LOCUS       sequence-181270-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR guide RNA targeting human RRBP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: lentiGuide-RRBP1-1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..75)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(226..246)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(424..468)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    653..886
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1379..1504
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1551..1731
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1732..1958)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1986..2006)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1986..2004)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2025..2041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2025..2041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2038..2060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2049..2065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2073..2079,2080..2097,2098..2103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2118..2139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2256..2273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2427..3015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2507..2526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3186..3977,3978..4046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3809..3828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4047..4151)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4177..4632)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4314..4335)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4526..4545
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4759..4781
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4773..4790
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4774..4790
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4800..4819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4800..4818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4839..4974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5014..5135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5025..5044)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5079..5098
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5213..5446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5518..6106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5624..5635
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6033..6053
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6122..6721)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6205..6225)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6702..6721
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6734..7912)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6743..7681
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6766..6786)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8051..8168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(8224..8299)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(join(8321..8323,1..246))
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
       61 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      121 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
      181 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
      241 gccctctctg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
      301 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
      361 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
      421 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
      481 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
      541 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
      601 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
      661 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
      721 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
      781 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
      841 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
      901 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
      961 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
     1021 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
     1081 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga
     1141 gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt
     1201 gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg
     1261 cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc
     1321 cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga
     1381 cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca
     1441 ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag
     1501 agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat
     1561 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     1621 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     1681 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta
     1741 tcgagctagg cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat
     1801 ccatgtcaga cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac
     1861 caatcggcat gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac
     1921 catgttgcaa gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt
     1981 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     2041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4141 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4201 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     4261 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     4321 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     4381 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     4441 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     4501 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     4561 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4621 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     4681 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     4741 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     4801 aatacgactc actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca
     4861 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     4921 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     4981 agttaggggc gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa
     5041 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     5101 tatttgtaac cattataagc tgcaataaac aagttcctct cactctctga tattcatttc
     5161 tttgcaagtt ataaatactg aataataaga tgacatgaac tactatacgt actgctagag
     5221 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     5281 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     5341 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     5401 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     5461 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg
     5521 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     5581 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     5641 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa
     5701 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     5761 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     5821 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     5881 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     5941 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     6001 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     6061 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg acttaacgcg
     6121 ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
     6181 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
     6241 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
     6301 tgcccagacc cttgccctgg tggtcgggcg agactccgac ggtggccagg aaccacgcgg
     6361 gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg
     6421 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
     6481 tctccggcgt ggtccagacc gccacggcgg cgccgtcgtc cgcgacccac accttgccga
     6541 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
     6601 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
     6661 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
     6721 tggtggccgt acgtcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
     6781 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
     6841 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
     6901 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
     6961 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg
     7021 cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt
     7081 ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg
     7141 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca
     7201 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc
     7261 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa
     7321 aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa
     7381 aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga
     7441 gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct
     7501 caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac
     7561 ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag
     7621 gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta
     7681 cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
     7741 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
     7801 agtttacccc gcgccacctt ctctaggcac cggatcaatt gccgacccct ccccccaact
     7861 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag ccaattccca
     7921 ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta
     7981 tccatctttg cacccgggcc ccctcgagcc ggcgccaaag tggatctctg ctgtccctgt
     8041 aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
     8101 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
     8161 ttttaaaatt gtggatgaat actgccattt gtctcaagat ctagttacgc caagcttaaa
     8221 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg
     8281 ctatttctag ctctaaaacc ccattatcgt tgctcctgtc ggt
//
