LOCUS       sequence-181256-         163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 163)
  AUTHORS   Salvail-Lacoste A, Di Tomasso G, Piette BL, Legault P
  TITLE     Affinity purification of T7 RNA transcripts with homogeneous ends 
            using ARiBo and CRISPR tags.
  JOURNAL   RNA. 2013 Jul;19(7):1003-14. doi: 10.1261/rna.037432.112. Epub 2013 
            May 8.
  PUBMED    23657939
REFERENCE   2  (bases 1 to 163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037432.112"; journalName: "RNA"; date: "2013-07"; 
            volume: "19"; issue: "7"; pages: "1003-14"
COMMENT     Sequence Label: pARiBo4
FEATURES             Location/Qualifiers
     source          1..163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..44
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 taatacgact cactatagcg ccgaactggg ccctgaagaa gggctcagtt gacgaggtag
       61 acgttatcga gatttcggcg gatgcgtctc ggttgttcat cacaaccgca aacttttact
      121 taaatcatta aggtgactta gtggacaaag gtgaaagtgt gat
//
