3481 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     3541 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     3601 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     3661 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     3721 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     3781 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     3841 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     3901 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     3961 ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac ttaaatacaa
     4021 tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc
     4081 ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt
     4141 tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta
     4201 ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt gagggttaat
     4261 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4321 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4381 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4441 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4501 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4561 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4621 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5101 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6421 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     6481 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6541 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6601 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6661 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6721 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6781 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6841 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     6901 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     6961 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     7021 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     7081 ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     7141 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     7201 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg atagctagag
     7261 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7321 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7381 aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     7441 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     7501 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     7561 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     7621 gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga
     7681 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     7741 cttgtaagtc attggtctta aaggtaccga tgcatggggt cgtgcgctcc tttcggtcgg
     7801 gcgctgcggg tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg
     7861 cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg
     7921 gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc
     7981 cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc
     8041 gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat
     8101 ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc
     8161 gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc
     8221 gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag
     8281 ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc
     8341 ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg
     8401 caccgtgggc ttgtactcgg tcatggtaag ctccggtgga tccttatcat cgtgtttttc
     8461 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa
     8521 cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct
     8581 gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa
     8641 ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac
     8701 gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg
     8761 tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcac
//
