LOCUS       sequence-181390-        5982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the 
            Pumby for U).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Adamala KP, Martin-Alarcon DA, Boyden ES
  TITLE     Programmable RNA-binding protein composed of repeats of a single 
            modular unit.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 
            10.1073/pnas.1519368113. Epub 2016 Apr 26.
  PUBMED    27118836
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519368113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: 
            "E2579-88"
COMMENT     Sequence Label: PumU
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     497..516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1330..2540
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2378..2397
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        2586..2604
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3750..3871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3761..3780)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3815..3834
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4067..4522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4154..4173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4364..4385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4589..4608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4708..4730
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4768..4786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4854..4958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4959..5027,5028..5819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5177..5196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
       61 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      121 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc
      661 gacacgtctc cggatacggg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctt gtgtctgagc ctgcatgttt gatggtgtct ggatgcaagc
      961 agaaggggtg gaagagcttg cctggagaga tacagctggg tcagtaggac tgggacaggc
     1021 agctggagaa ttgccatgta gatgttcata caatcgtcaa atcatgaagg ctggaaaagc
     1081 cctccaagat ccccaagacc aaccccaacc cacccaccgt gcccactggc catgtccctc
     1141 agtgccacat ccccacagtt cttcatcacc tccagggacg gtgacccccc cacctccgtg
     1201 ggcagctgtg ccactgcagc accgctcttt ggagaaggta aatcttgcta aatccagccc
     1261 gaccctcccc tggcacaacg taaggccatt atctctcatc caactccagg acggagtcag
     1321 tgaggatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     1381 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     1441 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     1501 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     1561 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     1621 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     1681 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
     1741 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
     1801 agagtccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
     1861 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     1921 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     1981 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     2041 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     2101 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     2161 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     2221 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     2281 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     2341 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     2401 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     2461 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     2521 tttttggctt ttttgttaga tgtgtccact cccagttcaa ttacagctct taaggctaga
     2581 gtacttaata cgactcacta taggctagcc tcgagaagga ggtgccacca tgcgaggcat
     2641 gggccgcagc cgccttttgg aagattttcg aaacaaccgg taccccaatt tacaactgcg
     2701 ggagattgcc ggacatataa tggaattttc ccaagaccag catgggaaca gattcattca
     2761 gctgaaactg gagcgtgcca caccagctga gcgccagctt gtcttcaatg aaatcctcca
     2821 ggctgcctac caactcatgg tggatgtgtt tggtaattac gtcattcaga agttctttga
     2881 atttggcagt cttgaacaga agctggcttt ggcagaacgg attcgaggtc acgtcctgtc
     2941 attggcacta cagatgtatg gcaaccgtgt tatccagaaa gctcttgagt ttattccttc
     3001 agaccagcag aatgagatgg ttcgggaact agatggccat gtcttgaagt gtgtgaaaga
     3061 tcagaatggc aattacgtgg ttcagaaatg cattgaatgt gtacagcccc agtctttgca
     3121 atttatcatc gatgcgttta agggacaggt atttgcctta tccacacatc cttatggcaa
     3181 ccgagtgatt cagagaatcc tggagcactg tctccctgac cagacactcc ctattttaga
     3241 ggagcttcac cagcacacag agcagcttgt acaggatcaa tatggaaatt atgtaatcca
     3301 acatgtactg gagcacggtc gtcctgagga taaaagcaaa attgtagcag aaatccgagg
     3361 caatgtactt gtattgagtc agcacaaatt tgcaaacaat gttgtgcaga agtgtgttac
     3421 tcacgcctca cgtacggagc gcgctgtgct catcgatgag gtgtgcacca tgaacgacgg
     3481 tccccacagt gccttataca ccatgatgaa ggaccagtat gccaactacg tggtccagaa
     3541 gatgattgac gtggcggagc caggccagcg gaagatcgtc atgcataaga tccgacccca
     3601 catcgcaact cttcgtaagt acacctatgg caagcacatt ctggccaagc tggagaagta
     3661 ctacatgaag aacggtgttg acttaggcgg acgctaacac gcgtggtacc tctagagtcg
     3721 acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     3781 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3841 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3901 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3961 taaaatcgat cggaggagac ggagtgtgga tccgggctgg cgtaatagcg aagaggcccg
     4021 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4141 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4261 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4381 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4441 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4501 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4561 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4621 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4741 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4801 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtcgcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa ag
//
