LOCUS       sequence-181289-        5347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV construct encoding GFP control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Han KS, Cooke SF, Xu W
  TITLE     Experience-Dependent Equilibration of AMPAR-Mediated Synaptic 
            Transmission during the Critical Period.
  JOURNAL   Cell Rep. 2017 Jan 24;18(4):892-904. doi: 
            10.1016/j.celrep.2016.12.084.
  PUBMED    28122240
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.12"; journalName: "Cell Rep"; date: 
            "2017-01-24- 24"; volume: "18"; issue: "4"; pages: "892-904"
COMMENT     Sequence Label: GFP pA_EB_CAGW
FEATURES             Location/Qualifiers
     source          1..5347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   439..568
                     /label=AAV2 ITR
     rep_origin      640..1095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(727..746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     937..958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1121..1225
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1226..1294,1295..2086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1444..1463)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2257..2845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2746..2765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3082..3211
                     /label=AAV2 ITR
     polyA_signal    3373..3494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3410..3429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3464..3483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    3537..4125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3643..3654
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4052..4072
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(4163..4182)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4165..4297
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(join(4323..5036,5037..5039,5040..5042))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4326..5036,5037..5039,5040..5042))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4368..4389)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4717..4736
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4976..4997
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5039..5048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5100..5119)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5181..5203)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa aattgggggt
       61 ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc gaccatggta
      121 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      181 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      241 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
      301 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
      361 ggtacttcga agtgaattcg atcttcctag agcatggcta cgtagataag tagcatggcg
      421 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
      481 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
      541 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc
      601 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      961 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1021 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1081 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1141 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1201 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1261 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1321 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1381 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1441 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1501 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1561 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1621 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1681 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1741 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1801 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1861 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1921 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1981 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2041 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2101 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2161 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2401 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2881 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2941 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3001 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3061 ttggccgatt cattaatgca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
     3121 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
     3181 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
     3241 ttatctacgt agccatgctc taggaagatc cttatcgatt ttaccacatt tgtagaggtt
     3301 ttacttgctt taaaaaacct cccacatctc cccctgaacc tgaaacataa aatgaatgca
     3361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3481 atcaatgtat cttatcatgt ctgctcgaag cggccgcccg ggtcgacggt atcgatgcgg
     3541 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
     3601 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
     3661 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa
     3721 ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
     3781 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
     3841 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
     3901 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
     3961 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
     4021 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
     4081 aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat
     4141 ctagtcgcga gctccactcg acacctgtgg agagaaaggc aaagtggatg tcagtaagac
     4201 caataggtgc ctatcagaaa cgcaagagtc ttctctgtct cgacaagccc agtttctatt
     4261 ggtctcctta aacctgtctt gtaaccttga tacttacctg cggcctcgag tccggggccg
     4321 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     4381 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     4441 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     4501 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     4561 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     4621 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     4681 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     4741 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     4801 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     4861 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     4921 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     4981 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     5041 atggtggcga ccggtacccg gggatcccga tccaattctt tgccaaaatg atgagacagc
     5101 acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta
     5161 gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc
     5221 ccccgaaggc agccgtcccc ccgcggacag ccccgagccg ctccccccgc acaaagggcc
     5281 ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac
     5341 caagcgc
//
