LOCUS       sequence-181297-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide 
            repeats) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: pcDNA4 HisMax-V5-GFP-RRBP1
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             961..1002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             join(1012..1014,1015..1017,1018..1728)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1057..1078)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1318..1337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1665..1686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6419..6436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6425..6649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6695..7123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6782..6801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6992..7013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7132..7152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7137..7466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7317..7452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7514..7561
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7580..7954
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8084..8205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8121..8140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8175..8194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8254..8270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8254..8270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8267..8289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8278..8294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8302..8308,8309..8326,8327..8332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8347..8368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8485..8502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8656..9244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8736..8755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9415..10206,10207..10275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10038..10057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10276..10380)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgtcgc ccgggatcct
      961 ggcaagccga tccctaaccc tctgctgggc ctggacagca ctacggatcc aatggtgagc
     1021 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1081 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1141 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1201 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1261 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1321 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1381 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1441 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1501 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1561 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1621 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1681 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggccggact
     1741 cagatccgaa agcaagccag gatggatatt tacgacactc aaaccttggg ggttgtggtc
     1801 tttggaggat tcatggttgt ttctgccatt ggcatcttcc tggtgtcgac tttctccatg
     1861 aaggaaacgt catatgaaga agccctagcc aaccagcgca aggagatggc gaaaactcac
     1921 caccagaaag tcgagaagaa aaagaaggag aaaacagtgg agaagaaagg aaagaccaag
     1981 aaaaaggaag agaaacctaa tgggaagata cctgatcatg atccagcccc caatgtgact
     2041 gtcctccttc gagaaccagt gcgggctcct gctgtggctg tggctccaac cccagtgcag
     2101 ccccccatta tcgttgctcc tgtcgccaca gttccggcca tgccccagga gaagctggcc
     2161 tcctccccca aggacaaaaa gaagaaggag aaaaaagtgg caaaagtgga accagctgtc
     2221 agctctgtag tgaattccat ccaggttctc acttcgaagg ctgccatctt ggaaactgct
     2281 cccaaggagg tgccgatggt ggtggtgccc ccagtgggtg ccaagggcaa cacaccagcc
     2341 actggcacta ctcagggcaa aaaggcggag gggactcaga atcaaagcaa aaaggctgaa
     2401 ggagccccaa accagggcag aaaggcagag ggaaccccaa accagggcaa aaagacagag
     2461 ggaaccccaa accaagggaa aaaggcagag ggaaccccaa accaaggcaa aaaggcagaa
     2521 ggaaccccaa accaaggcaa aaaggcggag ggggcccaga accagggtaa aaaggtagat
     2581 acaaccccaa accaggggaa aaaggtggag ggggccccaa cccagggcag aaaggccgag
     2641 ggggctcaga accaggccaa aaaggtagaa ggggcccaga accagggcaa aaaggcagag
     2701 ggggcccaga atcagggcaa aaagggagag ggggcccaga accagggcag gaaggccgag
     2761 ggggcccaga accagggcag gaaggccgag ggggcccaga atcagggcaa gaaggccgag
     2821 ggggcccaga atcagggcaa gaaggccgag ggggcccaga atcagggcaa gaaggctgag
     2881 ggggctcaga accagggcaa aaaggccgag ggggctcaga accagggcaa aaaagtagaa
     2941 ggggcccaga accagggcaa gaaggctgag ggtgcccaga accagggcaa aaaggccgag
     3001 ggggcccaga atcagggcaa aaaggccgag ggggcccaga accagggcaa gaaggcagag
     3061 ggggcccaga accagggcaa gaaggccgag ggggcccaga accaggacaa gaaggccgag
     3121 ggggcccaga accagggcag gaaggccgag ggggcccaga accagggcag gaaggccgag
     3181 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
     3241 ggggccccga accagggcaa gaaggccgag gggaccccga accagggcaa gaaggccgag
     3301 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
