LOCUS       sequence-18147-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pRho-DsRed
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..725)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(110..133)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     525..545
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      722..731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagccaccac cttctgatag gcagcctgca cctgaggagt gcggccgcta caggaacagg
       61 tggtggcggc cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag
      121 gtgatgtcca gcttggcgtc cacgtagtag tagccgggca gctgcacggg cttcttggcc
      181 atgtagatgg acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg
      241 tgggtctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag
      301 cccatggtct tcttctgcat cacggggccg tcggagggga agttcacgcc gatgaacttc
      361 accttgtaga tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt cgccacgccg
      421 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg
      481 tagtcgggga tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg
      541 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttcacggtg
      601 ttgtggccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
      661 acggtgccct ccatgcgcac cttgaagcgc atgaactcgg tgatgacgtt ctcggaggag
      721 gccatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc
      781 ttgagctcga ctctagagga tccccggcgg cgcg
//
