LOCUS       sequence-181369-        1445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization and visualization of the dominant negative form of 
            Rab4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   O'Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo
            K, Gautam N
  TITLE     Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration
            Mode.
  JOURNAL   Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 
            10.1016/j.devcel.2018.05.029.
  PUBMED    29937389
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 
            10.1016/j.devcel.2018.05.029."
COMMENT     Sequence Label: YFP Rab4DN
FEATURES             Location/Qualifiers
     source          1..1445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..729
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             16..729
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(46..74)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     669..689
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnnnccaggn ntgncatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
       61 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      121 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      181 ccatggccaa cacttgtcac tactttcggt tatggtctta tgtgctttgc aagataccca
      241 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      301 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      361 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      421 attcttggac acaaattgga gtacaactat aactcacaca atgtatacat catggcagac
      481 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      541 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      601 ccagacaacc attacctgtc ctatcaatct aagctttcga aagatcccaa cgaaaagaga
      661 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      721 ctatacaaaa gatctatgtc ggaaacatac gattacctgt tcaagttcct gataattggc
      781 agtgctggca gcggtaagaa ttgtctgttg catcacttca tcgagagcaa gttcaaagac
      841 gacagttcgc acaccatcgg agtggagttc ggctcgcgga ttgtgaacgt gggcggcaag
      901 tcggtaaagc tacagatatg ggacacagcc ggtcaggaga gattccgatc ggtgacaaga
      961 tcgtactatc gcggagcagc cggagctctc ctggtctacg atgccacttc gcgggactct
     1021 ttcaatgctt tgacaaactg gctgaatgat gctcgcactc tggccagccc aaacattgtg
     1081 atccttttgg tgggcaataa aaaggacttg gaggaggcgc gtgatgtgac ctttctggaa
     1141 gcaagcacct ttgcccaaga gaacgagctt atcttcctgg agacgagcgc caagacgggg
     1201 gagaatgttg aagaggcctt tctcaagtgc tcaaagacca tactggccaa aattgagacc
     1261 ggagagctgg atcccgagcg catcggcagc ggtattcagt acggcggagc tgctctccgt
     1321 aacttacaga cacgacagcg cagcatcaat aagccggatt gcacctgtcg cgtttaagcg
     1381 gccgctcgag tctagagggc ccgttaaacc cgctgatcag cctcganttn cctncccann
     1441 cgnna
//