     3361 gggaccccga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
     3421 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
     3481 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
     3541 ggtgctcaga accagggcaa aaaagtagaa ggggcccaga accagggcaa gaaggccgag
     3601 ggggcccaga accagggcaa gaaggccgag ggggctcaga accagggcaa aaaggccgag
     3661 ggagcccaga accagggcca aaaaggagag ggagcccaga atcagggtaa aaagacagaa
     3721 ggggctcagg gcaaaaaagt agaaggggcc cagaaccagg gcaagaaggc cgagggggcc
     3781 cagaaccagg gcaaaaaggc agattcggtt gctaatcagg gcacaaaggt agagggtatt
     3841 acaaaccagg ggaaaaaagc agaagggtcc cccagtgaag gcaaaaaggc agaagggtcc
     3901 cccaaccaag gcaaaaaggc agacgcagct gccaatcagg gtaaaaagac agagtcagct
     3961 tctgtccagg gcagaaatac agatgtggcc cagagcccag aggcaccaaa gcaagaggct
     4021 cctgccaaga agaagtctgg ttcaaagaaa aaaggtgagc ctgggccccc agatgccgac
     4081 ggccctctct acctccccta caagacgctg gtctccacgg ttgggagcat ggtgttcaac
     4141 gagggcgagg cccagcggct catcgagatc ctgtctgaga aggctggcat cattcaggac
     4201 acctggcaca aggccactca gaagggtgac cctgtggcga ttctgaaacg ccagctggaa
     4261 gagaaggaaa aactgctggc cacagaacag gaagatgcgg ctgtcgccaa gagcaaactg
     4321 agggagctca acaaggagat ggcagcagaa aaggccaaag cagcagccgg ggaggccaaa
     4381 gtgaaaaagc agctggtggc ccgggagcag gagatcacgg ctgtgcaggc acgcatgcag
     4441 gccagctacc gggagcacgt gaaggaggtg cagcagctgc agggcaagat ccggactctt
     4501 caggagcagc tggagaatgg ccccaacacg cagctggccc gcctgcagca ggagaactcc
     4561 atcctgcggg atgccttgaa ccaggccacg agccaggtgg agagcaagca gagcgcagag
     4621 ctggccaagc ttcggcagga gctcagcaag gtcagcaaag agctggtgga gaagtcagag
     4681 gctgtgcggc aagatgagca gcagcggaaa gctctggaag ccaaggcagc tgccttcgag
     4741 aagcaggtcc tgcagctgca ggcgtcccac agggagagtg aggaggccct gcagaagcgc
     4801 ctggacgagg tcagccggga gctgtgccac acgcagagca gccacgccag cctccgggcg
     4861 gatgccgaga aggcccagga gcaacagcag cagatggccg agctgcacag caagttacag
     4921 tcctccgagg cggaggtgcg cagcaaatgc gaggagctga gtggcctcca cgggcagctc
     4981 caggaggcca gggcggagaa ctcccagctc acagagagaa tccgttccat tgaggccctg
     5041 ctggaggcgg gccaggcgcg ggatgcccag gacgtccagg ccagccaggc ggaggctgac
     5101 cagcagcaga ctcgcctcaa ggagctggag tcccaggtgt cgggtctgga gaaggaggcc
     5161 atcgagctca gggaggccgt cgagcagcag aaagtgaaga acaatgacct ccgggagaag
     5221 aactggaagg ccatggaggc actggccacg gccgagcagg cctgcaagga gaagctgcac
     5281 tccctgaccc aggccaagga ggaatcggag aagcagctct gtctgattga ggcgcagacc
     5341 atggaggccc tgctggctct gctcccagaa ctctctgtct tggcacaaca gaattacacc
     5401 gagtggctgc aggatctcaa agagaaaggc cccacgctgc tgaagcaccc gccagctccc
     5461 gcggagccct cctcggacct ggcctccaag ttgagggagg ccgaggagac gcagagcaca
     5521 ctgcaggccg agtgtgacca gtaccgcagc atcctggcgg agacggaggg catgctcaga
     5581 gacctgcaga agagcgtgga ggaggagcag gtgtggaggg ccaaggtggg cgccgcagag
     5641 gaggagctcc agaagtcccg ggtcacagtg aagcatctcg aagagattgt agagaagcta
     5701 aaaggagaac ttgaaagttc ggaccaggtg agggagcaca cgttgcattt ggaggcagag
     5761 ctggaaaagc acatggcggc cgccagcgcc gagtgccaga actacgccaa ggaggtggca
     5821 gggctgaggc aacttctcct agaatctcaa tctcagctcg atgccgccaa gagcgaagcc
     5881 cagaaacaga gcgatgagct tgccctggtc aggcagcagt tgagtgaaat gaagagccac
     5941 gtagaggatg gtgacatagc tggggcccca gcttcctccc cagaggcgcc cccagccgag
     6001 caggaccccg ttcagctgaa gacgcagctg gagtggacag aagccatcct ggaggatgag
     6061 cagacacagc ggcagaagct cacggccgag tttgaggagg ctcagacctc ggcatgtcgg
     6121 ttacaagaag aattggagaa gctccgcaca gccggccccc tagagtcttc agaaacagag
     6181 gaggcctcac agctgaagga gagactagaa aaagagaaga agttaacaag tgacctgggg
     6241 cgcgccgcca cccgcctgca ggaattgctg aaaacaaccc aagaacaatt agcacgtgaa
     6301 aaggatacag tgaaaaagct tcaagagcag ttagagaaag ctgaggacgg ctcctcaagc
     6361 aaggagggca ccagcgtgtg actcgagtct agagggcccg tttaaacccg ctgatcagcc
     6421 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6481 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6541 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6601 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6661 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7081 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7381 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7441 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7501 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7561 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     7621 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     7681 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     7741 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     7801 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     7861 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     7921 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     7981 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8041 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8221 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10381 cacatttccc cgaaaagtgc cacctgacgt c
//
