LOCUS       sequence-181293-        5853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector has been discontinued as of 1/16/2019. See Comments 
            below for information on the updated version of the pSGDluc vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Loughran G, Howard MT, Firth AE, Atkins JF
  TITLE     Avoidance of reporter assay distortions from fused dual reporters.
  JOURNAL   RNA. 2017 Apr 25. pii: rna.061051.117. doi: 10.1261/rna.061051.117.
  PUBMED    28442579
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Apr 25. pii: rna.061051.117. doi: 10.1261/rna.061051.117."
COMMENT     Sequence Label: pSGDluc
FEATURES             Location/Qualifiers
     source          1..5853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(217..1008,1009..1077))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    1182..1230
                     /note="synthetic polyadenylation signal"
     misc_feature    1244..1335
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1284..1303
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1441..1798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1441..1632
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1464..1484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1649..1784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1805..1824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1805..1823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1853..1862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1859..2791
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             2843..2908
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2965..3030
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3049..4698
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4742..4863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4753..4772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4807..4826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5111..5128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5282..5853,1..17))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5362..5381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
       61 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      181 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
      241 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
      301 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
      361 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
      421 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
      481 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
      541 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
      601 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
      661 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
      721 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
      781 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
      841 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
      901 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
      961 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     1021 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     1081 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     1141 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     1201 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     1261 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     1321 ccagaacatt tctctggcct aactggccgg tacctgagct cgctagcttc gaggatatca
     1381 gatcagcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
     1441 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1801 gctctaatac gactcactat agggagcttg gcaatccggt actgttggta aagccaccat
     1861 ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg
     1921 ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa
     1981 gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag
     2041 gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat
     2101 gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct
     2161 caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg
     2221 gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt
     2281 ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga
     2341 ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt
     2401 cgtcgagacc atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc
     2461 ctacctggag ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg
     2521 cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa
     2581 cgcctacctt cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt
     2641 cttttccaac gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt
     2701 gaagggcctc cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag
     2761 cttcgtggag cgcgtgctga agaacgagca gtatttctac acctcgagag aagccagaca
     2821 caaacagaaa attgtggcac cggtgaaaca gactttgaat tttgaccttc ttaagctggc
     2881 gggagacgtc gagtccaacc ccgggcccta ctcgagcaga agcttagatc tgaggcacgg
     2941 cataagcaaa agatcgtagc cccagtaaag caaacactca acttcgatct actcaaactc
     3001 gcaggtgatg tggaatctaa tccaggacct ttcggatctg ccaccattga agatgccaaa
     3061 aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag
     3121 ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca
     3181 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa
     3241 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc
     3301 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct
     3361 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
     3421 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc
     3481 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac
     3541 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc
     3601 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc
     3661 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc
     3721 atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac
     3781 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg
     3841 taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc
     3901 ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac
     3961 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag
     4021 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca
     4081 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg
     4141 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac
     4201 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc
     4261 gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac
     4321 tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag
     4381 ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc
     4441 gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc
     4501 gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag
     4561 gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga
     4621 ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc
     4681 ggcaagatcg ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg
     4741 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4801 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4861 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     4921 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
     4981 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     5041 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     5101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5821 gtttttttgt ttgcaagcag cagattacgc gca
//
