LOCUS       sequence-181401-       10153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified CAG promoter-containing vector for ubiquitous expression of
            catalytically inactive Cas9 fused to LSD1. For targeted enhancer 
            demethylation in chicken embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, 
            Sauka-Spengler T
  TITLE     Genome and epigenome engineering CRISPR toolkit for in vivo 
            modulation of cis-regulatory interactions and gene expression in the
            chicken embryo.
  JOURNAL   Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 
            10.1242/dev.160333.
  PUBMED    29386245
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2018-02-23"; volume: "145(4). pii: 
            dev.160333. doi"; pages: " 10.1242/dev.160333"
COMMENT     Sequence Label: pX330a dCas9-LSD1
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..25
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(35..61,62..64,65..2548,2549..2554,2555..2620,
                     2621..2623,2624..4138)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4151..4171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6776..6793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6782..6989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6998..7138
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6998..7127
                     /label=AAV2 ITR
     rep_origin      7213..7668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7300..7319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7510..7531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7685..7704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7804..7826
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7864..7882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7950..8054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8055..8123,8124..8915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8273..8292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9086..9674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9575..9594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9736..9984
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9736..9756
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9907..9926
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10003..10078
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 catgcccaag aagaagagga aggtgggaag ggggatggac aagaagtact ccattgggct
       61 cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag
      121 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg
      181 cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg
      241 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga
      301 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga
      361 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca
      421 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc
      481 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct
      541 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt
      601 tcagacttac aatcagcttt tcgaagagaa cccgatcaat gcatccggag ttgacgccaa
      661 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct
      721 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac
      781 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga
      841 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct
      901 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa
      961 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca
     1021 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga
     1081 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca
     1141 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct
     1201 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag
     1261 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt
     1321 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc
     1381 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc
     1441 agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca
     1501 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc
     1561 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata
     1621 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt
     1681 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aggactattt
     1741 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc
     1801 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa
     1861 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag
     1921 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa
     1981 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg
     2041 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc
     2101 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca
     2161 gaaagcacaa gtttctggcc agggggacag tctccacgag cacatcgcta atcttgcagg
     2221 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa
     2281 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac
     2341 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa
     2401 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga
     2461 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga
     2521 catcaatcgg ctctccgact acgacgtggc tgctatcgtg ccccagtctt ttctcaaaga
     2581 tgattctatt gataataaag tgttgacaag atccgataaa gctagaggga agagtgataa
     2641 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc
     2701 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc
     2761 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa
     2821 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact
     2881 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga
     2941 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct
     3001 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt
     3061 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat
     3121 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga
