LOCUS       sequence-181350-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD287 (mKate2^SEC^3xMyc
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..56)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tatcctatac gaagttattt taaacttacc tttccggatt gaaagtacag attctcacgg
       61 tgtccgagct tggatgggag gtcgcagtaa cgggcgacgg cgacctcgtg ttgctcgacg
      121 taggtctcct tgtcggcctc cttgatacgc tcaagacgac ggtcgacgta gtagactcct
      181 ggcatcttga ggttcttggc tggcttcttg gaacggtagg tggtctgaaa atttaaatca
      241 gattagttag ttagtaaaat catgtttaaa cttaccttga ggttgcagat gaggtgtcct
      301 cctccgacga gcttgagggc catgtcggca cgtccctcaa gtcctccgtc ggctgggtag
      361 agggtctcgg tggaggcctc ccatccgagg gtcttctttt gcatgactgg tccgttggat
      421 gggaagttga ctccacggat ctgaaaattt aaatatgtat ggttagttag taccgaactg
      481 tttaaactta ccttgacgtt gtagatgagg catccgtctt ggagggaggt gtcttgggtg
      541 gcggtgagga ctcctccgtc ctcgtaggtg gtgacacgct cccaggtgaa tccctctggg
      601 aaggattgct tgaagaagtc tgggattcct tgggtgtggt tgatgaaggt ctgaaaattt
      661 aaataatcag ggttagttag tatatatatg tttaaactta ccttggatcc gtacatgaa
//
