LOCUS       sequence-181278-        8734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged human SYNJ2BP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: pLX_TRC208-SYNJ2BP-V5
FEATURES             Location/Qualifiers
     source          1..8734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(147..735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(227..246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(906..1697,1698..1766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1529..1548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1767..1871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1897..2352)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2034..2055)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2246..2265
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2479..2501
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2493..2510
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2494..2510
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2520..2539
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2520..2538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2559..2694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2734..2855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2745..2764)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2799..2818
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2933..3166
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3238..3826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3344..3355
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3753..3773
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3845..3886)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3888..3912
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4374..4577)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4403..4423)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4578..4881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4998..5115
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5172..6191)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     promoter        complement(6203..6703)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6266..6284)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(6878..6922)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7107..7340
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7833..7958
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8005..8185
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8186..8412)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8440..8460)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8440..8458)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8479..8495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8479..8495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8492..8514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8503..8519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8527..8533,8534..8551,8552..8557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8572..8593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8710..8727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
       61 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1861 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     1921 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1981 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2041 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2101 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2161 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2221 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2281 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2341 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2401 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2461 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     2521 aatacgactc actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca
     2581 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     2641 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     2701 agttaggggc gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa
     2761 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2821 tatttgtaac cattataagc tgcaataaac aagttcctct cactctctga tattcatttc
     2881 tttgcaagtt ataaatactg aataataaga tgacatgaac tactatacgt actgctagag
     2941 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3001 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3061 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     3121 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     3181 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg
     3241 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     3301 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     3361 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa
     3421 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     3481 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     3541 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     3601 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     3661 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     3721 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     3781 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg acttaacgcg
     3841 ttcacgtaga atcgagaccg aggagagggt tagggatagg cttaccaacc actttgtaca
     3901 agaaagttgg gcaaagttgt tgccggtatc tcatgaaagc ccaggctgct accatggtga
     3961 gggcaaacac tggcaccagc accataaata tgggaatacc acttgggtcc ccttcacctc
     4021 gatgtcctat aggtccattc tgcacctgta acctgtgctg cactctcaga gacacagcat
     4081 agcctgcatt acgaaagagg tctacagcat cctggtgcag caggttcttt aggtcttggc
     4141 catttaccga aaggatctta tcaccctcct ggagccgccc atccagggcc gcagccccat
     4201 tttctttgat gcggctgacg tagatgccac tgtcgttgga gacatactgc tgatctgtcc
     4261 caccgacgat gttgaagccc agccctgagg ggccgcgcgt caggttaatt tcctcctcag
     4321 tgaccaaata atcgacgcgg ccattcatgg tggatcccga cagttagcca gagagctctg
     4381 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     4441 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     4501 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     4561 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     4621 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     4681 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     4741 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     4801 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     4861 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     4921 attgattact attaataacc ccgggccccc tcgagccggc gccaaagtgg atctctgctg
     4981 tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt
     5041 tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc
     5101 ccttttcttt taaaattgtg gatgaatact gccatttgtc tcaagatcta gaattctcat
     5161 tactgcagat cctttgccct aggacgagtg ctggggcgtc tgtttccact atcggcgagc
     5221 acttccacac agccatcggt ccagacggca gcgcttctgc gggcgatttg tgtacgcccg
     5281 acagtcccgg ctccggaccg gacgattgcg tcgcagcgac cctgagccca agctgcatca
     5341 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatataa
     5401 gcccggagcc gaggagatcc tgcaagctcg ggatgcctcc gctcgaagta gcgggtctgc
     5461 tgctccatac aagccaacca gggcctccag aagaagatgt tggcgacctc gtattgggaa
     5521 tccccgaaca tggcctcgct ccagtcaatg acggctgtta tgcggccatt gtcggtcagg
     5581 acattgttgg agccgaaatc ggcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     5641 caaagcatca gctcatcgag agcctgggcg acggaggcac tgacggtgtc gtccatcaca
     5701 gtttgccagt gatacacatg gggatcagca atagcgcaga tgaaatcgcg ccatgtagtg
     5761 tattgaccga ttccttgagg tccgaatggg ccgaacccgc tggtctggct aagatcggcg
     5821 gcagcgatgg catccatggc ctcggcgacg ggctggagaa cagcgggcag ttcggtttca
     5881 ggcaggtctt gcaaggtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     5941 ctgaactccc caatgtcaag cacttcggga atagggagag cggcggatgc aaagtgccga
     6001 taaacatagc gatctttgta gaaaccatcg gcgcagctat tcacccgcag gacatatcca
     6061 cgccctccca catcgaagct gaaagcccga gattcttcgc cctcggagag ctgcatcagg
     6121 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagaggtagc ggtgagttca
     6181 ggctttttca tggtggtacc ccctggggag agaggtcggt gattcggtca acgagggagc
     6241 cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca
     6301 gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct
     6361 cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca
     6421 ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc
     6481 acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg
     6541 tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga
     6601 agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc
     6661 cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caagggtccc ctcggggttg
     6721 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     6781 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     6841 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     6901 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     6961 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     7021 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     7081 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     7141 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     7201 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     7261 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     7321 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     7381 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     7441 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccgcttgc
     7501 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     7561 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     7621 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     7681 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     7741 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     7801 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     7861 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     7921 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     7981 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     8041 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8101 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8161 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
     8221 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     8281 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     8341 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     8401 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     8461 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8701 ttccgcttcc tcgctcactg actcgctgcg ctcg
//