     3181 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac
     3241 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat
     3301 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag
     3361 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa
     3421 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt
     3481 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat
     3541 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga
     3601 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg
     3661 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc
     3721 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc
     3781 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat
     3841 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa
     3901 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat
     3961 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac
     4021 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca
     4081 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag
     4141 cagggctgac cccaagaaga agaggaaggt ggaggccagc ggaggaggat ctttatctgg
     4201 gaagaaggcg gcagccgcgg cggcggcggc tgcagcggca gcaaccggga cggaggctgg
     4261 ccctgggaca gcaggcggct ccgagaacgg gtctgaggtg gccgcgcagc ccgcgggcct
     4321 gtcgggccca gccgaggtcg ggccgggggc ggtgggggag cgcacacccc gcaagaaaga
     4381 gcctccgcgg gcctcgcccc ccgggggcct ggcggaaccg ccggggtccg cagggcctca
     4441 ggccggccct actgtcgtgc ctgggtctgc gacccccatg gaaactggaa tagcagagac
     4501 tccggagggg cgtcggacca gccggcgcaa gcgggcgaag gtagagtaca gagagatgga
     4561 tgaaagcttg gccaacctct cagaagatga gtattattca gaagaagaga gaaatgccaa
     4621 agcagagaag gaaaagaagc ttcccccacc accccctcaa gccccacctg aggaagaaaa
     4681 tgaaagtgag cctgaagaac catcgggtgt ggagggcgca gctttccaga gccgacttcc
     4741 tcatgaccgg atgacttctc aagaagcagc ctgttttcca gatattatca gtggaccaca
     4801 acagacccag aaggtttttc ttttcattag aaaccgcaca ctgcagttgt ggttggataa
     4861 tccaaagatt cagctgacat ttgaggctac tctccaacaa ttagaagcac cttataacag
     4921 tgatactgtg cttgtccacc gagttcacag ttatttagag cgtcatggtc ttatcaactt
     4981 cggcatctat aagaggataa aacccctacc aactaaaaag acaggaaagg taattattat
     5041 aggctctggg gtctcaggct tggcagcagc tcgacagtta caaagttttg gaatggatgt
     5101 cacacttttg gaagccaggg atcgtgtggg tggacgagtt gccacatttc gcaaaggaaa
     5161 ctatgtagct gatcttggag ccatggtggt aacaggtctt ggagggaatc ctatggctgt
     5221 ggtcagcaaa caagtaaata tggaactggc caagatcaag caaaaatgcc cactttatga
     5281 agccaacgga caagctgttc ctaaagagaa agatgaaatg gtagagcaag agtttaaccg
     5341 gttgctagaa gctacatctt accttagtca tcaactagac ttcaatgtcc tcaataataa
     5401 gcatgtgtcc cttggccagg cattggaagt tgtcattcag ttacaagaga agcatgtcaa
     5461 agatgagcag attgaacatt ggaagaagat agtgaaaact caggaagaat tgaaagaact
     5521 tcttaataag atggtaaatt tgaaagagaa aattaaagaa ctccatcagc aatacaaaga
     5581 agcatctgaa gtaaagccac ccagagatat tactgccgag ttcttagtga aaagcaaaca
     5641 cagggatctg accgccctat gcaaggaata tgatgaatta gctgaaacac aaggaaagct
     5701 agaagaaaaa cttcaggagt tggaagcgaa tcccccaagt gatgtatatc tctcatcaag
     5761 agacagacaa atacttgatt ggcattttgc aaatcttgaa tttgctaatg ccacacctct
     5821 ctcaactctc tcccttaagc actgggatca ggatgatgac tttgagttca ctggcagcca
     5881 cctgacagta aggaatggct actcgtgtgt gcctgtggct ttagcagaag gcctagacat
     5941 taaactgaat acagcagtgc gacaggttcg ctacacggct tcaggatgtg aagtgatagc
     6001 tgtgaatacc cgctccacga gtcaaacctt tatttataaa tgcgacgcag ttctctgtac
     6061 ccttcccctg ggtgtgctga agcagcagcc accagccgtt cagtttgtgc cacctctccc
     6121 tgagtggaaa acatctgcag tccaaaggat gggatttggc aaccttaaca aggtggtgtt
     6181 gtgttttgat cgggtgttct gggatccaag tgtcaatttg ttcgggcatg ttggcagtac
     6241 gactgccagc aggggtgagc tcttcctctt ctggaacctc tataaagctc caatactgtt
     6301 ggcactagtg gcaggagaag ctgctggtat catggaaaac ataagtgacg atgtgattgt
     6361 tggccgatgc ctggccattc tcaaagggat ttttggtagc agtgcagtac ctcagcccaa
     6421 agaaactgtg gtgtctcgtt ggcgtgctga tccctgggct cggggctctt attcctatgt
     6481 tgctgcagga tcatctggaa atgactatga tttaatggct cagccaatca ctcctggccc
     6541 ctcgattcca ggtgccccac agccgattcc acgactcttc tttgcgggag aacatacgat
     6601 ccgtaactac ccagccacag tgcatggtgc tctgctgagt gggctgcgag aagcgggaag
     6661 aattgcagac cagtttttgg gggccatgta tacgctgcct cgccaggcca caccaggtgt
     6721 tcctgcacag cagtccccaa gcatgtgata agaattccta gagctcgctg atcagcctcg
     6781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6961 tgggaagaga atagcaggca tgctggggag cggccgcagg aacccctagt gatggagttg
     7021 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     7081 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     7141 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     7201 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7261 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     7321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     7441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7501 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     7561 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     7621 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     7681 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7741 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7801 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8761 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9721 cttttgctca catgtgaggg cctatttccc atgattcctt catatttgca tatacgatac
     9781 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     9841 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     9901 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     9961 atcttgtgga aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata
    10021 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
    10081 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc
    10141 gccaattctg cag
//
