LOCUS       sequence-181378-        5982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the 
            Pumby for A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Adamala KP, Martin-Alarcon DA, Boyden ES
  TITLE     Programmable RNA-binding protein composed of repeats of a single 
            modular unit.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 
            10.1073/pnas.1519368113. Epub 2016 Apr 26.
  PUBMED    27118836
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519368113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: 
            "E2579-88"
COMMENT     Sequence Label: PumA
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     509..528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1342..2552
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2390..2409
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        2598..2616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3762..3883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3773..3792)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3827..3846
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4079..4534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4166..4185)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4376..4397
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4601..4620)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4720..4742
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4780..4798)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4866..4970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4971..5039,5040..5831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5189..5208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
       61 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      661 ctcacatggc tcgacacgtc tccggatacg gggacagccc ccccccaaag cccccaggga
      721 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
      781 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
      841 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
      901 ctggggggat acggggaaaa agctttaggc ttgtgtctga gcctgcatgt ttgatggtgt
      961 ctggatgcaa gcagaagggg tggaagagct tgcctggaga gatacagctg ggtcagtagg
     1021 actgggacag gcagctggag aattgccatg tagatgttca tacaatcgtc aaatcatgaa
     1081 ggctggaaaa gccctccaag atccccaaga ccaaccccaa cccacccacc gtgcccactg
     1141 gccatgtccc tcagtgccac atccccacag ttcttcatca cctccaggga cggtgacccc
     1201 cccacctccg tgggcagctg tgccactgca gcaccgctct ttggagaagg taaatcttgc
     1261 taaatccagc ccgaccctcc cctggcacaa cgtaaggcca ttatctctca tccaactcca
     1321 ggacggagtc agtgaggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     1381 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     1441 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     1501 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     1561 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     1621 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     1681 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     1741 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     1801 ggaagcgtgt ggagagtccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     1861 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     1921 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     1981 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     2041 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     2101 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     2161 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     2221 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     2281 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     2341 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     2401 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     2461 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     2521 aaattctggc cgtttttggc ttttttgtta gatgtgtcca ctcccagttc aattacagct
     2581 cttaaggcta gagtacttaa tacgactcac tataggctag cctcgagaag gaggtgccac
     2641 catgcgaggc atgggccgca gccgcctttt ggaagatttt cgaaacaacc ggtaccccaa
     2701 tttacaactg cgggagattg ccggacatat aatggaattt tcccaagacc agcatgggtc
     2761 cagattcatt cagctgaaac tggagcgtgc cacaccagct gagcgccagc ttgtcttcaa
     2821 tgaaatcctc caggctgcct accaactcat ggtggatgtg tttggttgtt acgtcattca
     2881 gaagttcttt gaatttggca gtcttgaaca gaagctggct ttggcagaac ggattcgagg
     2941 tcacgtcctg tcattggcac tacagatgta tggctgccgt gttatccaga aagctcttga
     3001 gtttattcct tcagaccagc agaatgagat ggttcgggaa ctagatggcc atgtcttgaa
     3061 gtgtgtgaaa gatcagaatg gctgttacgt ggttcagaaa tgcattgaat gtgtacagcc
     3121 ccagtctttg caatttatca tcgatgcgtt taagggacag gtatttgcct tatccacaca
     3181 tccttatggc tgccgagtga ttcagagaat cctggagcac tgtctccctg accagacact
     3241 ccctatttta gaggagcttc accagcacac agagcagctt gtacaggatc aatatggatg
     3301 ttatgtaatc caacatgtac tggagcacgg tcgtcctgag gataaaagca aaattgtagc
     3361 agaaatccga ggcaatgtac ttgtattgag tcagcacaaa tttgcatgca atgttgtgca
     3421 gaagtgtgtt actcacgcct cacgtacgga gcgcgctgtg ctcatcgatg aggtgtgcac
     3481 catgaacgac ggtccccaca gtgccttata caccatgatg aaggaccagt atgcctgcta
     3541 cgtggtccag aagatgattg acgtggcgga gccaggccag cggaagatcg tcatgcataa
     3601 gatccgaccc cacatcgcaa ctcttcgtaa gtacacctat ggcaagcaca ttctggccaa
     3661 gctggagaag tactacatga agaacggtgt tgacttaggc ggacgctaac acgcgtggta
     3721 cctctagagt cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     3781 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3841 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3901 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     3961 ctacaaatgt ggtaaaatcg atcggaggag acggagtgtg gatccgggct ggcgtaatag
     4021 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     4081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4501 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     4561 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4621 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4681 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4741 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4801 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcg cgcggtatca
     5701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt ag
//
