LOCUS       sequence-181340-        8566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ
  TITLE     Enhancer-mediated control of macrophage-specific arginase I 
            expression.
  JOURNAL   J Immunol. 2004 Jun 15;172(12):7565-73.
  PUBMED    15187136
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2004-06-15"; volume: "172(12)"; pages: "7565-73"
COMMENT     Sequence Label: pGL3-mArg1 promoter/enhancer -31/-3810
FEATURES             Location/Qualifiers
     source          1..8566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1794..2249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1881..1900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2091..2112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2380..2428
                     /note="synthetic polyadenylation signal"
     misc_feature    2442..2533
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2482..2501
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      6370..6379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6376..8028
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6442..6460)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    8069..8190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8080..8099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8134..8153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8438..8455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     1801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1981 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2101 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2221 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
     2281 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
     2341 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
     2401 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     2461 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     2521 agaacatttc tctatcgata ggtaccgagc tcttacgcgt agattgccag gaatatacca
     2581 gacactggag ctaggtaaag gcctcctgta cacccataca aggagcgctt ctctgccttc
     2641 aaatatcaac aagttttaaa tccccagtgt gtagtacaga gcaggctggg tggatttgag
     2701 gtctgtgttc ttaggaattg aacctttaaa gcgcagtata cattctctac gctaaggaaa
     2761 gacttgggct gggaaaagtg ggctctgcca agactcaggc cgatgacctc gtcccctcca
     2821 gagcagtggg tctcaagctt cctaactctc caacctttta atacagttct tatgttgcag
     2881 tggccccaat catcaaattg ttttgttgtt acagcataac tataattttc ctagttataa
     2941 agcatactgt aaacaactgt gttttctgat ggttttaggg taaccgctgt gaaaggatct
     3001 atcagcccca aaaggaattg ggatccacag gttaagaacc agtgccttag atgttctgag
     3061 gccacagctt tggagattga aaagacagtc ctttgtgaag actagaatct taggccaagg
     3121 tgcttgctgc caagcctgac accccagtct gactctcaga acccatacag caaggagaac
     3181 ctcctcaggg ccatggtatg tgtttcccaa cacggacaca cacacaatca cacagagtaa
     3241 agggtttttt aattaagcaa gaaatacagt ttacacatat gtctgcataa ggtcacggag
     3301 ggtggtagcc gacgagagac cagctcatct tcaataagga agtcagagag cagaaggctt
     3361 tgtcagcagg gcaagactat actttgttag gaagtgaggc attgttcaga cttccttatg
     3421 ctttcttatg aacaggctgt attagccaac agtcctgtct gtacgtgagt tgtgccactt
     3481 tgggttgagg gtgctgggct aacacagatc ttagttaagc atttccttgt cctttgattc
     3541 ctcaatgacc cagaacaggc aaacaatacg atacttatgt ttgatgaatt agatgactgc
     3601 atctacaact gaacttgtgc actgagttct ggatggtcgc ctgaaggaga acgcagggag
     3661 gcaggcgata ctttaatagg actgactgtg agggtaaatc ggtgtcatac atggaaccgt
     3721 cgtgtgtatg gagataccca gaacaataaa gacccaagaa aaacagatag tcaagaaatc
     3781 atgagtttta cttaatcagg gcgttttgat cagtcagtcc aatgatgcca atgatgaaaa
     3841 gtcaccctca aactgcccga gggaagactt tcaggaaaag ccctggtgat aaagtttaac
     3901 agcaaatacc tatgaatgaa tgtataaagc tagtaacggc cgtagcactg ttttcaaaac
     3961 gtagtcaccg gctcactcta catttgcgag accccgacag aagtaaatgt aaggtcaagc
     4021 gattttgaaa aagaggagca ccagttcctt tcctgcagct ctaacgtcct accaatgggg
     4081 ctgcatggac tggtgcagcc acaggtgatg agcccagagc atagaggaaa ctggaagatg
     4141 gagcagcgta tcgtttataa cagcaacttg gcatctgatg gagatagctc agtggtaacg
     4201 tggggagtct gtccatttgg tgctggtggg gaaactggga gagacaaagg ggaaatggag
     4261 gatttaaaca aaatacaaaa aaaaaaaatg gacgcaacag tcagcaatca ctgtgaatag
     4321 caaattcttc atgcatccaa gacttaagcc cagcatcttt caggcaggaa aacaatctac
     4381 ccctgaatgg catttttctt cttttctttt tacctttggg gcaggttctg tgttgacctt
     4441 tgaactaacc ctataacccc agcaggtgtt gaacctataa tccccctgcc tcatcctctc
     4501 aaatagctgg aatttgtggg ccagcaccaa gaggcccaaa gaaagagaaa gtttaaatta
