LOCUS       sequence-181309-        6622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping
            motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT
            recombination sites flanking kanamycin selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Kenyon A, Gavriouchkina D, Zorman J, Chong-Morrison V, Napolitani G,
            Cerundolo V, Sauka-Spengler T
  TITLE     Generation of a double binary transgenic zebrafish model to study 
            myeloid gene regulation in response to oncogene activation in 
            melanocytes.
  JOURNAL   Dis Model Mech. 2018 Apr 6;11(4). pii: dmm.030056. doi: 
            10.1242/dmm.030056.
  PUBMED    29666124
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2018-04-6"; volume: "11(4). pii: dmm.030056. doi"; 
            pages: " 10.1242/dmm.030056"
COMMENT     Sequence Label: pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
FEATURES             Location/Qualifiers
     source          1..6622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..185
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     425..446
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(495..548)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(564..1526)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(564..1526)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(1542..1568)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1575..1601)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1608..1634)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      1649..1658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1653..1662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1692..1711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1693..1711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1718..1735)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1718..1734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1727..1749)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1875..2330
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1962..1981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2172..2193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2408..2512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2513..2581,2582..3373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2731..2750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4033..4052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4286..4303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4420..4441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4456..4461,4462..4479,4480..4486)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4494..4510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4499..4521
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4518..4534
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4518..4534
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4552..4570
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4552..4569
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4699..4746
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    4771..4819
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4826..5620)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4826..5620)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4937..4956)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5547..5566
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     enhancer        5660..5850
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5807..5827
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5852..5956)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    6050..6097
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    6221..6342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6258..6277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6312..6331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6397..6622,1..485,486..488))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6439..6460)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
       61 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      121 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      181 tcgtctttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      241 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc acatcaggcc gtagccgaag
      301 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      361 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      421 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
      481 ttgctcacag atctgggccc gggattttcc tccacgtccc cgcatgttag aagacttccc
      541 ctgccctctc ctgatccgat atctttttct gcactacgca gggatatttc accgcccatc
      601 cagggtttta ttattccatc ctgctcaagt aataaagccc cctgtttgtc tattccgcgt
      661 gaaatgccaa atatttcttt atcaccaatg ataagtttca ctgggcgatt aataaaatta
      721 tccagctttt cccagcgcga cagataaggt gccaatcctt cttgttcgaa gagttccaac
      781 gcagcacgta attcacgtat tagcatggcc gccaacgtat tacgatcgag attgatcccc
      841 gcttcctgca gcgtgatcca cccctgatta acgacactct cttcaacacg gcgcattgcc
      901 atgttgatcc cggctccaat gactatttgc gccgcatcgc cagttttgcc agtcagctcc
      961 accagaatgc ctgccagctt gcgatcctgc agatagaggt cattaggcca tttaacacga
     1021 actttatctg cacccagctt gcgtaatact tccgccatca cgataccgat aaccagactt
     1081 aaaccaatcg ccgccgccgg gccttgttcc agacgccaga acatcgacaa atataagttt
     1141 gcgccaaaag gcgaaaacca tttccgaccc cggcgaccac ggccagcctg ctggtattct
     1201 gcaatgcaag catcgcccga tttaagctct ccgatacgat caagaaggta ctgattcgtg
     1261 gagtcaatca ctggcagcac ggctacacta ccgccatcca gctgacccaa tatctgttta
     1321 gcattaagta actggatagg ctcaggcagg ctgtatcctt ttcccggaac ggtaaagaca
     1381 tcaacgcccc agtcacgcag tgtctgaatg tgtttattaa tagccgcccg gctcattccc
     1441 agcgtttcac ccaactgctc gccagagtga aattcaccgt tcgctaacag ggcaatcaat
     1501 ttcagtggca cggtgttatc cttcatagga gatctgcctg gaagtgaggc atagtcgggc
     1561 acatcgtaac ccgggaggct agcataatca ggaacatcat atccgggcaa acttgcgtaa
     1621 tctgggacgt cataggtggc catccgcggc cgccatggcg gccgggagca tgcgacgtcg
     1681 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     1741 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     1801 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1861 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1921 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1981 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2041 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2101 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2161 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2221 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2281 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
     2341 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg
     2401 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2461 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2521 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2581 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2641 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2701 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2761 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2821 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2881 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2941 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3001 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3061 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3121 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3181 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3241 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3301 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3361 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3421 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4321 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4381 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4441 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     4501 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg
     4561 acactataga atactcaagc tatgcatcca acgcgttggg agctctccca tatggtcgac
     4621 ctgcaggcgg ccgcgaattc actagtgatt gagctattcc agaagtagtg aggaggcttt
     4681 tttggaggct accatggaga agttactatt ccgaagttcc tattctctag aaagtatagg
     4741 aacttcagag cgcttttgaa gctcggatcc gaacaaacga cccaacaccc gtgcgtttta
     4801 ttctgtcttt ttattgccga tcccctcaga agaactcgtc aagaaggcga tagaaggcga
     4861 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     4921 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     4981 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     5041 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     5101 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     5161 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     5221 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     5281 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     5341 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     5401 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     5461 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     5521 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     5581 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     5641 tctcttgatc gatctttgcg gactatggtt actgactaat tgagatgcat gctttgcata
     5701 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
     5761 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
     5821 acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat tattgaagca
     5881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgaaaac cctggcgtta
     6001 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ggggatcttg aagttcctat
     6061 tccgaagttc ctattctcta gaaagtatag gaacttcaag caggtcggtc gacggatcct
     6121 tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6181 cctgaacccg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     6241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gctcgaagcg
     6361 gccggccgcc ccgactctag agtcgcggcc gctttacttg tacagctcgt ccatgccgag
     6421 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     6481 gtctttgctc agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc
     6541 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     6601 gttgtggcgg atcttgaagt tc
//
