LOCUS       sequence-181396-        5052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCG empty vector containing T7 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5052)
  TITLE     pCGT empty vector partner of pCG and pCGN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCGT
FEATURES             Location/Qualifiers
     source          1..5052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          739..1311
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1443..1462)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1508..1563
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1562..1581)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(1902..2098)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1916..2051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2165..2182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2336..2924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2416..2435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3095..3886,3887..3955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3718..3737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3956..4060)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4128..4146
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4184..4206)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4306..4325
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4392..4847)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4529..4550)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4741..4760
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4977..4999
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4991..5008
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4992..5008
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5015..5034
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5015..5033
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(5052,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctaggatggc atcgatgaca ggtggccaac agatgggttc tagagtcgat cgacctgatg
      721 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt
      781 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
      841 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
      901 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
      961 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
     1021 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag
     1081 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct
     1141 ggctggcgtg gaaatattct tattggtaga aacaactaca ccctggtcat catcctgcct
     1201 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc
     1261 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ctttcctaca gctcctgggc
     1321 aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcactcc tcaggtgcag
     1381 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     1441 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     1501 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     1561 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     1621 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     1681 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     1741 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     1801 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     1861 atagctgtcc ctcttctctt atgaagatcc tcgagaagct gtttgcaaaa gcctaggcct
     1921 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     1981 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     2041 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcagc
     2101 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2161 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2221 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2281 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2341 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2401 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2461 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2521 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2581 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2641 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2701 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2761 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2821 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2881 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2941 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3001 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3061 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4081 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4141 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4261 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4321 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4381 accgcatcag gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4441 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4501 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4561 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4621 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4681 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4741 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4801 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc
     4861 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4921 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     4981 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     5041 cgagctcgcc cc
//
