LOCUS       sequence-181308-        1380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a biosensor to monitor thioredoxin redox 
            dynamics, nuclear localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Fan Y, Makar M, Wang MX, Ai HW
  TITLE     Monitoring thioredoxin redox with a genetically encoded red 
            fluorescent biosensor.
  JOURNAL   Nat Chem Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417.
  PUBMED    28671680
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417."
COMMENT     Sequence Label: pNuc-TrxRFP1
FEATURES             Location/Qualifiers
     source          1..1380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1242..1261
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1312..1332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1336..1356
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga gctcgagcat ggttaaacag
      121 atcgaatcta aaaccgcgtt ccaggaagcg ctggacgcgg cgggtgacaa actggttgtt
      181 gttgacttct ctgcgacctg gtgcggtccg tgcaaaatga tcaaaccgtt cttccactct
      241 ctgtctgaaa aatactctaa cgttatcttc ctggaagttg acgttgacga ctcccaggac
      301 gttgcgtctg aatccgaagt taaatccatg ccgaccttcc agttcttcaa aaaaggtcag
      361 aaagttggtg aattctctgg tgcgaacaaa gaaaaactgg aagcgaccat caacgaactg
      421 gttggtggat ccggtggcgg cggtagtggc ggtggaggga gcggaggggg gggaagcggg
      481 ggaggtggaa gtggcggggg aggtagtggg ggcgagctta gttgcgctat aagtcggctg
      541 agctcacccg tggtttccga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc
      601 aagaaggggc tgaggctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac
      661 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
      721 gtgtcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      781 tccaccggcg gcatggacga gctgtacaag ggaggtacag gcgggagtct ggtgagcaag
      841 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      901 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      961 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1021 atcctgtccc ctcagttcat gtacggctcc aaggcctaca ttaagcaccc agccgacatc
     1081 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
     1141 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtattcatc
     1201 tacaaggtga agctgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1261 accatgggct gggaggctac gcgtgaccaa aggtgctcca gtcaggctga tccaaaaaag
     1321 aagagaaagg tagacccgaa gaaaaaacga aaggtagatc caaagaagaa gagaaagtaa
//
