LOCUS       sequence-181387-        5982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the 
            Pumby for C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Adamala KP, Martin-Alarcon DA, Boyden ES
  TITLE     Programmable RNA-binding protein composed of repeats of a single 
            modular unit.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 
            10.1073/pnas.1519368113. Epub 2016 Apr 26.
  PUBMED    27118836
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519368113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: 
            "E2579-88"
COMMENT     Sequence Label: PumC
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1329..2539
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2377..2396
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        2585..2603
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3749..3870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3760..3779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3814..3833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4066..4521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4153..4172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4363..4384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4588..4607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4707..4729
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4767..4785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4853..4957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4958..5026,5027..5818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5176..5195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
       61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg
      661 acacgtctcc ggatacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
      721 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
      781 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
      841 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
      901 gggaaaaagc tttaggcttg tgtctgagcc tgcatgtttg atggtgtctg gatgcaagca
      961 gaaggggtgg aagagcttgc ctggagagat acagctgggt cagtaggact gggacaggca
     1021 gctggagaat tgccatgtag atgttcatac aatcgtcaaa tcatgaaggc tggaaaagcc
     1081 ctccaagatc cccaagacca accccaaccc acccaccgtg cccactggcc atgtccctca
     1141 gtgccacatc cccacagttc ttcatcacct ccagggacgg tgaccccccc acctccgtgg
     1201 gcagctgtgc cactgcagca ccgctctttg gagaaggtaa atcttgctaa atccagcccg
     1261 accctcccct ggcacaacgt aaggccatta tctctcatcc aactccagga cggagtcagt
     1321 gaggatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     1381 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     1441 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     1501 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     1561 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     1621 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     1681 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     1741 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     1801 gagtccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     1861 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     1921 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     1981 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     2041 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     2101 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     2161 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     2221 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     2281 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     2341 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     2401 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     2461 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     2521 ttttggcttt tttgttagat gtgtccactc ccagttcaat tacagctctt aaggctagag
     2581 tacttaatac gactcactat aggctagcct cgagaakgag gtgccaccat gcgaggcatg
     2641 ggccgcagcc gccttttgga agattttcga aacaaccggt accccaattt acaactgcgg
     2701 gagattgccg gacatataat ggaattttcc caagaccagc atgggtccag attcattcgc
     2761 ctgaaactgg agcgtgccac accagctgag cgccagcttg tcttcaatga aatcctccag
     2821 gctgcctacc aactcatggt ggatgtgttt ggtagttacg tcattcgcaa gttctttgaa
     2881 tttggcagtc ttgaacagaa gctggctttg gcagaacgga ttcgaggtca cgtcctgtca
     2941 ttggcactac agatgtatgg ctcccgtgtt atccgcaaag ctcttgagtt tattccttca
     3001 gaccagcaga atgagatggt tcgggaacta gatggccatg tcttgaagtg tgtgaaagat
     3061 cagaatggca gttacgtggt tcgcaaatgc attgaatgtg tacagcccca gtctttgcaa
     3121 tttatcatcg atgcgtttaa gggacaggta tttgccttat ccacacatcc ttatggctcc
     3181 cgagtgattc gcagaatcct ggagcactgt ctccctgacc agacactccc tattttagag
     3241 gagcttcacc agcacacaga gcagcttgta caggatcaat atggaagtta tgtaatccgc
     3301 catgtactgg agcacggtcg tcctgaggat aaaagcaaaa ttgtagcaga aatccgaggc
     3361 aatgtacttg tattgagtca gcacaaattt gcaagctatg ttgtgcgcaa gtgtgttact
     3421 cacgcctcac gtacggagcg cgctgtgctc atcgatgagg tgtgcaccat gaacgacggt
     3481 ccccacagtg ccttatacac catgatgaag gaccagtatg ccagctacgt ggtccgcaag
     3541 atgattgacg tggcggagcc aggccagcgg aagatcgtca tgcataagat ccgaccccac
     3601 atcgcaactc ttcgtaagta cacctatggc aagcacattc tggccaagct ggagaagtac
     3661 tacatgaaga acggtgttga cttaggcgga cgctaacacg cgtggtacct ctagagtcga
     3721 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3781 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3841 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3901 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3961 aaaatcgatc ggaggagacg gagtgtggat ccgggctggc gtaatagcga agaggcccgc
     4021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     4081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4261 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4381 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4501 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4561 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4621 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4681 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4741 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4801 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5161 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5641 tattgctgat aaatctggag ccggtgagcg tgggtcgcgc ggtatcattg cagcactggg
     5701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa gg
//
