LOCUS       sequence-181272-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR guide RNA targeting human SYNJ2BP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: lentiGuide-SYNJ2BP-1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     369..386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    503..524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(539..544,545..562,563..569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    577..593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     582..604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     601..617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     601..617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     636..656
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        638..656
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        684..910
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             911..1091
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1138..1263
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1756..1989
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2174..2218
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2396..2644
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2396..2416
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2567..2586
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2666..2741
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2797..2914
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3053..4231
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3284..4222
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4179..4199
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4244..4843
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4244..4263)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4740..4760
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4859..5447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4912..4932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5330..5341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5519..5752
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5830..5951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5867..5886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5921..5940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5991..6126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6146..6165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6147..6165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6175..6192)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6175..6191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6184..6206)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6333..6788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6420..6439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6630..6651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6814..6918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6919..6987,6988..7779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7137..7156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7950..8323,1..215)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
       61 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      241 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      301 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      361 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      421 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      601 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
      661 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      721 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      781 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      841 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      901 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      961 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     1021 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1081 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1141 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1201 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1261 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1321 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1381 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1441 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1501 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1561 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1621 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1681 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1741 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1801 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1861 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1921 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1981 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     2041 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2101 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2161 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2221 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2281 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2341 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg
     2401 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     2461 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     2521 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     2581 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     2641 caccgaagag atcaatctta ccagagtttt agagctagaa atagcaagtt aaaataaggc
     2701 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa
     2761 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2821 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2881 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2941 tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga
     3001 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     3061 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     3121 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     3181 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     3241 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     3301 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     3361 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     3421 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     3481 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3541 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3601 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3661 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3721 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3781 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3841 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3901 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3961 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     4021 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     4081 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     4141 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     4201 aagttttttt cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc
     4261 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4321 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4381 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4441 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4501 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4561 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4621 caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4681 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4741 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4801 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa
     4861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     5461 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5521 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     5761 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5821 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5941 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6001 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6061 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6121 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6181 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6241 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6301 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6361 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6421 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6481 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6541 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6601 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6661 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6721 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6781 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7621 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tga
//
