LOCUS       sequence-18127-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  TITLE     Cross lab plasmids
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS408
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..55)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(39..55)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(92..112)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(92..110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(131..147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(144..166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    155..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(179..185,186..203,204..209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    224..245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(362..379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(613..632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtatcgatag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc
       61 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
      121 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      181 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      241 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcg
//
