LOCUS       sequence-181491-        9347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of humanized Mb2Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  TITLE     Zhang lab Cpf1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pY116 (pcDNA3.1-huMb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..9347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..34)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        19..348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      199..334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     261..280
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             415..1209
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(469..488)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1079..1098
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1383..1504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1420..1439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1474..1493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1553..1569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1553..1569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1566..1588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1577..1593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1601..1607,1608..1625,1626..1631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1646..1667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1784..1801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1955..2543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2035..2054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2714..3505,3506..3574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3337..3356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3575..3679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3754..3773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3945..4324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4325..4528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4479..4499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4573..4592
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4573..4591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4633..4642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4645..4665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8440..8487
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8494..8574
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(8648..8665)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8654..8878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8924..9347,1..5)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9011..9030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9221..9242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
       61 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
      301 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
      361 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      421 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      481 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      541 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      601 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      661 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      721 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      781 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      841 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      901 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
      961 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1021 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1081 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1141 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1201 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     1261 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1321 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1381 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1441 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1501 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1561 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1621 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1681 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     3721 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3781 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3841 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3901 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3961 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4021 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4081 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4141 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4201 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4261 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4321 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4381 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4441 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4501 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
     4561 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
     4621 aagcttaccg gtgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc
     4681 ccagcagccc tgttccagga cttcacccac ctgtaccccc tgagcaagac cgtcagattc
     4741 gagctgaagc ccatcggccg gaccctggaa cacatccacg ccaagaactt tctgagccag
     4801 gacgagacaa tggccgacat gtaccagaaa gtgaaagtga tcctggacga ctaccaccgg
