LOCUS       sequence-181306-        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a biosensor to monitor thioredoxin redox 
            dynamics, mitochondrially locaclized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Fan Y, Makar M, Wang MX, Ai HW
  TITLE     Monitoring thioredoxin redox with a genetically encoded red 
            fluorescent biosensor.
  JOURNAL   Nat Chem Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417.
  PUBMED    28671680
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417."
COMMENT     Sequence Label: pMito-TrxRFP1
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(151..159,160..174)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1323..1342
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgctatcac tgcgacagtc aatacgattc ttcaagccag caacgcgcac cctgtgctca
       61 tcgcgatacc tactcggatc cgggggttct catcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatccg
      181 agctcgagca tggttaaaca gatcgaatct aaaaccgcgt tccaggaagc gctggacgcg
      241 gcgggtgaca aactggttgt tgttgacttc tctgcgacct ggtgcggtcc gtgcaaaatg
      301 atcaaaccgt tcttccactc tctgtctgaa aaatactcta acgttatctt cctggaagtt
      361 gacgttgacg actcccagga cgttgcgtct gaatccgaag ttaaatccat gccgaccttc
      421 cagttcttca aaaaaggtca gaaagttggt gaattctctg gtgcgaacaa agaaaaactg
      481 gaagcgacca tcaacgaact ggttggtgga tccggtggcg gcggtagtgg cggtggaggg
      541 agcggagggg ggggaagcgg gggaggtgga agtggcgggg gaggtagtgg gggcgagctt
      601 agttgcgcta taagtcggct gagctcaccc gtggtttccg agcggatgta ccccgaggac
      661 ggcgccctga agagcgagat caagaagggg ctgaggctga aggacggcgg ccactacgcc
      721 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
      781 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac
      841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaggtaca
      901 ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      961 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1021 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc
     1081 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1141 attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg
     1201 tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc
     1261 ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt cccccccgac
     1321 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcta cgcgtgacca aaggtgctcc
     1381 agtcaggctt aa
//