     4561 aatagaagaa aatgtagagg tattgatagg tggtggtatc acacaccttt aatcccagca
     4621 ctcaggaggc agtgacagat tgctatctgt gagttcaagg ttaggctggt ctatagggct
     4681 agttctaggg tagccagagc cacacaaaga aaacatgtct ccaaaaagca aacaaaaatg
     4741 tagaggtgca catttgggat ctgggagcag gtaaaactta aattcctagc tagcttcaaa
     4801 tatataggaa aattctatct tacataaatc aattttttaa aaaaatctat aaccgtatat
     4861 cagaaaaaca tagtaagatg aaaaatatat ttgaaaagga ggtaccaaaa tggtcctgag
     4921 ggactttggc ttcttgattt attcattcaa taaatgcaat acatttattc agtcaataag
     4981 tcaatgttta ttttgtattg aacgattcta tgtactgatg gtagagggaa cagaatcttt
     5041 cttgaggtat taaaattctg ggtgcttttt gtcctcacaa ggttaaagaa atttacttct
     5101 gacatgtggg ttttcttttt aaaattataa tcatgtaact ttgtaactgg gagaatactt
     5161 ggttgtgttt cttctaaatt aatactttta aaaatatttc tttagtgttg ggggtggctt
     5221 tacacaggga ccggaccaca tcacccacgt ggaggtcaga gatcaacttg gaggagttga
     5281 ctctttcctt gcaccaaatg ggttctgcgg gtcaaactcg ggtcatcggg cttggtgtta
     5341 ccggctggcc tctctcatct gccctagatc tcagggggcc ctgctgacac tctgctggtg
     5401 tgtagattag aatcgcggca gctcggcggg tgcagaatgc ccattgctcc gttttgattc
     5461 ttctgcaacc tgtatgtgac tagcaacctc accaagctgc agtctcttcc tcggcaaaac
     5521 agcataagaa tgattctcag tctgaagtta gtttaaagag gaaacaggaa acgaaagaac
     5581 attaactaga atagcacttg gcacacgaca gacaactcat attagctagt ttgctttccc
     5641 tatcttggga tatcatgatt ccaaaaatga gattttcccg gagactgtat ttcaaaaata
     5701 gaactgcttt gggttgtcag ggaaataaat gatgccttcc tgaaataata ataataataa
     5761 caacaacaac aacaacaaca atataaaaca aaaaccagcc ccatgctttc ctagacagtg
     5821 taacctggtg acacatgaaa tgtgtctcac tttccccaga acttgaagcc ttgactcagg
     5881 atgctcaaca ggagggaagt aagagacacc accccccacc cccgcttgct gttttagcct
     5941 caccttgcaa gtccccagtg gacttaaatc ttggaaaagg tgagcaccct gccctgaggt
     6001 gcccaggccg gaagcctagc acttcacatg aggttatgaa atcacacata attgtcaatt
     6061 gtctgaggag agattaatgt catccagctg gctttttcaa aagggtgtga actggacgga
     6121 tgaataatgc tcagagggaa gaatggtagt tcctctgatg gggaggttct gttgactctg
     6181 tcattcttcc attcggtggg cggagccagt tgttggataa acagatccaa cctgattata
     6241 aggggggaaa aagatgtgcc ctctgtcttt tagggttacg gccggtggag aggagctgga
     6301 cagcccgagc acatgcagcc tcgagatctg cgatctaagt aagcttggca ttccggtact
     6361 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     6421 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     6481 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     6541 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     6601 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     6661 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     6721 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     6781 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     6841 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     6901 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     6961 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     7021 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     7081 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     7141 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     7201 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     7261 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     7321 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     7381 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     7441 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     7501 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     7561 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     7621 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     7681 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     7741 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     7801 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     7861 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     7921 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     7981 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg
     8041 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     8101 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8161 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8221 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     8281 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     8341 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     8401 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggcca
//