     4861 gactttatcg ccgacatgat gggcgaagtg aagctgacca agctggccga gttctacgac
     4921 gtgtacctga agttccggaa gaaccccaag gacgacggcc tgcagaagca gctgaaagac
     4981 ctgcaggctg tgctgcggaa agaaagcgtg aagcctatcg gaagcggcgg caagtacaag
     5041 accggctacg acagactgtt cggcgccaag ctgttcaagg acggcaaaga gctgggcgac
     5101 ctggccaagt tcgtgatcgc ccaggaaggc gagagcagcc ctaagctggc ccacctggcc
     5161 catttcgaga agttcagcac ctacttcacc ggcttccacg acaaccgcaa gaacatgtac
     5221 agcgacgagg acaagcacac cgccattgcc taccggctga tccacgagaa cctgccccgg
     5281 ttcatcgaca acctgcagat cctgaccacc atcaagcaga agcacagcgc cctgtacgac
     5341 cagatcatca acgagctgac cgccagcggc ctggatgtgt ctctggcctc tcacctggat
     5401 ggctaccaca agctgctgac acaggaaggc atcaccgcct acaaccggat catcggagaa
     5461 gtgaacggct acaccaacaa gcacaaccag atctgccaca agagcgagcg gattgccaag
     5521 ctgcggcccc tgcacaagca gattctgagc gacggaatgg gcgtgtcctt cctgcccagc
     5581 aagttcgccg acgacagcga gatgtgccag gccgtgaatg agttctaccg gcactacacc
     5641 gacgtgttcg ccaaggtgca gagcctgttc gacggcttcg acgaccacca gaaagacggc
     5701 atctacgtgg aacacaagaa cctgaacgag ctgtccaagc aggccttcgg cgactttgcc
     5761 ctgctgggaa gagtgctgga cggctactat gtggacgtcg tgaaccccga gttcaacgag
     5821 agatttgcca aggccaagac cgacaacgcc aaagccaagc tgacaaaaga gaaggacaag
     5881 ttcatcaagg gcgtgcactc cctggcttct ctggaacagg ccatcgagca ccacacagcc
     5941 agacacgacg acgagtctgt gcaggccgga aagctgggcc agtacttcaa gcacggactg
     6001 gccggcgtgg acaaccccat ccagaagatc cacaacaacc actctacaat caagggcttc
     6061 ctggaaagag agcggcctgc cggcgaaaga gccctgccca agatcaagag cggcaagaac
     6121 cctgagatga cccagctgag acagctgaaa gagctgctgg acaacgccct gaacgtggcc
     6181 cacttcgcca aactgctgac cacaaagacc accctggaca accaggatgg caacttctac
     6241 ggcgagttcg gcgtgctgta cgatgagctg gccaagatcc ctaccctgta caacaaagtg
     6301 cgggactacc tgagccagaa gcccttcagc accgagaagt acaagctgaa tttcggcaac
     6361 cccaccctgc tgaacggctg ggacctgaac aaagagaaag ataacttcgg cgtgatcctg
     6421 cagaaagatg gctgctacta cctggctctg ctggataagg cccacaagaa ggtgttcgat
     6481 aacgccccca acaccggcaa gaatgtgtat cagaaaatgg tgtataagct gctgcctggc
     6541 cccaacaaga tgctgcccaa ggtgttcttc gccaagagca acctggacta ctacaacccc
     6601 agcgccgaac tgctggacaa atacgccaag ggcacacaca agaaaggcga caacttcaac
     6661 ctgaaggact gccacgccct gatcgatttc ttcaaggccg gcattaacaa gcaccccgag
     6721 tggcagcact tcggcttcaa gttcagcccc accagcagct accgggacct gagcgacttc
     6781 tacagagagg tggaacccca gggctaccaa gtgaagttcg tggacatcaa cgccgactac
     6841 atcgacgagc tggtggaaca gggcaagctg tacctgtttc agatctacaa caaggacttt
     6901 agccccaagg cccacggcaa gcccaacctg cacaccctgt actttaaggc cctgttcagc
     6961 gaggacaacc tggccgaccc catctacaaa ctgaacggcg aggcccagat cttctaccgg
     7021 aaggccagcc tggacatgaa cgagacaacc atccacagag ccggcgaggt gctggaaaac
     7081 aagaatcccg acaaccctaa gaaacggcag ttcgtgtacg acatcatcaa ggacaagcgg
     7141 tacacccagg ataagttcat gctgcacgtg cccatcacca tgaacttcgg agtgcagggc
     7201 atgaccatca aagagttcaa caaaaaagtg aaccagagca tccagcagta cgacgaagtg
     7261 aatgtgattg gcatcgaccg gggcgagcgg catctgctgt atctgaccgt gatcaacagc
     7321 aagggcgaga ttctggaaca gagatccctg aacgacatca ccaccgcctc cgccaatggc
     7381 acccaagtga ccacccctta ccacaagatc ctggataagc gcgagatcga gcggctgaac
     7441 gccagagtgg gatggggaga gatcgagaca atcaaagaac tgaagtccgg ctacctgtcc
     7501 cacgtggtgc atcagatcaa ccagctgatg ctgaagtaca acgccatcgt ggtgctggaa
     7561 gatctgaatt ttggcttcaa gaggggccgg ttcaaggtgg aaaagcagat ctaccagaac
     7621 ttcgagaatg ccctgatcaa gaaactgaac cacctggtgc tgaaagacaa ggccgacgac
     7681 gagatcggca gctacaagaa cgccctgcag ctgactaaca acttcaccga tctgaagtcc
     7741 attggcaagc agaccggctt tctgttctac gtgcccgcct ggaataccag caagatcgac
     7801 cccgagacag gcttcgtgga cctgctgaag cctagatacg agaatatcgc ccagtcccag
     7861 gccttcttcg gcaagttcga caagatctgc tacaacaccg acaagggcta cttcgagttc
     7921 cacatcgact acgccaagtt caccgataag gccaaaaaca gccggcagaa gtgggctatc
     7981 tgcagccacg gcgacaagag atacgtgtac gataagaccg ccaaccagaa caagggcgct
     8041 gccaagggaa tcaacgtgaa cgacgaactg aaatccctgt tcgcccgcta ccacatcaac
     8101 gataagcagc ccaatctcgt gatggacatc tgccagaaca acgacaaaga gtttcacaag
     8161 agcctgatgt gcctgctgaa aaccctgctg gccctgcggt acagcaacgc cagctccgac
     8221 gaggatttca tcctgagccc cgtggccaac gacgagggcg tgttcttcaa tagcgccctg
     8281 gccgatgaca cccagcccca gaacgctgat gccaacggcg cctaccacat tgccctgaag
     8341 ggactgtggc tgctgaatga gctgaagaac tccgacgatc tgaacaaagt gaagctggcc
     8401 atcgacaacc agacctggct gaatttcgcc cagaacagaa agaggccggc ggccacgaag
     8461 aaggccggcc aggccaagaa gaagaaggga tcctacccat acgatgttcc agattacgct
     8521 tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta tgcctaagaa
     8581 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
     8641 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8701 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8761 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8821 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8881 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     8941 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9001 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9061 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9121 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9181 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9241 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9301 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgc
//
