LOCUS       sequence-181386-        5982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the 
            Pumby for G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Adamala KP, Martin-Alarcon DA, Boyden ES
  TITLE     Programmable RNA-binding protein composed of repeats of a single 
            modular unit.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 
            10.1073/pnas.1519368113. Epub 2016 Apr 26.
  PUBMED    27118836
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519368113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: 
            "E2579-88"
COMMENT     Sequence Label: PumG
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1336..2546
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2384..2403
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        2592..2610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3756..3877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3767..3786)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3821..3840
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4073..4528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4160..4179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4370..4391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4595..4614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4714..4736
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4774..4792)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4860..4964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4965..5033,5034..5825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5183..5202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tggctcgaca cgtctccgga tacggggaca gccccccccc aaagccccca gggatgtaat
      721 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
      781 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
      841 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
      901 ggatacgggg aaaaagcttt aggcttgtgt ctgagcctgc atgtttgatg gtgtctggat
      961 gcaagcagaa ggggtggaag agcttgcctg gagagataca gctgggtcag taggactggg
     1021 acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca tgaaggctgg
     1081 aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc actggccatg
     1141 tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga cccccccacc
     1201 tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc
     1261 cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac tccaggacgg
     1321 agtcagtgag gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     1381 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     1441 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     1501 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     1561 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     1621 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     1681 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     1741 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     1801 gtgtggagag tccgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     1861 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     1921 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     1981 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     2041 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     2101 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     2161 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     2221 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     2281 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     2341 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     2401 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     2461 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     2521 tggccgtttt tggctttttt gttagatgtg tccactccca gttcaattac agctcttaag
     2581 gctagagtac ttaatacgac tcactatagg ctagcctcga gaaggaggtg ccaccatgcg
     2641 aggcatgggc cgcagccgcc ttttggaaga ttttcgaaac aaccggtacc ccaatttaca
     2701 actgcgggag attgccggac atataatgga attttcccaa gaccagcatg ggtccagatt
     2761 cattgagctg aaactggagc gtgccacacc agctgagcgc cagcttgtct tcaatgaaat
     2821 cctccaggct gcctaccaac tcatggtgga tgtgtttggt agttacgtca ttgagaagtt
     2881 ctttgaattt ggcagtcttg aacagaagct ggctttggca gaacggattc gaggtcacgt
     2941 cctgtcattg gcactacaga tgtatggctc ccgtgttatc gagaaagctc ttgagtttat
     3001 tccttcagac cagcagaatg agatggttcg ggaactagat ggccatgtct tgaagtgtgt
     3061 gaaagatcag aatggcagtt acgtggttga gaaatgcatt gaatgtgtac agccccagtc
     3121 tttgcaattt atcatcgatg cgtttaaggg acaggtattt gccttatcca cacatcctta
     3181 tggctcccga gtgattgaga gaatcctgga gcactgtctc cctgaccaga cactccctat
     3241 tttagaggag cttcaccagc acacagagca gcttgtacag gatcaatatg gaagttatgt
     3301 aatcgaacat gtactggagc acggtcgtcc tgaggataaa agcaaaattg tagcagaaat
     3361 ccgaggcaat gtacttgtat tgagtcagca caaatttgca agcaatgttg tggagaagtg
     3421 tgttactcac gcctcacgta cggagcgcgc tgtgctcatc gatgaggtgt gcaccatgaa
     3481 cgacggtccc cacagtgcct tatacaccat gatgaaggac cagtatgcca gctacgtggt
     3541 cgagaagatg attgacgtgg cggagccagg ccagcggaag atcgtcatgc ataagatccg
     3601 accccacatc gcaactcttc gtaagtacac ctatggcaag cacattctgg ccaagctgga
     3661 gaagtactac atgaagaacg gtgttgactt aggcggacgc taacacgcgt ggtacctcta
     3721 gagtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     3781 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     3841 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3901 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     3961 atgtggtaaa atcgatcgga ggagacggag tgtggatccg ggctggcgta atagcgaaga
     4021 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     4081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4501 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     4561 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4621 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4681 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4741 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4801 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4861 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4921 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4981 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5041 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5101 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5161 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5221 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5281 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5341 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5401 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5461 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5521 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5581 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5641 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcgcgcggt atcattgcag
     5701 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5761 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5821 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5881 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5941 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa ga
//
