LOCUS       sequence-181284-        3406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of dusp5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Edusp5E1
FEATURES             Location/Qualifiers
     source          1..3406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(259..286)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(378..464)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     513..535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     527..544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     528..544
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    584..679
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1442..1566
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1638..1657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1639..1657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1662..1678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1662..1678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1780..1799)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1791..2600
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1858..1877)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2747..3335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3236..3255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
       61 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      241 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      301 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
      361 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      421 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
      481 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
      541 cagtcttaag ctcgggcccg agttaacgct accatggagc tccaaataat gattttattt
      601 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt tataatgcca
      661 actttgtata gaaaagttgg ttaaaacaac tacactttca taaaggtcaa catctacata
      721 tctacaagca ttacattttt tgttttcaac tgctgtataa gcaacatgtg ctaacatgta
      781 ataggcggca agacagctga gtaaaatctg caaaacacac acacacacac acactcatgc
      841 acttgagcag cagatgaaga tctgtctgtg gttcattagt aatgaggctc tcgcagacac
      901 acctcatctc caccctgctg ctgtggaact gggagtttta cgccacacca cagtttcatt
      961 ctcactctaa atggaaactg tgcgtgcact ccccaattta agcacacaac ctaatttttc
     1021 aaaagatggc cacaaagttt gagctgacca gttggatgtt gtgttgtttt tgctgagtcc
     1081 tcgaggtatg ggatgtttac atgccctgag gatgtgcatt gtttctcatg cctggtcttg
     1141 tagctgccag tctagatctg ctgagagctg ttcccatttc ctgctttaca ggaagtgtcg
     1201 gcccccactg gtctttgtca ctcagagaga caggaagagg aaacggccca caggaaacat
     1261 ctcccccact tgctacgaat catcccgcaa catgagccta caagcacaaa ctgttttcat
     1321 ttagttagtt tatggtagaa cacactgtga aagtgaagat gtttgcaatg gtctgaccta
     1381 aatgctttta tttgtgtgac acaatgtaat taggacagtt aatgcatgat taagtagtcg
     1441 aacaagtttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
     1501 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc
     1561 actatgaacc aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc
     1621 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1681 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1741 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1801 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     1861 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     1921 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     1981 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     2041 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     2101 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     2161 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     2221 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     2281 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     2341 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     2401 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     2461 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     2521 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     2581 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     2641 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     2701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2881 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3361 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgc
//
