LOCUS       sequence-181366-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   O'Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo
            K, Gautam N
  TITLE     Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration
            Mode.
  JOURNAL   Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 
            10.1016/j.devcel.2018.05.029.
  PUBMED    29937389
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 
            10.1016/j.devcel.2018.05.029."
COMMENT     Sequence Label: Venus-KRasCT
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(7..9,10..12,13..723)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGGQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(52..73)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagcttatgg tgagcaaggg cgaggagctg ttcaccgggg tggtacccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
      481 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgggcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aaggaattcg gaagcgggag tgggagcaaa aagaagaaaa agaagtcaaa gacaaagtgt
      781 gtaattatgt agtctaga
//
